FastQCFastQC Report
Thu 2 Feb 2017
SRR4062932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407640
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53440.2219600937017162No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT51310.21311325613463808No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC48030.19948995697031116No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT41270.17141266966822283No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA38520.15999069628349752No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG38220.15874466282334568No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC37790.15695868153046139No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36610.1520576165871974No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC36600.1520160821385257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35940.14927480852619163No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG35030.14549517369706436No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC33130.13760362844943597No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG32470.1348623548371019No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32460.13482082038843016No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG32110.13336711468491966No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC31250.12979515209915105No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG29160.12111445232675982No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29050.12065757339137081No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28890.11999302221262316No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT28040.11646259407552624No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC27730.11517502616670267No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27020.11222608031100996No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG26670.11077237460749946No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC25640.10649432639431144No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC25560.10616205080493761No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25450.1057051718695486No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA25050.10404379392267946No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC24980.10375305278197736No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC24860.10325463939791664No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG24670.1024654848731538No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC24150.10030569354222392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG8600.018.97623829
TCTAGCG8700.018.94202628
CAAGACG10900.018.6432554
GCGCAAG11050.018.3909421
AAGACGG11550.017.871515
CGCAAGA11200.017.8566682
TATACCG450.0088490717.7791525
TAGCGGC9250.017.64277330
GTATCAA40450.017.1685541
GTATTAG5100.016.3153251
TAAACGC6250.016.1275728
CGGTCCA10650.016.07535710
AGCGAAC600.002443382515.99957424
CGTCGAA600.002443382515.99957422
AAACGCT6500.015.75342729
GACGGAC12900.015.7531557
ACGGACC13150.015.6970318
TCGCGTA6050.015.6045119
CGCGTAA5950.015.59687110
AGACGGA13650.015.3564986
Produced by FastQC (version 0.11.5)