##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2643613
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235261363898573	32.0	32.0	32.0	32.0	32.0
2	31.274171749041937	32.0	32.0	32.0	32.0	32.0
3	31.365358696601962	32.0	32.0	32.0	32.0	32.0
4	31.46969772050599	32.0	32.0	32.0	32.0	32.0
5	31.382813596392513	32.0	32.0	32.0	32.0	32.0
6	34.922075961950554	36.0	36.0	36.0	36.0	36.0
7	34.95451981814282	36.0	36.0	36.0	36.0	36.0
8	34.88704246801631	36.0	36.0	36.0	32.0	36.0
9	34.99796377155052	36.0	36.0	36.0	36.0	36.0
10	34.84789717708303	36.0	36.0	36.0	32.0	36.0
11	35.026134687641495	36.0	36.0	36.0	36.0	36.0
12	34.92988724143814	36.0	36.0	36.0	32.0	36.0
13	34.97742672622657	36.0	36.0	36.0	36.0	36.0
14	34.925963066454884	36.0	36.0	36.0	32.0	36.0
15	34.88914035450726	36.0	36.0	36.0	32.0	36.0
16	34.90713504586337	36.0	36.0	36.0	32.0	36.0
17	34.88042349617739	36.0	36.0	36.0	32.0	36.0
18	34.878143661723556	36.0	36.0	36.0	32.0	36.0
19	34.876073388956705	36.0	36.0	36.0	32.0	36.0
20	34.86416582154801	36.0	36.0	36.0	32.0	36.0
21	34.85731270045956	36.0	36.0	36.0	32.0	36.0
22	34.8428748837292	36.0	36.0	36.0	32.0	36.0
23	34.78728051344883	36.0	36.0	36.0	32.0	36.0
24	34.7684861589045	36.0	36.0	36.0	32.0	36.0
25	34.75709001279688	36.0	36.0	36.0	32.0	36.0
26	34.691516496552254	36.0	36.0	36.0	32.0	36.0
27	34.6718131587339	36.0	36.0	36.0	32.0	36.0
28	34.650880064517764	36.0	36.0	36.0	32.0	36.0
29	34.614159863792466	36.0	36.0	36.0	32.0	36.0
30	34.59732986636092	36.0	36.0	36.0	32.0	36.0
31	34.59686081132148	36.0	36.0	36.0	32.0	36.0
32	34.56957996499488	36.0	36.0	36.0	32.0	36.0
33	34.542003689647466	36.0	36.0	36.0	32.0	36.0
34	34.53845173253423	36.0	36.0	36.0	32.0	36.0
35	34.51852218914039	36.0	36.0	36.0	32.0	36.0
36	34.49210115096272	36.0	36.0	36.0	32.0	36.0
37	34.49321704803237	36.0	36.0	36.0	32.0	36.0
38	34.07174121174317	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	7.0
21	51.0
22	154.0
23	577.0
24	1627.0
25	3933.0
26	8547.0
27	16626.0
28	29212.0
29	46372.0
30	71268.0
31	102382.0
32	149804.0
33	242849.0
34	602556.0
35	1367644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.004005082865156	17.88407390355797	11.90599498901222	27.205926024564658
2	16.132202406328005	20.01151454467806	36.49951789463889	27.356765154355045
3	18.273854758620114	23.67521267295932	28.496077148962424	29.554855419458143
4	12.544177297020854	16.052071240489802	35.29039279258014	36.1133586699092
5	14.5892871530645	36.3917005943764	33.24770550584031	15.771306746718789
6	35.01512515655681	35.262325101783205	16.234738193129168	13.487811548530814
7	30.677793892130673	30.042449593471947	20.460575441272262	18.81918107312512
8	28.19723961678745	32.994092026391826	18.865607029346187	19.94306132747454
9	27.50060620183249	13.91699441916029	17.803495278434838	40.77890410057238
10	15.760585655702203	26.539632871980302	31.670513061539925	26.029268410777572
11	37.85170274878275	20.671574121956027	22.070649046836703	19.406074082424517
12	25.03107502566574	23.206859428915546	28.421411392486462	23.34065415293225
13	29.928393371185614	19.180287561327127	24.689647867877635	26.201671199609628
14	23.834444628956376	19.080795169939904	24.602749799422078	32.482010401681634
