##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556943
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04301876493645	32.0	32.0	32.0	32.0	32.0
2	31.29721174339205	32.0	32.0	32.0	32.0	32.0
3	31.388176168835948	32.0	32.0	32.0	32.0	32.0
4	31.494160084604708	32.0	32.0	32.0	32.0	32.0
5	31.40539696162803	32.0	32.0	32.0	32.0	32.0
6	34.9188390912535	36.0	36.0	36.0	36.0	36.0
7	34.956417443077655	36.0	36.0	36.0	36.0	36.0
8	34.9032683775539	36.0	36.0	36.0	36.0	36.0
9	34.989835584610994	36.0	36.0	36.0	36.0	36.0
10	34.864907180806654	36.0	36.0	36.0	32.0	36.0
11	35.03448647348113	36.0	36.0	36.0	36.0	36.0
12	34.93179373831792	36.0	36.0	36.0	36.0	36.0
13	34.970110047168205	36.0	36.0	36.0	36.0	36.0
14	34.919779941573914	36.0	36.0	36.0	32.0	36.0
15	34.88887552227068	36.0	36.0	36.0	32.0	36.0
16	34.88672090321631	36.0	36.0	36.0	32.0	36.0
17	34.86665960430421	36.0	36.0	36.0	32.0	36.0
18	34.85888142951792	36.0	36.0	36.0	32.0	36.0
19	34.82998978351465	36.0	36.0	36.0	32.0	36.0
20	34.82597141897824	36.0	36.0	36.0	32.0	36.0
21	34.810458161786755	36.0	36.0	36.0	32.0	36.0
22	34.7838270702747	36.0	36.0	36.0	32.0	36.0
23	34.71992286463785	36.0	36.0	36.0	32.0	36.0
24	34.70741350551133	36.0	36.0	36.0	32.0	36.0
25	34.68075368574522	36.0	36.0	36.0	32.0	36.0
26	34.615077305936154	36.0	36.0	36.0	32.0	36.0
27	34.59631236948844	36.0	36.0	36.0	32.0	36.0
28	34.543752233172874	36.0	36.0	36.0	32.0	36.0
29	34.515799641974134	36.0	36.0	36.0	32.0	36.0
30	34.47843136550778	36.0	36.0	36.0	32.0	36.0
31	34.45730532567965	36.0	36.0	36.0	32.0	36.0
32	34.41674821301282	36.0	36.0	36.0	32.0	36.0
33	34.356029252544694	36.0	36.0	36.0	32.0	36.0
34	34.327586485511084	36.0	36.0	36.0	32.0	36.0
35	34.25977164629055	36.0	36.0	36.0	32.0	36.0
36	34.20761191001593	36.0	36.0	36.0	32.0	36.0
37	34.16630427171183	36.0	36.0	36.0	32.0	36.0
38	33.76340128163923	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	13.0
22	68.0
23	134.0
24	378.0
25	998.0
26	2044.0
27	4009.0
28	6987.0
29	11181.0
30	16562.0
31	23724.0
32	33300.0
33	51732.0
34	122935.0
35	282876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45120840957534	18.52254219422542	12.71294638608412	25.313303010115124
2	14.668107867411925	21.86435595743191	37.72630233255468	25.741233842601485
3	17.927687393503465	26.57686693252272	29.327597258606357	26.167848415367462
4	11.68557644139526	17.016463085091292	36.569810555119645	34.728149918393804
5	13.058607433794842	38.16764013552554	33.445253823102185	15.328498607577437
6	32.128422477704184	36.72207030162871	17.512205019185085	13.637302201482019
7	28.22253105372591	31.936050332352973	21.61569151323846	18.22572710068266
8	27.994361948539314	32.77898479162552	20.272745228305173	18.953908031529995
9	27.792621481952327	14.340454720118368	19.001138416344354	38.86578538158495
10	15.519652960198513	27.508937244473813	32.095615498286165	24.875794297041505
11	35.96443975191861	21.98885285699408	22.64335247452928	19.403354916558033
12	24.168715065743285	24.909370685119914	29.54632374461117	21.37559050452563
13	29.85504748258792	20.38689915089749	25.69212896877766	24.065924397736925
14	23.783044225351606	20.169568519579204	26.43376431699474	29.613622938074453
15	24.907575820146764	27.33062449837775	24.495504925997814	23.26629475547767
16	24.315414683369752	26.46195391628946	25.51230556807429	23.7103258322665
17	22.698193531474494	26.21076124486707	27.0302346918805	24.06081053177794
18	23.824161538972568	24.69660270440602	28.844603487250943	22.634632269370474
19	24.306975758740123	25.947718168645622	27.611802284973507	22.133503787640745
20	24.761600379212954	24.013049809405988	28.21078638208937	23.014563429291687
21	25.856419261645307	24.770128254159776	26.11012656636879	23.263325917826126
22	24.384003389933977	25.133810820855995	26.881386425540853	23.60079936366917
23	22.91764672084346	24.882124170919052	27.295122292806074	24.90510681543141
24	23.180828199654183	26.080047688901736	27.185008160619667	23.55411595082441
25	23.915373745607717	24.788892220568354	27.044778370497518	24.25095566332641
26	23.014563429291687	25.61464997315704	27.87771818660078	23.493068410950492
27	24.319544369890636	25.55252512375593	26.99378571954401	23.134144786809422
28	23.133965235221556	25.141531539134164	27.895852896975093	23.82865032866918
29	22.912398575796804	25.60513373900022	28.19480629076943	23.28766139443354
30	23.53795630791661	25.46651991316885	28.061579012573993	22.93394476634054
31	23.247621390339766	25.420734258263412	27.04352150938247	24.288122842014353
32	22.79389452780626	25.906241751848935	27.15897318037932	24.14089053996549
33	22.8664333693035	25.463108432999427	27.44948764954403	24.22097054815304
34	23.189267124283813	25.817363715856022	27.951693440800945	23.04167571905922
35	24.3768213264194	25.519846734764602	27.306564585603915	22.79676735321209
