FastQCFastQC Report
Thu 2 Feb 2017
SRR4062934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556943
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33620.603652438400339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23550.42284398942082047No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21770.3908838067809453No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18130.3255270287982792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14020.25173132618598315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.21258908003152924No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10030.18009024262806067No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8710.156389433029951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8690.15603032985422208No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6530.11724718687549714No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6140.11024467494878291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG200.003751248632.0010813
TCGACGC308.4097317E-426.66756414
GTATAGG401.5830426E-424.0202221
TAAGACC453.5370927E-421.3302214
GCCTTAG555.7887228E-520.3807951
ACCGTCG751.5065252E-619.1989238
TTAGGGC507.192283E-419.1989213
ATTCGTA2050.018.73065624
CCGTCGT802.790035E-618.0006079
GTGCGGA450.00884299117.77837811
ATCGTTT1352.3646862E-1117.7767829
GACTTAG450.00884756617.776787
CGCTTCG1352.3646862E-1117.7767832
TCTATAC450.00884756617.776783
GCGGACC550.001363961617.45356620
CGGACCG550.001363961617.45356621
AAACGCT1404.0017767E-1117.14189529
TTCGTAT2250.017.06570825
GATTAAG2550.016.955451
GCATTCG2200.016.72633422
Produced by FastQC (version 0.11.5)