15	25.411926783534504	26.957576619573288	21.636222851075402	25.994273745816805
16	26.170557555344732	25.364274333752455	23.18127622359106	25.283891887311754
17	24.70944120792264	25.488223881483407	24.533810357264848	25.268524553329097
18	25.42478797010001	24.690073774035763	25.293036461842185	24.592101794022046
19	25.989394060325772	24.55643091481242	24.752790972052264	24.701384052809544
20	26.372657419977884	23.989857819582518	24.32039031431605	25.317094446123544
21	27.020627097037764	23.93344706670046	24.03300789450789	25.012917941753887
22	26.425965686341264	23.98396587698951	24.395637483866768	25.194430952802456
23	25.026970715779346	23.831975088591047	24.942238032264996	26.19881616336461
24	25.33139305942284	24.464851701062145	24.634051958437183	25.569703281077828
25	25.47795420733232	24.087545406642658	24.77917515095829	25.655325235066734
26	25.240929925015443	24.84499421435302	24.914709463684332	24.999366396947206
27	25.748786130491162	24.389736466622182	24.391287375000566	25.47019002788609
28	25.214053645522245	24.300379821100897	24.9106052966149	25.57496123676196
29	25.188406926429852	24.465873030583523	24.834383852704615	25.511336190282012
30	25.08930013583683	24.570805182150337	25.020341479634123	25.319553202378714
31	25.739243981626657	24.265011558045753	24.287972558767112	25.707771901560477
32	25.46896992865446	24.40867857738633	24.202975246376834	25.91937624758238
33	25.058092844905815	24.20153781964304	24.711899964177814	26.028469371273328
34	25.628221679950887	24.237776104142323	24.868201207968035	25.265801007938755
35	25.9707831668251	24.116729642349316	24.844672801957017	25.06781438886857
36	25.134806040067136	24.599024138555833	24.57356655455999	25.692603266817045
37	25.97316626904165	24.318574617389157	24.341346483013965	25.366912630555227
38	25.234007575244615	24.27494060207799	24.687709349068378	25.803342473609014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	44.0
2	72.0
3	72.0
4	245.5
5	419.0
6	419.0
7	543.5
8	668.0
9	687.0
10	706.0
11	706.0
12	1068.5
13	1431.0
14	2177.5
15	2924.0
16	2924.0
17	4827.5
18	6731.0
19	6731.0
20	8668.5
21	10606.0
22	12343.0
23	14080.0
24	14080.0
25	17646.0
26	21212.0
27	21212.0
28	27780.5
29	34349.0
30	43092.0
31	51835.0
32	51835.0
33	66918.0
34	82001.0
35	82001.0
36	93300.0
37	104599.0
38	120180.0
39	135761.0
40	135761.0
41	148037.5
42	160314.0
43	181780.5
44	203247.0
45	203247.0
46	210817.5
47	218388.0
48	218388.0
49	233479.5
50	248571.0
51	254358.0
52	260145.0
53	260145.0
54	254141.0
55	248137.0
56	248137.0
57	238516.0
58	228895.0
59	206348.5
60	183802.0
61	183802.0
62	169251.5
63	154701.0
64	129458.0
65	104215.0
66	104215.0
67	87147.0
68	70079.0
69	70079.0
70	55484.0
71	40889.0
72	31964.0
73	23039.0
74	23039.0
75	17518.0
76	11997.0
77	11997.0
78	10597.0
79	9197.0
80	6900.0
81	4603.0
82	4603.0
83	4213.0
84	3823.0
85	3823.0
86	2520.5
87	1218.0
88	947.0
89	676.0
90	676.0
91	416.5
92	157.0
93	107.5
94	58.0
95	58.0
96	42.0
97	26.0
98	26.0
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008397598286889948
2	0.0
3	0.0
4	1.5130807724126035E-4
5	7.565403862063018E-5
6	1.5130807724126035E-4
7	2.647891351722056E-4
8	0.0025344102937911108
