##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062933_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86369
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.589540228554227	32.0	32.0	32.0	32.0	32.0
2	29.77164260324885	32.0	32.0	32.0	21.0	32.0
3	29.902198705554078	32.0	32.0	32.0	21.0	32.0
4	29.866827218099086	32.0	32.0	32.0	21.0	32.0
5	29.680429320705347	32.0	32.0	32.0	21.0	32.0
6	33.08368743414883	36.0	36.0	36.0	21.0	36.0
7	33.114601303708504	36.0	36.0	36.0	21.0	36.0
8	32.97317324503005	36.0	36.0	36.0	21.0	36.0
9	33.33336034919937	36.0	36.0	36.0	21.0	36.0
10	32.63360696546214	36.0	32.0	36.0	21.0	36.0
11	33.44526392571409	36.0	36.0	36.0	21.0	36.0
12	33.010918269286435	36.0	32.0	36.0	21.0	36.0
13	33.26955273304079	36.0	36.0	36.0	21.0	36.0
14	32.98617559541039	36.0	32.0	36.0	21.0	36.0
15	32.88451875094073	36.0	32.0	36.0	21.0	36.0
16	32.88393983952576	36.0	32.0	36.0	21.0	36.0
17	32.737428938623815	36.0	32.0	36.0	21.0	36.0
18	32.80394586020447	36.0	32.0	36.0	21.0	36.0
19	32.7938728015839	36.0	32.0	36.0	21.0	36.0
20	32.745962092880546	36.0	32.0	36.0	21.0	36.0
21	32.723535064664404	36.0	32.0	36.0	21.0	36.0
22	32.69893132952796	36.0	32.0	36.0	21.0	36.0
23	32.631684979564426	36.0	32.0	36.0	21.0	36.0
24	32.629438803274326	36.0	32.0	36.0	14.0	36.0
25	32.62011832949322	36.0	32.0	36.0	14.0	36.0
26	32.51455962208663	36.0	32.0	36.0	14.0	36.0
27	32.5644849425141	36.0	32.0	36.0	14.0	36.0
28	32.54613345065938	36.0	32.0	36.0	14.0	36.0
29	32.477926107746995	36.0	32.0	36.0	14.0	36.0
30	32.432423670529936	36.0	32.0	36.0	14.0	36.0
31	32.47593465247948	36.0	32.0	36.0	14.0	36.0
32	32.402030821243734	36.0	32.0	36.0	14.0	36.0
33	32.386458104180896	36.0	32.0	36.0	14.0	36.0
34	32.37365258368165	36.0	32.0	36.0	14.0	36.0
35	32.3560073637532	36.0	32.0	36.0	14.0	36.0
36	32.26959904595399	36.0	32.0	36.0	14.0	36.0
37	32.310423878938046	36.0	32.0	36.0	14.0	36.0
38	31.414211117414812	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	1.0
13	1.0
14	86.0
15	139.0
16	120.0
17	165.0
18	245.0
19	303.0
20	391.0
21	463.0
22	570.0
23	715.0
24	809.0
25	1092.0
26	1445.0
27	2112.0
28	2767.0
29	3860.0
30	5203.0
31	7243.0
32	9997.0
33	13850.0
34	19914.0
35	14873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63697042736627	19.35431537962362	11.992444609251878	26.016269583758227
2	16.35952973880813	19.910812532576593	38.428215671512135	25.301442057103145
3	18.421387798580476	24.214109555733092	29.234545601908135	28.129957043778298
4	12.024129307150796	15.420062986291219	37.06812708410522	35.487680622452764
5	14.38363301647582	36.25143280575207	34.107144924683624	15.257789253088491
6	33.47844712802047	36.78634695318922	16.87874121501928	12.856464703771028
7	29.7166807535111	30.948604244578494	21.24025981544304	18.094455186467368
8	27.92552883557758	32.97710984265188	19.65172689275087	19.44563442901967
9	26.859174009193115	14.14164804501615	19.42132014959071	39.57785779620003
10	15.39557016985261	25.927126631082913	32.05432504718128	26.6229781518832
11	37.33486169486031	21.406324174742668	22.610487802054024	18.648326328343
12	24.85613385051815	23.640363573206738	28.957332252648644	22.54617032362647
13	28.78384324987841	19.246358014683743	26.0694351159182	25.900363619519652
14	23.300138953219083	20.237378415933303	25.631079203334878	30.83140342751274
15	24.701222901612006	27.52223457476376	23.024365388178616	24.75217713544562
16	25.81579008314227	26.127284096435766	24.077676648370737	23.97924917205123
17	23.49460373338274	26.69878178702117	25.846495900690165	23.960118578905927
18	24.692859045170852	24.573592246500155	27.36072995912507	23.372818749203923
19	25.176298938153526	25.106821524102873	26.06560983800183	23.651269699741775
20	24.876388100834887	24.535948771986707	25.777278569691635	24.81038455748677