36	23.14294281461478	26.427300459831613	27.178723855044414	23.25103287050919
37	24.087922821545472	26.20411783611608	26.84691252067088	22.861046821667568
38	23.27275861264079	26.059399256297322	26.977805628224072	23.690036502837813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	470.0
1	363.0
2	256.0
3	256.0
4	807.0
5	1358.0
6	1358.0
7	1538.5
8	1719.0
9	1560.5
10	1402.0
11	1402.0
12	1785.5
13	2169.0
14	2621.0
15	3073.0
16	3073.0
17	4416.0
18	5759.0
19	5759.0
20	6463.5
21	7168.0
22	6783.0
23	6398.0
24	6398.0
25	6647.0
26	6896.0
27	6896.0
28	9141.5
29	11387.0
30	13746.5
31	16106.0
32	16106.0
33	19161.0
34	22216.0
35	22216.0
36	24001.0
37	25786.0
38	29190.0
39	32594.0
40	32594.0
41	34844.5
42	37095.0
43	40869.0
44	44643.0
45	44643.0
46	50016.0
47	55389.0
48	55389.0
49	54451.5
50	53514.0
51	50411.0
52	47308.0
53	47308.0
54	44132.5
55	40957.0
56	40957.0
57	38792.5
58	36628.0
59	32318.5
60	28009.0
61	28009.0
62	26423.0
63	24837.0
64	20426.0
65	16015.0
66	16015.0
67	13443.0
68	10871.0
69	10871.0
70	8852.5
71	6834.0
72	5376.0
73	3918.0
74	3918.0
75	2988.5
76	2059.0
77	2059.0
78	1942.0
79	1825.0
80	1404.5
81	984.0
82	984.0
83	926.5
84	869.0
85	869.0
86	561.5
87	254.0
88	191.5
89	129.0
90	129.0
91	78.5
92	28.0
93	19.5
94	11.0
95	11.0
96	8.5
97	6.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009695785744681234
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	8.977579393223365E-4
8	0.0023341706422380743
9	0.005206996048069551
10	7.182063514578691E-4
11	0.005206996048069551
12	7.182063514578691E-4
13	3.5910317572893454E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	3.5910317572893454E-4
22	0.0
23	1.7955158786446727E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	556943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.68704421137605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52118245681602	49.25445465675008
2	11.533855251293351	13.76843456623116
3	2.8350673920061173	5.0765037830669915
4	1.016997532124572	2.428063066511186
5	0.5041435385359044	1.5045418836736046
6	0.299746336728735	1.0734583711515613
7	0.19333165354651724	0.8077576466882588
8	0.14215661826102913	0.6787926687268603
9	0.10355715372861533	0.5562918371703718
>10	0.6708103220670163	7.714891005382076
>50	0.09999869904833938	4.234659511951102
>100	0.07250660687976934	8.32758644332772
>500	0.004833773791984585	1.8086200690004044
>1k	0.0018126651719942196	2.7659444903686143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4365	0.7837426810283996	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3131	0.5621760216036471	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2242	0.40255465999213563	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2211	0.39698856076833716	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1908	0.3425844296454036	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1482	0.2660954532151405	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	938	0.16841938941687032	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	854	0.15333705603625505	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	797	0.14310261552798043	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	741	0.13304772660757025	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	693	0.12442925039007582	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	596	0.1070127463672225	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	586	0.10521723048857781	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	579	0.10396036937352654	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.10252395667061082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.7955158786446727E-4	0.0
16	0.0	0.0	0.0	1.7955158786446727E-4	0.0
17	0.0	0.0	0.0	1.7955158786446727E-4	0.0
18	0.0	0.0	0.0	1.7955158786446727E-4	0.0
19	0.0	0.0	0.0	7.182063514578691E-4	0.0
20	0.0	0.0	0.0	8.977579393223364E-4	0.0
21	0.0	0.0	0.0	0.0014364127029157382	0.0
22	0.0	0.0	0.0	0.002693273817967009	0.0
23	0.0	0.0	0.0	0.004309238108747214	0.0
24	0.0	0.0	0.0	0.008798027805358898	0.0
25	0.0	0.0	0.0	0.0098753373325457	0.0
26	0.0	0.0	0.0	0.012748162738377177	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	130	0.0	22.151981	30
GTCCTAA	80	5.2332325E-9	22.002098	1
CACCTAG	45	3.535618E-4	21.33154	14
TCTAGCG	135	0.0	21.331537	28
CTAGCGG	140	0.0	20.569698	29
ATAGGCC	40	0.004478155	20.001907	3
TAAGCCC	40	0.004478155	20.001907	4
GTTAGAC	60	1.13601265E-4	18.668447	3
GCAGGAC	60	1.13601265E-4	18.668447	3
AGCGGCG	155	0.0	18.57908	31
CTAAGAC	70	1.7831024E-5	18.28746	3
GGTTATA	70	1.7831024E-5	18.28746	1
GTATTAG	80	2.7883925E-6	18.001717	1
GTAGGAC	160	0.0	18.001717	3
ATCTATA	55	0.0013625768	17.456211	1
ATTGAGG	55	0.0013625768	17.456211	3
TAGGACG	130	2.582965E-10	17.232412	4
CGGTCCA	175	0.0	16.458712	10
TAGCGTA	120	3.0893716E-8	16.0044	7
AGAACCG	60	0.0024353096	16.0044	5
>>END_MODULE