##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062935_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2643613
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288350072419828	32.0	32.0	32.0	32.0	32.0
2	30.87453345100058	32.0	32.0	32.0	32.0	32.0
3	30.92822247431829	32.0	32.0	32.0	32.0	32.0
4	31.01009678799431	32.0	32.0	32.0	32.0	32.0
5	30.89205984385763	32.0	32.0	32.0	32.0	32.0
6	34.56722069380049	36.0	36.0	36.0	32.0	36.0
7	34.51877449535919	36.0	36.0	36.0	32.0	36.0
8	34.498016918512654	36.0	36.0	36.0	32.0	36.0
9	34.65482655744241	36.0	36.0	36.0	32.0	36.0
10	34.35479209702782	36.0	36.0	36.0	32.0	36.0
11	34.62438639846301	36.0	36.0	36.0	32.0	36.0
12	34.444065375680935	36.0	36.0	36.0	32.0	36.0
13	34.53222162245382	36.0	36.0	36.0	32.0	36.0
14	34.4347814903316	36.0	36.0	36.0	32.0	36.0
15	34.36731775793204	36.0	36.0	36.0	32.0	36.0
16	34.390920683171096	36.0	36.0	36.0	32.0	36.0
17	34.32633936964299	36.0	36.0	36.0	32.0	36.0
18	34.339533433978424	36.0	36.0	36.0	32.0	36.0
19	34.335620607100964	36.0	36.0	36.0	32.0	36.0
20	34.31600275834625	36.0	36.0	36.0	32.0	36.0
21	34.31213040637945	36.0	36.0	36.0	32.0	36.0
22	34.278981832817436	36.0	36.0	36.0	32.0	36.0
23	34.22377897218693	36.0	36.0	36.0	32.0	36.0
24	34.2167106153586	36.0	36.0	36.0	32.0	36.0
25	34.18970931070471	36.0	36.0	36.0	32.0	36.0
26	34.15075239832759	36.0	36.0	36.0	32.0	36.0
27	34.151577783888946	36.0	36.0	36.0	32.0	36.0
28	34.12678217273103	36.0	36.0	36.0	32.0	36.0
29	34.09569328037046	36.0	36.0	36.0	32.0	36.0
30	34.07457823819144	36.0	36.0	36.0	32.0	36.0
31	34.08988758944672	36.0	36.0	36.0	32.0	36.0
32	34.04740368578911	36.0	36.0	36.0	32.0	36.0
33	34.02493784075052	36.0	36.0	36.0	32.0	36.0
34	34.03216053181763	36.0	36.0	36.0	32.0	36.0
35	34.001695407005485	36.0	36.0	36.0	32.0	36.0
36	33.95636237225343	36.0	36.0	36.0	32.0	36.0
37	33.96546544445045	36.0	36.0	36.0	32.0	36.0
38	33.379908103039284	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	8.0
8	8.0
9	14.0
10	23.0
11	24.0
12	11.0
13	18.0
14	196.0
15	520.0
16	703.0
17	796.0
18	1073.0
19	1305.0
20	1890.0
21	2792.0
22	4436.0
23	6987.0
24	10784.0
25	16429.0
26	24145.0
27	35606.0
28	49514.0
29	67243.0
30	90629.0
31	123333.0
32	168919.0
33	254766.0
34	571172.0
35	1210267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63935553728392	16.693562833827137	11.285028296838245	26.3820533320507
2	17.151298227712807	19.042615962052533	37.25871714217697	26.547368668057693
3	19.338822652616127	22.818619801272266	28.126651571984425	29.715905974127182
4	13.033360102922323	15.132206841792486	35.37406371061305	36.46036934467214
5	15.07552952373315	35.81257693102063	33.62605263575985	15.485840909486376
6	34.88756082125671	34.88264329758546	16.631292018821252	13.598503862336573
7	30.891836960884184	29.894942939789555	20.458380601381972	18.75483949794429
8	28.310906810269042	32.849383945201915	18.97041000213346	19.869299242395584
9	27.550980651013525	14.08465195768953	18.009147371331753	40.355220019965195
10	16.03337428771157	26.286469319017456	31.628897348758812	26.051259044512165
11	37.74309631249785	20.714069825019454	22.177782838311046	19.365051024171652
12	25.239244133043115	23.101424078525188	28.487320382487987	23.17201140594371
13	30.001278726902527	19.152188930764883	24.651622583376398	26.194909758956197
14	23.901565233839314	19.15581406346054	24.65249917245945	32.2901215302407