9	0.003934010008272769
10	4.5392423172378103E-4
11	0.005749706935167894
12	4.160972124134659E-4
13	1.1348105793094526E-4
14	7.565403862063018E-5
15	0.0
16	3.782701931031509E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1348105793094526E-4
22	3.782701931031509E-5
23	1.8913509655157543E-4
24	0.0
25	7.565403862063018E-5
26	7.565403862063018E-5
27	3.782701931031509E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.565403862063018E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2643613.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.83248016758163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.93733459022303	40.11957723716969
2	13.840985511301163	14.625071850512066
3	4.452597018724969	7.0572523105806
4	1.8540855036394983	3.9182374240013758
5	1.02418598261823	2.7055142807296373
6	0.640364313544387	2.0299220937216518
7	0.42122686947695676	1.5578122159385672
8	0.29448091489594336	1.2446525676776978
9	0.22506432970225526	1.070163606188201
>10	1.1716022034467783	10.979545591584246
>50	0.0722681575108851	2.6392810431531135
>100	0.053624104034856034	6.011250009959293
>500	0.008360580486486519	2.9898449740392206
>1k	0.0038199203946765663	3.0518747947448617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4129	0.15618776273229099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5130807724126035E-4
2	0.0	0.0	0.0	0.0	1.8913509655157543E-4
3	0.0	0.0	0.0	0.0	1.8913509655157543E-4
4	0.0	0.0	0.0	0.0	1.8913509655157543E-4
5	0.0	0.0	0.0	0.0	2.2696211586189052E-4
6	0.0	0.0	0.0	0.0	2.647891351722056E-4
7	0.0	0.0	0.0	0.0	2.647891351722056E-4
8	0.0	0.0	0.0	0.0	3.026161544825207E-4
9	0.0	0.0	0.0	0.0	3.404431737928358E-4
10	0.0	0.0	0.0	0.0	3.404431737928358E-4
11	0.0	0.0	0.0	0.0	3.404431737928358E-4
12	0.0	0.0	0.0	0.0	5.295782703444112E-4
13	0.0	0.0	0.0	0.0	5.295782703444112E-4
14	0.0	0.0	0.0	0.0	5.295782703444112E-4
15	0.0	0.0	0.0	0.0	6.052323089650414E-4
16	0.0	0.0	0.0	7.565403862063018E-5	7.943674055166168E-4
17	0.0	0.0	0.0	7.565403862063018E-5	7.943674055166168E-4
18	0.0	0.0	0.0	1.1348105793094526E-4	8.700214441372471E-4
19	0.0	0.0	0.0	1.5130807724126035E-4	9.078484634475621E-4
20	0.0	0.0	0.0	1.5130807724126035E-4	0.0010591565406888224
21	0.0	0.0	0.0	1.5130807724126035E-4	0.0010591565406888224
22	0.0	0.0	0.0	2.2696211586189052E-4	0.0010591565406888224
23	0.0	0.0	0.0	4.16097212413466E-4	0.0010969835599991375
24	0.0	0.0	0.0	6.808863475856716E-4	0.0010969835599991375
25	0.0	0.0	0.0	9.456754827578772E-4	0.0010969835599991375
26	0.0	0.0	0.0	0.001248291637240398	0.0011726375986197677
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	415	0.0	20.04747	28
CTAGCGG	425	0.0	19.95222	29
GTATCAA	3160	0.0	19.189543	1
GGTATCA	1330	0.0	17.683908	1
TAGCGGC	490	0.0	17.632017	30
CATACCG	60	0.0024414628	16.001541	5
ACCGTCG	595	0.0	13.984804	8
GTCCTAA	500	0.0	13.759764	1
TTAGGAC	415	0.0	13.4939995	3
TTAATAC	285	0.0	13.473707	3
TCAACGC	4565	0.0	13.424155	4
ATACCGT	575	0.0	13.358061	6
TACCGTC	600	0.0	13.33487	7
AACGCAG	4645	0.0	13.331999	6
CGGTCCA	625	0.0	13.313283	10
TATCAAC	4675	0.0	13.244693	2
CAAGACG	610	0.0	13.115025	4
ATCAACG	4690	0.0	13.100237	3
CAACGCA	4710	0.0	13.045842	5
CGTCGTA	615	0.0	13.009383	10
>>END_MODULE