FastQCFastQC Report
Thu 2 Feb 2017
SRR4062933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86369
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC2040.23619585730991444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1740.2014611724113976No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC1620.18756729845199088No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT1520.17598907015248527No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT1450.16788431034283133No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA1410.1632530190230291No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG1370.15862172770322686No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC1290.14935914506362238No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT1270.14704349940372125No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG1230.142412208083919No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTG1210.14009656242401788No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA1190.13778091676411675No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG1130.13083397978441338No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1060.12272921997475945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1040.12041357431485833No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGC1010.11694010582500665No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC1000.1157822829950561No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC950.10999316884530329No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG930.10767752318540218No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC920.1065197003554516No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG910.10536187752550105No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA880.10188840903564936No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG870.1007305862056988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA200.003734477632.030
GTCCTAT200.003734477632.014
ATCGTTT200.003734477632.029
ACGGAAT200.003734477632.018
CATCGTT200.003734477632.028
ATGCACC200.003734477632.04
AATCGAG200.003734477632.022
CAGACCA252.8730818E-432.05
TCTAGCG308.3536835E-426.66666628
CTAGCGG308.3536835E-426.66666629
TAGCGGC401.5769746E-424.030
GCCGGTC401.5769746E-424.08
CTTGCGC401.5769746E-424.03
CGGTCCA401.5769746E-424.010
CGCCGGT401.5769746E-424.07
CCAGGCG350.00205119822.85714523
CACGCTG350.00205119822.85714510
CGTTTAT350.00205119822.85714531
CGGACCA350.00205119822.8571459
GCGCAAG400.00445056220.01
Produced by FastQC (version 0.11.5)