15	25.45044610443244	26.828392529524315	21.809419706688367	25.911741659354874
16	26.17306490444636	25.291109856801945	23.289280347682396	25.2465448910693
17	24.68967881265257	25.46336303114411	24.63402379356112	25.2129343626422
18	25.420243407523753	24.618266610376974	25.403976897015585	24.557513085083684
19	26.043738251430824	24.483819555596245	24.807757564385657	24.664684628587274
20	26.346297815773532	24.006630721257384	24.390749523445283	25.256321939523797
21	27.031730137407607	23.870216523942773	24.124845987193247	24.973207351456374
22	26.506752434443754	23.882043582227094	24.494016645879178	25.117187337449977
23	25.142990523035667	23.841690698607866	24.968477583618103	26.046841194738356
24	25.358941639046073	24.358017400502487	24.75237935622605	25.53066160422539
25	25.440283718639122	24.03008949062153	24.837676709360156	25.691950081379183
26	25.13379079791716	24.906445755942126	25.043140320205033	24.916623125935683
27	25.768191745199235	24.40478735014941	24.431079416036194	25.395941488615158
28	25.187633159016077	24.29599736103041	24.960506246368393	25.555863233585125
29	25.137893934028817	24.528398962523116	24.918322907114344	25.415384196333722
30	25.053063617707416	24.684729669664556	25.059116142272153	25.20309057035587
31	25.802585705984892	24.255671363548327	24.198435684195235	25.743307246271545
32	25.42319919982931	24.414928146548657	24.227709692990487	25.93416296063154
33	25.061190365402002	24.24534404684893	24.684968922482113	26.00849666526695
34	25.696619492066926	24.2634493763669	24.783405295121515	25.25652583644466
35	25.91788007111123	24.226094069158282	24.924494444766026	24.931531414964457
36	25.14065161868061	24.58470365646918	24.62590320263102	25.648741522219193
37	25.98017073685596	24.305714759136645	24.398361785692938	25.315752718314457
38	25.15311453307539	24.379805753550478	24.696173101323048	25.770906612051085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	84.5
2	130.0
3	130.0
4	329.5
5	529.0
6	529.0
7	674.5
8	820.0
9	821.5
10	823.0
11	823.0
12	1187.0
13	1551.0
14	2307.5
15	3064.0
16	3064.0
17	4907.0
18	6750.0
19	6750.0
20	8756.0
21	10762.0
22	12578.0
23	14394.0
24	14394.0
25	17791.5
26	21189.0
27	21189.0
28	27479.0
29	33769.0
30	42964.0
31	52159.0
32	52159.0
33	66665.0
34	81171.0
35	81171.0
36	92591.0
37	104011.0
38	119679.5
39	135348.0
40	135348.0
41	147712.0
42	160076.0
43	180826.0
44	201576.0
45	201576.0
46	209634.0
47	217692.0
48	217692.0
49	232917.0
50	248142.0
51	254231.0
52	260320.0
53	260320.0
54	254045.5
55	247771.0
56	247771.0
57	237759.0
58	227747.0
59	205847.0
60	183947.0
61	183947.0
62	169263.0
63	154579.0
64	129456.0
65	104333.0
66	104333.0
67	86850.0
68	69367.0
69	69367.0
70	55484.0
71	41601.0
72	32448.5
73	23296.0
74	23296.0
75	17904.0
76	12512.0
77	12512.0
78	10961.0
79	9410.0
80	7220.5
81	5031.0
82	5031.0
83	4813.0
84	4595.0
85	4595.0
86	3173.0
87	1751.0
88	1392.5
89	1034.0
90	1034.0
91	786.5
92	539.0
93	464.5
94	390.0
95	390.0
96	355.5
97	321.0
98	321.0
99	697.5
100	1074.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12150038602473205
2	0.054243945690991835
3	0.011272451754473896
4	0.002496583274480796
5	4.160972124134659E-4
6	2.2696211586189052E-4
7	7.565403862063018E-5
8	6.430593282753565E-4
9	4.5392423172378103E-4
10	7.943674055166168E-4
11	0.0023452751972395354
12	0.0029883345255148916
13	0.013579899932403118
