FastQCFastQC Report
Thu 2 Feb 2017
SRR4062932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407640
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49260.20459869415693377No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT48090.19973916366234154No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC44730.18578358890864083No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT41960.17427854662657208No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC36100.14993935970493927No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35850.14890099848814606No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG35800.14869332624478743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA35730.14840258510408533No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35640.14802877506603979No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC33670.13984648867770927No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG33640.1397218853316941No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG33060.13731288730873387No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG32560.13523616487514745No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG32480.13490388928577363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29910.12422953597713944No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC29170.12115598677543155No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG28810.11966074662324933No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG28700.11920386768786032No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27980.11621338738349588No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC27130.11268295924639896No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26770.11118771909421675No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC26010.10803110099516539No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA25410.10553903407486169No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT25400.10549749962618996No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC25340.10524829293415959No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG25310.1051236895881444No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT24640.10234088152713862No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC24220.10059643468292602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8300.018.50487128
CTAGCGG8600.017.86009429
TAGGACG1800.017.7740864
AAGACGG10400.017.6882655
GTATCAA44150.017.5591721
CAAGACG10350.017.4649724
TAGCGGC9300.016.51544430
CGTCTTA1900.016.00099815
TACACCG908.60726E-615.99634555
ACGGACC11500.015.7194538
CGCAAGA10800.015.7076192
GTTATTC5400.015.701423
TCGTTTA7400.015.56692130
AGAGCGA11500.015.44444115
GTCCTAT7700.015.3932511
CGGACCA11550.015.3772619
AGCGGCG10100.015.36569831
ATCGTTT7500.015.35968129
ATAACGA4950.015.19255212
GAGCGAA11700.015.17980316
Produced by FastQC (version 0.11.5)