##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062932_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2407640
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253138342941636	32.0	32.0	32.0	32.0	32.0
2	31.32295027495805	32.0	32.0	32.0	32.0	32.0
3	31.3939899652772	32.0	32.0	32.0	32.0	32.0
4	31.486234653021217	32.0	32.0	32.0	32.0	32.0
5	31.41199722549883	32.0	32.0	32.0	32.0	32.0
6	34.946954694223386	36.0	36.0	36.0	36.0	36.0
7	34.965418833380404	36.0	36.0	36.0	36.0	36.0
8	34.90200611387085	36.0	36.0	36.0	36.0	36.0
9	35.002152730474656	36.0	36.0	36.0	36.0	36.0
10	34.85825538701799	36.0	36.0	36.0	32.0	36.0
11	35.03362338223322	36.0	36.0	36.0	36.0	36.0
12	34.92313136515426	36.0	36.0	36.0	32.0	36.0
13	34.984818743666	36.0	36.0	36.0	36.0	36.0
14	34.91920220630991	36.0	36.0	36.0	32.0	36.0
15	34.88914081839478	36.0	36.0	36.0	32.0	36.0
16	34.906095180342575	36.0	36.0	36.0	32.0	36.0
17	34.866002807728734	36.0	36.0	36.0	32.0	36.0
18	34.86998554601186	36.0	36.0	36.0	32.0	36.0
19	34.867526706650494	36.0	36.0	36.0	32.0	36.0
20	34.85380787825422	36.0	36.0	36.0	32.0	36.0
21	34.84081590270971	36.0	36.0	36.0	32.0	36.0
22	34.83019595952884	36.0	36.0	36.0	32.0	36.0
23	34.77111279094881	36.0	36.0	36.0	32.0	36.0
24	34.7436709807114	36.0	36.0	36.0	32.0	36.0
25	34.72577669419016	36.0	36.0	36.0	32.0	36.0
26	34.658679869083414	36.0	36.0	36.0	32.0	36.0
27	34.64649033908724	36.0	36.0	36.0	32.0	36.0
28	34.62351140535961	36.0	36.0	36.0	32.0	36.0
29	34.587378096393145	36.0	36.0	36.0	32.0	36.0
30	34.56628731870213	36.0	36.0	36.0	32.0	36.0
31	34.56990870728182	36.0	36.0	36.0	32.0	36.0
32	34.53743832134372	36.0	36.0	36.0	32.0	36.0
33	34.506221860411024	36.0	36.0	36.0	32.0	36.0
34	34.499703028692	36.0	36.0	36.0	32.0	36.0
35	34.47454229037564	36.0	36.0	36.0	32.0	36.0
36	34.450625508796996	36.0	36.0	36.0	32.0	36.0
37	34.446374457975445	36.0	36.0	36.0	32.0	36.0
38	34.00907195427888	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	15.0
21	56.0
22	200.0
23	572.0
24	1647.0
25	3938.0
26	8327.0
27	15989.0
28	27753.0
29	43623.0
30	65205.0
31	94082.0
32	135406.0
33	218401.0
34	539974.0
35	1252448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36287707314682	18.099594124926114	11.99231865534099	26.545210146586072
2	16.4313601701251	19.727160206675418	35.99259025435696	27.848889368842517
3	18.310004817996045	23.13007758635012	28.20313668156369	30.356780914090148
4	12.224703681998045	15.506350012647276	35.17998797989561	37.08895832545907
5	14.741821867056537	36.2521390241066	32.669792826170024	16.336246282666846
6	35.632350045978676	35.29853740470951	15.68570524306395	13.383407306247866
7	31.425660442560062	29.917910906502165	19.767547145987667	18.8888815049501
8	28.93456676900993	32.31210209072663	18.42934659808339	20.323984542180042
9	27.254440096546322	13.83847223877894	18.09350505431042	40.81358261036432
10	16.195245583386704	25.84808029858607	30.262990662124412	27.69368345590281
11	38.86036253073703	20.663172060875922	21.20792350634678	19.268541902040273
12	25.31409453675495	23.38838735937964	27.45557362307825	23.841944480787163
13	29.452048251419754	18.60241506305555	25.026177097147873	26.919359588376828
14	23.97062018018482	19.362620392841283	24.009787181550056	32.65697224542384
15	25.58787858649964	27.030453057766113	21.415784751873204	25.96588360386104
16	26.865312089847322	25.441137379342425	22.609277134455315	25.084273396354938
17	24.82601219451413	25.782425944078017	23.9472263295177	25.44433553189015
18	26.050780016946057	24.104351148842852	25.267606452791945	24.57726238141915
19	26.005632071239887	24.396919805286505	24.805494176870297	24.79195394660331
20	26.42089348905983	23.45188649465867	24.05554817165357	26.071671844627936
21	27.913018557591666	23.608471366151086	23.77726736555299	24.701242710704257
22	26.772670974623264	23.530156942198122	24.567106849119345	25.130065234059273
23	24.72480255520459	23.35154622689901	25.338682981431987	26.584968236464412
24	25.295517602299345	24.439409546277684	24.649033908723897	25.616038942699078
25	25.315287999867092	23.958855975145784	24.728115498994864	25.997740525992256
26	25.187330824928488	25.25386903933688	24.94809230121293	24.6107078345217
27	26.204354641353888	23.970920877640243	24.31557401901781	25.509150461988057
28	25.03530428137097	24.022486750510875	25.530021099499926	25.41218786861823
29	24.82343705869648	24.265006396305093	25.450441095844894	25.461115449153525
