FastQCFastQC Report
Thu 2 Feb 2017
SRR4062933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86369
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3850.44576178953096596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT2140.24777408560942002No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG1430.16556866468293022No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT1360.1574639048732763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT1310.15167479072352347No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG1230.142412208083919No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC1220.14125438525396844No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT1210.14009656242401788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC1180.1366230939341662No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG1140.13199180261436397No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1130.13083397978441338No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA1130.13083397978441338No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG1110.12851833412451227No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC1090.12620268846461113No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG1090.12620268846461113No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC1070.12388704280471001No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT1040.12041357431485833No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA1030.11925575148490777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.11925575148490777No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC1030.11925575148490777No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC1000.1157822829950561No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA950.10999316884530329No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT940.10883534601535273No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG920.1065197003554516No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA890.10304623186559991No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG890.10304623186559991No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA880.10188840903564936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTG308.3545665E-426.66618312
TAATTGC308.3545665E-426.66618313
AATAATT308.3545665E-426.66618311
GGGCGCC401.5771682E-423.99956517
AGGGGCG401.5771682E-423.99956532
GGCGCCG401.5771682E-423.99956518
GCATCGA401.5771682E-423.9995658
GCGCCGA502.759504E-522.39959719
AGCATCG453.5007502E-421.3329477
CAAGGGG453.5007502E-421.33294730
AGGGGGC453.5007502E-421.33294714
CGCCGAG453.5007502E-421.33294720
TAAGAGC453.5007502E-421.3329473
GTATCAA2300.020.1852361
GGGGAAT400.00445102819.9996387
GGCAAGG400.00445102819.99963828
CTAAGAG400.00445102819.9996382
GTCACCA400.00445102819.9996389
CCGAGAG400.00445102819.99963822
CGAGAGG400.00445102819.99963823