FastQCFastQC Report
Thu 2 Feb 2017
SRR4062934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556943
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43650.7837426810283996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31310.5621760216036471No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22420.40255465999213563No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22110.39698856076833716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19080.3425844296454036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.2660954532151405No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9380.16841938941687032No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8540.15333705603625505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.14310261552798043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.13304772660757025No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6930.12442925039007582No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG5960.1070127463672225No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5860.10521723048857781No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5790.10396036937352654No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.10252395667061082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC1300.022.15198130
GTCCTAA805.2332325E-922.0020981
CACCTAG453.535618E-421.3315414
TCTAGCG1350.021.33153728
CTAGCGG1400.020.56969829
ATAGGCC400.00447815520.0019073
TAAGCCC400.00447815520.0019074
GTTAGAC601.13601265E-418.6684473
GCAGGAC601.13601265E-418.6684473
AGCGGCG1550.018.5790831
CTAAGAC701.7831024E-518.287463
GGTTATA701.7831024E-518.287461
GTATTAG802.7883925E-618.0017171
GTAGGAC1600.018.0017173
ATCTATA550.001362576817.4562111
ATTGAGG550.001362576817.4562113
TAGGACG1302.582965E-1017.2324124
CGGTCCA1750.016.45871210
TAGCGTA1203.0893716E-816.00447
AGAACCG600.002435309616.00445
Produced by FastQC (version 0.11.5)