##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062934_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556943
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097009927407292	32.0	32.0	32.0	32.0	32.0
2	30.773159910439666	32.0	32.0	32.0	32.0	32.0
3	30.86142926654972	32.0	32.0	32.0	32.0	32.0
4	30.879361083629743	32.0	32.0	32.0	32.0	32.0
5	30.849966693180452	32.0	32.0	32.0	32.0	32.0
6	34.48806071716495	36.0	36.0	36.0	32.0	36.0
7	34.359537690571564	36.0	36.0	36.0	32.0	36.0
8	34.336675386888786	36.0	36.0	36.0	32.0	36.0
9	34.4315648100434	36.0	36.0	36.0	32.0	36.0
10	34.21869383401892	36.0	36.0	36.0	32.0	36.0
11	34.48237611389316	36.0	36.0	36.0	32.0	36.0
12	34.322090411406556	36.0	36.0	36.0	32.0	36.0
13	34.39468850492779	36.0	36.0	36.0	32.0	36.0
14	34.288428079713725	36.0	36.0	36.0	32.0	36.0
15	34.27340679387298	36.0	36.0	36.0	32.0	36.0
16	34.26222791201254	36.0	36.0	36.0	32.0	36.0
17	34.209464882402685	36.0	36.0	36.0	32.0	36.0
18	34.216724153100046	36.0	36.0	36.0	32.0	36.0
19	34.19624988553586	36.0	36.0	36.0	32.0	36.0
20	34.15750624390647	36.0	36.0	36.0	32.0	36.0
21	34.12437358939784	36.0	36.0	36.0	32.0	36.0
22	34.09423046882715	36.0	36.0	36.0	32.0	36.0
23	34.065092837148505	36.0	36.0	36.0	32.0	36.0
24	34.04919893059074	36.0	36.0	36.0	32.0	36.0
25	34.025110289562846	36.0	36.0	36.0	32.0	36.0
26	33.97395963321202	36.0	36.0	36.0	32.0	36.0
27	33.97606397782179	36.0	36.0	36.0	32.0	36.0
28	33.9349825745184	36.0	36.0	36.0	32.0	36.0
29	33.91206460984338	36.0	36.0	36.0	32.0	36.0
30	33.863328922349325	36.0	36.0	36.0	32.0	36.0
31	33.889877420130965	36.0	36.0	36.0	32.0	36.0
32	33.82231933968108	36.0	36.0	36.0	32.0	36.0
33	33.75939009916634	36.0	36.0	36.0	27.0	36.0
34	33.75695178860314	36.0	36.0	36.0	27.0	36.0
35	33.68033173951374	36.0	36.0	36.0	27.0	36.0
36	33.62896741677335	36.0	36.0	36.0	27.0	36.0
37	33.5966014475449	36.0	36.0	36.0	27.0	36.0
38	32.980608428510635	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	1.0
8	1.0
9	3.0
10	5.0
11	4.0
12	1.0
13	1.0
14	270.0
15	429.0
16	425.0
17	523.0
18	634.0
19	730.0
20	826.0
21	1124.0
22	1473.0
23	2079.0
24	2838.0
25	4026.0
26	5549.0
27	8057.0
28	11088.0
29	15200.0
30	20294.0
31	26490.0
32	36110.0
33	52936.0
34	115088.0
35	250737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05992622473413	17.531153151987112	11.896980678933781	24.511939944344974
2	15.482565899981857	20.983258449372038	38.50610539326206	25.02807025738405
3	18.71124606961297	26.281831421188446	28.76226253818154	26.244659971017043
4	12.131348879300594	16.275683255854876	36.54218042156182	35.050787443282715
5	13.578433660955719	37.839667326220685	33.57656632108909	15.005332691734507
6	31.941688826325137	36.23279222469804	18.127169207620888	13.698349741355937
7	28.530388208488123	31.921938151660044	21.494120583255377	18.053553056596456
8	28.05647276821064	32.68712484805392	20.283656617128205	18.972745766607236
9	28.085868905559288	14.641560521562388	18.95367919819299	38.318891374685336
10	15.667576399612166	27.36039788846195	32.03792149962294	24.93410421230294
11	35.97609039539907	22.146370017111636	22.52433425325579	19.353205334233508
12	24.337528953369365	24.853033594886252	29.531538972581835	21.277898479162552
13	29.801638466981046	20.448154120769598	25.634537820860466	24.115669591388887
14	23.846403861451744	20.355582530809553	26.28728525792393	29.510728349814773
15	24.90966235632184	27.20186781609195	24.527298850574713	23.361170977011493
16	24.497869367921464	26.710027528520662	25.431648281397866	23.36045482216001
17	22.82124961836174	26.209119807474725	26.856378297803555	24.11325227635998
18	23.76093085058627	25.12955414698964	28.45953565208023	22.649979350343862