21	26.55511811023622	24.32144511347846	25.546549328392775	23.576887447892542
22	25.1537152186751	24.309584187306772	26.37069973714987	24.166000856868262
23	23.505639315407954	23.990828875147642	27.19377475161537	25.309757057829035
24	23.970818134445025	25.44033350703491	26.35284581089688	24.236002547623183
25	24.705855104687792	24.38970724476561	26.44756346118214	24.45687418936446
26	23.873268794293388	25.555838621520216	26.948909166705263	23.621983417481125
27	24.889702050789168	24.57473047928946	25.806825156038304	24.728742313883068
28	23.629268518625736	24.766382195254693	27.13440405748098	24.46994522863859
29	23.859425660027792	24.935155164427975	26.879342288096343	24.326076887447893
30	23.9095445966444	24.767550918796243	27.37283327350833	23.95007121105103
31	24.645971075576345	25.003763185623473	25.668399661892245	24.681866076907934
32	24.278601204261232	24.90389069013432	25.67739694302918	25.14011116257527
33	23.59248708863105	24.467912642719842	26.829014104078368	25.11058616457074
34	24.246705421868125	24.24323135002432	27.242513375176596	24.26754985293096
35	25.634314963000705	24.486699940940095	26.654545875649948	23.224439220409252
36	23.877624280602614	25.28804845003879	26.30591613881909	24.528411130539503
37	25.02750211332029	24.846856653195456	25.873988211725745	24.251653021758514
38	24.038906901343214	24.636405743399724	26.558591940713296	24.76609541454377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	10.0
2	16.0
3	16.0
4	35.5
5	55.0
6	55.0
7	66.5
8	78.0
9	74.0
10	70.0
11	70.0
12	98.5
13	127.0
14	202.5
15	278.0
16	278.0
17	403.0
18	528.0
19	528.0
20	684.5
21	841.0
22	885.5
23	930.0
24	930.0
25	1081.0
26	1232.0
27	1232.0
28	1521.0
29	1810.0
30	2099.0
31	2388.0
32	2388.0
33	3038.0
34	3688.0
35	3688.0
36	4056.5
37	4425.0
38	4948.5
39	5472.0
40	5472.0
41	5558.0
42	5644.0
43	6222.5
44	6801.0
45	6801.0
46	6744.0
47	6687.0
48	6687.0
49	7090.5
50	7494.0
51	7421.0
52	7348.0
53	7348.0
54	6945.5
55	6543.0
56	6543.0
57	6255.0
58	5967.0
59	5299.5
60	4632.0
61	4632.0
62	4484.0
63	4336.0
64	3655.0
65	2974.0
66	2974.0
67	2485.5
68	1997.0
69	1997.0
70	1658.0
71	1319.0
72	1045.0
73	771.0
74	771.0
75	602.5
76	434.0
77	434.0
78	457.0
79	480.0
80	382.5
81	285.0
82	285.0
83	300.5
84	316.0
85	316.0
86	219.5
87	123.0
88	102.0
89	81.0
90	81.0
91	65.0
92	49.0
93	44.0
94	39.0
95	39.0
96	31.5
97	24.0
98	24.0
99	53.5
100	83.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08452106658639094
2	0.039365976218319075
3	0.002315645659901122
4	0.001157822829950561
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.002315645659901122
12	0.004631291319802244
13	0.017367342449258416
14	0.010420405469555048
15	0.019682988109159538
16	0.01273605112945617
17	0.015051696789357291
18	0.009262582639604488
19	0.011578228299505609
20	0.011578228299505609
21	0.010420405469555048
22	0.009262582639604488
23	0.01273605112945617
24	0.016209519619307854
25	0.019682988109159538
26	0.015051696789357291
27	0.013893873959406732
28	0.011578228299505609
29	0.010420405469555048
30	0.006946936979703366
31	0.006946936979703366
32	0.010420405469555048
33	0.01273605112945617
34	0.017367342449258416
35	0.018525165279208975
36	0.013893873959406732
37	0.013893873959406732
38	0.010420405469555048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	86369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.98531880651623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.09785598250045	66.29693524296913
2	4.5695076640985635	6.578749319779087
3	1.0277773309957698	2.2195463650152254
4	0.4712656619432873	1.3569683567020576
5	0.2975568172679459	1.070986117704269
6	0.20266031878789828	0.8753140594426241
7	0.12706480304955528	0.6402760249626602