14	0.00900283059585499
15	0.023528406011015985
16	0.01062939242619854
17	0.020615725524121723
18	0.0054849177999956875
19	0.007792365977924908
20	0.006052323089650414
21	0.006203631166891675
22	0.007414095784821758
23	0.009683716943440663
24	0.013466418874472169
25	0.016303445322745802
26	0.018686547539295655
27	0.008700214441372471
28	0.005938842031719469
29	0.01047808434895728
30	0.0033287776993077274
31	0.005938842031719469
32	0.00669538241792577
33	0.008435425306200265
34	0.011802030024818307
35	0.016265618303435488
36	0.014374267337919733
37	0.009343273769647827
38	0.00631711222482262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2643613.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.81218000606657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73259390958493	42.60692929710643
2	12.899672078652117	14.141182959886214
3	4.069010468681927	6.6909400276788915
4	1.68482486788991	3.693956957499163
5	0.9375817109066029	2.5695448754304286
6	0.570530626714919	1.8763216446283266
7	0.3969633156536531	1.5230897299389141
8	0.2694200833742276	1.1814001685726103
9	0.20398271590382158	1.0062663608021947
>10	1.107249431775555	10.708265833990115
>50	0.06738632829893243	2.5631583372402558
>100	0.04987848611701994	5.880195809827673
>500	0.007502200358006233	2.7529498229839238
>1k	0.0034037760883372176	2.8057981744146723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4097	0.15497729811436092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1348105793094526E-4
2	0.0	0.0	0.0	0.0	1.1348105793094526E-4
3	0.0	0.0	0.0	0.0	1.1348105793094526E-4
4	0.0	0.0	0.0	0.0	1.1348105793094526E-4
5	0.0	0.0	0.0	0.0	1.1348105793094526E-4
6	0.0	0.0	0.0	0.0	1.8913509655157543E-4
7	0.0	0.0	0.0	0.0	2.2696211586189052E-4
8	0.0	0.0	0.0	0.0	2.2696211586189052E-4
9	0.0	0.0	0.0	0.0	2.2696211586189052E-4
10	0.0	0.0	0.0	0.0	2.2696211586189052E-4
11	0.0	0.0	0.0	0.0	2.2696211586189052E-4
12	0.0	0.0	0.0	0.0	4.16097212413466E-4
13	0.0	0.0	0.0	0.0	4.5392423172378103E-4
14	0.0	0.0	0.0	3.782701931031509E-5	4.5392423172378103E-4
15	0.0	0.0	0.0	3.782701931031509E-5	5.674052896547263E-4
16	0.0	0.0	0.0	7.565403862063018E-5	6.052323089650414E-4
17	0.0	0.0	0.0	7.565403862063018E-5	6.430593282753565E-4
18	0.0	0.0	0.0	1.5130807724126035E-4	6.808863475856716E-4
19	0.0	0.0	0.0	1.8913509655157543E-4	7.187133668959866E-4
20	0.0	0.0	0.0	2.647891351722056E-4	7.565403862063017E-4
21	0.0	0.0	0.0	2.647891351722056E-4	9.078484634475621E-4
22	0.0	0.0	0.0	2.647891351722056E-4	9.078484634475621E-4
23	0.0	0.0	0.0	4.5392423172378103E-4	9.835025020681922E-4
24	0.0	0.0	0.0	7.943674055166168E-4	9.835025020681922E-4
25	0.0	0.0	0.0	0.0010969835599991375	9.835025020681922E-4
26	0.0	0.0	0.0	0.0013617726951713433	0.0010591565406888224
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3250	0.0	20.751402	1
GGTATCA	1110	0.0	19.483164	1
ATAACGA	360	0.0	17.77721	12
ACGAACG	380	0.0	16.42115	15
CGATAAC	385	0.0	16.206968	10
GTATTAT	240	0.0	16.01949	1
TAACGAA	400	0.0	16.000095	13
GATAACG	405	0.0	15.801965	11
CCGATAA	395	0.0	15.796666	9
TAGGACG	640	0.0	15.497746	4
CGAACGA	415	0.0	15.035949	16
TCCGATA	430	0.0	14.882402	8
AGGACGT	720	0.0	14.664257	5
GTCCTAA	600	0.0	14.4175415	1
TCAACGC	4595	0.0	14.274311	4
TATCAAC	4690	0.0	14.197352	2
TTCCGAT	465	0.0	14.106276	7
AACGCAG	4680	0.0	14.083156	6
AACGAAC	455	0.0	14.066016	14
ATCAACG	4670	0.0	14.04666	3
>>END_MODULE