19	24.313914213014755	26.19237875993686	27.228308591807014	22.26539843524137
20	24.858324893878343	24.270436050478715	27.71481106394593	23.15642799169701
21	25.9615591803314	24.942719443786046	25.78199630818735	23.313725067695206
22	24.68526835014356	25.440516571108436	26.248556750457443	23.625658328290562
23	22.97593587208895	25.321115414796896	26.85553801758705	24.8474106955271
24	23.248284110377227	26.589554892305706	26.79589263972244	23.36626835759463
25	24.06715010721174	25.084673929049895	26.638795205821395	24.209380757916968
26	23.000391499082312	26.054802688054252	27.408527496524997	23.53627831633844
27	24.233511106322613	26.040510693314655	26.64763238700641	23.078345813356318
28	23.17761641061001	25.522707520811338	27.361790457310725	23.93788561126793
29	22.926774959327403	26.25025588720133	27.57422524538236	23.24874390808891
30	23.50904788068792	26.114780060546643	27.48354359466069	22.892628464104746
31	23.312647690462338	26.07629985563145	26.503479928462152	24.10757252544406
32	22.8554087710383	26.66883341922532	26.477776121788253	23.997981687948126
33	22.77465428602725	26.05913904157187	27.048201533154604	24.11800513924628
34	23.118059446335963	26.36189285868207	27.427487824850232	23.092559870131733
35	24.219900440332438	26.22456236127373	26.89691336297616	22.658623835417668
36	23.15883990302595	26.965969291550685	26.532459369668672	23.34273143575469
37	23.962721542854066	26.9177036757708	26.43735746736339	22.682217314011744
38	23.180171913802482	26.970391824846878	26.329171657027118	23.520264604323522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1025.0
1	721.5
2	418.0
3	418.0
4	843.0
5	1268.0
6	1268.0
7	1430.5
8	1593.0
9	1504.5
10	1416.0
11	1416.0
12	1749.5
13	2083.0
14	2473.5
15	2864.0
16	2864.0
17	3983.0
18	5102.0
19	5102.0
20	5782.0
21	6462.0
22	6317.0
23	6172.0
24	6172.0
25	6675.0
26	7178.0
27	7178.0
28	9446.0
29	11714.0
30	14243.5
31	16773.0
32	16773.0
33	19506.0
34	22239.0
35	22239.0
36	24168.0
37	26097.0
38	29598.5
39	33100.0
40	33100.0
41	35164.5
42	37229.0
43	41240.5
44	45252.0
45	45252.0
46	49770.0
47	54288.0
48	54288.0
49	53194.0
50	52100.0
51	49659.5
52	47219.0
53	47219.0
54	43730.0
55	40241.0
56	40241.0
57	38470.5
58	36700.0
59	32524.5
60	28349.0
61	28349.0
62	26498.5
63	24648.0
64	20380.0
65	16112.0
66	16112.0
67	13548.5
68	10985.0
69	10985.0
70	8964.0
71	6943.0
72	5465.0
73	3987.0
74	3987.0
75	3088.0
76	2189.0
77	2189.0
78	2048.0
79	1907.0
80	1456.0
81	1005.0
82	1005.0
83	1050.0
84	1095.0
85	1095.0
86	732.0
87	369.0
88	309.5
89	250.0
90	250.0
91	180.0
92	110.0
93	103.5
94	97.0
95	97.0
96	91.0
97	85.0
98	85.0
99	182.0
100	279.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11832449640268394
2	0.05458368271079805
3	0.011491301623325905
4	0.002513722230102542
5	1.7955158786446727E-4
6	0.0
7	0.0
8	3.5910317572893454E-4
9	1.7955158786446727E-4
10	5.386547635934018E-4
11	0.002154619054373607
12	0.0023341706422380743
13	0.014543678617021851
14	0.009695785744681234
15	0.02567587706461882
16	0.01185040479905484
17	0.02388036118597415
18	0.00592520239952742
19	0.009336682568952297
20	0.006284305575256354
21	0.006284305575256354
22	0.007182063514578692
23	0.010054888920410168
24	0.01598009131993759
25	0.018134710374311195
26	0.019571123077226936
27	0.009516234156816765
28	0.006284305575256354
29	0.010234440508274635
30	0.0030523769936959435
31	0.006284305575256354
32	0.007541166690307626
33	0.009336682568952297
34	0.012748162738377176
35	0.016339194495666523
36	0.016698297671395457
37	0.009516234156816765
38	0.0064638571631208215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	556943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.73427089186452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.64039362728076	51.634787176887286