8	0.13189004873498142	0.759531776447568
9	0.09811332893699838	0.635644733642858
>10	0.8524600710919532	12.063356065254895
>50	0.09167966802309685	4.39741110815223
>100	0.032168304569507665	3.1052808299274046
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	385	0.44576178953096596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	214	0.24777408560942002	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	143	0.16556866468293022	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	136	0.1574639048732763	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	131	0.15167479072352347	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	123	0.142412208083919	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	122	0.14125438525396844	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	121	0.14009656242401788	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	118	0.1366230939341662	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	114	0.13199180261436397	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	113	0.13083397978441338	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA	113	0.13083397978441338	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	111	0.12851833412451227	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	109	0.12620268846461113	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	109	0.12620268846461113	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	107	0.12388704280471001	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	104	0.12041357431485833	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	103	0.11925575148490777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	103	0.11925575148490777	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	103	0.11925575148490777	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	100	0.1157822829950561	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	95	0.10999316884530329	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	94	0.10883534601535273	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	92	0.1065197003554516	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	89	0.10304623186559991	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	89	0.10304623186559991	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	88	0.10188840903564936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.001157822829950561
13	0.0	0.0	0.0	0.0	0.001157822829950561
14	0.0	0.0	0.0	0.0	0.001157822829950561
15	0.0	0.0	0.0	0.0	0.001157822829950561
16	0.0	0.0	0.0	0.0	0.001157822829950561
17	0.0	0.0	0.0	0.0	0.001157822829950561
18	0.0	0.0	0.0	0.0	0.001157822829950561
19	0.0	0.0	0.0	0.0	0.001157822829950561
20	0.0	0.0	0.0	0.0	0.002315645659901122
21	0.0	0.0	0.0	0.0	0.002315645659901122
22	0.0	0.0	0.0	0.0	0.002315645659901122
23	0.0	0.0	0.0	0.0	0.002315645659901122
24	0.0	0.0	0.0	0.0	0.002315645659901122
25	0.0	0.0	0.0	0.0	0.002315645659901122
26	0.0	0.0	0.0	0.0	0.002315645659901122
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATTG	30	8.3545665E-4	26.666183	12
TAATTGC	30	8.3545665E-4	26.666183	13
AATAATT	30	8.3545665E-4	26.666183	11
GGGCGCC	40	1.5771682E-4	23.999565	17
AGGGGCG	40	1.5771682E-4	23.999565	32
GGCGCCG	40	1.5771682E-4	23.999565	18
GCATCGA	40	1.5771682E-4	23.999565	8
GCGCCGA	50	2.759504E-5	22.399597	19
AGCATCG	45	3.5007502E-4	21.332947	7
CAAGGGG	45	3.5007502E-4	21.332947	30
AGGGGGC	45	3.5007502E-4	21.332947	14
CGCCGAG	45	3.5007502E-4	21.332947	20
TAAGAGC	45	3.5007502E-4	21.332947	3
GTATCAA	230	0.0	20.185236	1
GGGGAAT	40	0.004451028	19.999638	7
GGCAAGG	40	0.004451028	19.999638	28
CTAAGAG	40	0.004451028	19.999638	2
GTCACCA	40	0.004451028	19.999638	9
CCGAGAG	40	0.004451028	19.999638	22
CGAGAGG	40	0.004451028	19.999638	23
>>END_MODULE