FastQCFastQC Report
Thu 2 Feb 2017
SRR4062935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643613
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41290.15618776273229099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4150.020.0474728
CTAGCGG4250.019.9522229
GTATCAA31600.019.1895431
GGTATCA13300.017.6839081
TAGCGGC4900.017.63201730
CATACCG600.002441462816.0015415
ACCGTCG5950.013.9848048
GTCCTAA5000.013.7597641
TTAGGAC4150.013.49399953
TTAATAC2850.013.4737073
TCAACGC45650.013.4241554
ATACCGT5750.013.3580616
TACCGTC6000.013.334877
AACGCAG46450.013.3319996
CGGTCCA6250.013.31328310
TATCAAC46750.013.2446932
CAAGACG6100.013.1150254
ATCAACG46900.013.1002373
CAACGCA47100.013.0458425
CGTCGTA6150.013.00938310
Produced by FastQC (version 0.11.5)