2	10.746071447528669	13.268017715301308
3	2.651699435935734	4.911021939055828
4	0.9833318235528077	2.428210926872006
5	0.4723008598247324	1.457857461144028
6	0.26090674665453173	0.9664132665291555
7	0.20154835337816437	0.8709708451679784
8	0.14377422302386036	0.7100637465137871
9	0.10118175430861415	0.5621743646821858
>10	0.6310451876698338	7.516144421325835
>50	0.09639601843261411	4.203231882148599
>100	0.06610844321227428	7.882565083955331
>500	0.003203492842885451	1.198098114211239
>1k	0.0020385863545634686	2.3904430562053776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3362	0.603652438400339	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2355	0.42284398942082047	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2177	0.3908838067809453	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1813	0.3255270287982792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1402	0.25173132618598315	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.21258908003152924	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1003	0.18009024262806067	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	871	0.156389433029951	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	869	0.15603032985422208	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	653	0.11724718687549714	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	614	0.11024467494878291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.7955158786446727E-4	0.0	0.0	0.0
6	0.0	1.7955158786446727E-4	0.0	0.0	1.7955158786446727E-4
7	0.0	1.7955158786446727E-4	0.0	0.0	1.7955158786446727E-4
8	0.0	1.7955158786446727E-4	0.0	0.0	1.7955158786446727E-4
9	0.0	1.7955158786446727E-4	0.0	0.0	3.5910317572893454E-4
10	0.0	1.7955158786446727E-4	0.0	0.0	3.5910317572893454E-4
11	0.0	1.7955158786446727E-4	0.0	0.0	3.5910317572893454E-4
12	0.0	3.5910317572893454E-4	0.0	0.0	3.5910317572893454E-4
13	0.0	3.5910317572893454E-4	0.0	0.0	3.5910317572893454E-4
14	0.0	3.5910317572893454E-4	0.0	0.0	3.5910317572893454E-4
15	0.0	3.5910317572893454E-4	0.0	1.7955158786446727E-4	3.5910317572893454E-4
16	0.0	3.5910317572893454E-4	0.0	1.7955158786446727E-4	3.5910317572893454E-4
17	0.0	3.5910317572893454E-4	0.0	1.7955158786446727E-4	3.5910317572893454E-4
18	0.0	3.5910317572893454E-4	0.0	1.7955158786446727E-4	3.5910317572893454E-4
19	0.0	3.5910317572893454E-4	0.0	5.386547635934018E-4	3.5910317572893454E-4
20	0.0	3.5910317572893454E-4	0.0	5.386547635934018E-4	3.5910317572893454E-4
21	0.0	3.5910317572893454E-4	0.0	0.0010773095271868036	3.5910317572893454E-4
22	0.0	3.5910317572893454E-4	0.0	0.002154619054373607	3.5910317572893454E-4
23	0.0	3.5910317572893454E-4	0.0	0.0035910317572893456	3.5910317572893454E-4
24	0.0	3.5910317572893454E-4	0.0	0.007720718278172093	3.5910317572893454E-4
25	0.0	3.5910317572893454E-4	0.0	0.008798027805358898	3.5910317572893454E-4
26	0.0	3.5910317572893454E-4	0.0	0.01185040479905484	3.5910317572893454E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	20	0.0037512486	32.00108	13
TCGACGC	30	8.4097317E-4	26.667564	14
GTATAGG	40	1.5830426E-4	24.020222	1
TAAGACC	45	3.5370927E-4	21.330221	4
GCCTTAG	55	5.7887228E-5	20.380795	1
ACCGTCG	75	1.5065252E-6	19.198923	8
TTAGGGC	50	7.192283E-4	19.198921	3
ATTCGTA	205	0.0	18.730656	24
CCGTCGT	80	2.790035E-6	18.000607	9
GTGCGGA	45	0.008842991	17.778378	11
ATCGTTT	135	2.3646862E-11	17.77678	29
GACTTAG	45	0.008847566	17.77678	7
CGCTTCG	135	2.3646862E-11	17.77678	32
TCTATAC	45	0.008847566	17.77678	3
GCGGACC	55	0.0013639616	17.453566	20
CGGACCG	55	0.0013639616	17.453566	21
AAACGCT	140	4.0017767E-11	17.141895	29
TTCGTAT	225	0.0	17.065708	25
GATTAAG	255	0.0	16.95545	1
GCATTCG	220	0.0	16.726334	22
>>END_MODULE