30	24.643717499293917	24.624819325148277	25.603370935854198	25.12809223970361
31	25.73246000232593	24.46607466232493	24.035528567393797	25.765936767955345
32	25.57101560033892	24.05820637636856	23.972811549899486	26.39796647339303
33	24.743524779452077	23.919813593394363	24.832616171852933	26.504045455300627
34	25.989724377398616	24.002799421840475	24.849852968051703	25.15762323270921
35	26.466373710355366	23.710147696499476	25.097564419929892	24.725914173215266
36	24.724751208652457	24.72333903739762	24.554086159060326	25.997823594889603
37	26.360834676280508	24.45286670764732	24.278961971058795	24.907336645013377
38	25.003738100380456	23.828770081905933	25.02259474007742	26.14489707763619
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	38.0
2	60.0
3	60.0
4	236.5
5	413.0
6	413.0
7	611.5
8	810.0
9	988.0
10	1166.0
11	1166.0
12	1599.5
13	2033.0
14	2920.0
15	3807.0
16	3807.0
17	5818.0
18	7829.0
19	7829.0
20	9715.5
21	11602.0
22	12582.5
23	13563.0
24	13563.0
25	15989.0
26	18415.0
27	18415.0
28	24329.5
29	30244.0
30	37539.0
31	44834.0
32	44834.0
33	60027.0
34	75220.0
35	75220.0
36	83784.5
37	92349.0
38	108085.0
39	123821.0
40	123821.0
41	131650.0
42	139479.0
43	163296.0
44	187113.0
45	187113.0
46	187442.5
47	187772.0
48	187772.0
49	202305.5
50	216839.0
51	224497.0
52	232155.0
53	232155.0
54	223832.0
55	215509.0
56	215509.0
57	209512.5
58	203516.0
59	183032.0
60	162548.0
61	162548.0
62	157432.0
63	152316.0
64	128034.5
65	103753.0
66	103753.0
67	88337.5
68	72922.0
69	72922.0
70	58618.5
71	44315.0
72	34409.5
73	24504.0
74	24504.0
75	18342.0
76	12180.0
77	12180.0
78	11666.5
79	11153.0
80	8819.5
81	6486.0
82	6486.0
83	6129.5
84	5773.0
85	5773.0
86	3755.0
87	1737.0
88	1386.0
89	1035.0
90	1035.0
91	619.5
92	204.0
93	142.5
94	81.0
95	81.0
96	56.5
97	32.0
98	32.0
99	34.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010342077719260354
2	0.0
3	0.0
4	2.907411407020983E-4
5	0.0
6	8.306889734345666E-5
7	4.9841338406074E-4
8	0.0023259291256167865
9	0.0037796348291272786
10	0.0010798956654649366
11	0.0049841338406074
12	2.0767224335864164E-4
13	4.153444867172833E-5
14	4.153444867172833E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	8.306889734345666E-5
23	2.0767224335864164E-4
24	0.0
25	0.0
26	4.153444867172833E-5
27	8.306889734345666E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2407640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.84343628088516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48319553445441	32.503955603842456
2	15.660732807528147	14.045621475327122
3	5.442210658083469	7.321422806187602
4	2.3746460592633514	4.259491569929245
5	1.213456837000887	2.720778717482686
6	0.7109369843651304	1.9128514414861426
7	0.4484429060912098	1.4076804619441294
8	0.2842788820141974	1.0198433545283943
9	0.211742503340019	0.854573531083493
>10	0.9856234490900491	7.684378540872283
>50	0.07420983654201231	2.3223132962495363
>100	0.08098737131823451	8.030109862818128
>500	0.01695767541163801	5.34806895762213
>1k	0.012392147416196979	10.131239305054624
>5k	1.8634808144657114E-4	0.4376710755722099
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5344	0.2219600937017162	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5131	0.21311325613463808	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4803	0.19948995697031116	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4127	0.17141266966822283	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3852	0.15999069628349752	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3822	0.15874466282334568	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3779	0.15695868153046139	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3661	0.1520576165871974	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3660	0.1520160821385257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3594	0.14927480852619163	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3503	0.14549517369706436	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3313	0.13760362844943597	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3247	0.1348623548371019	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3246	0.13482082038843016	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	3211	0.13336711468491966	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	3125	0.12979515209915105	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2916	0.12111445232675982	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2905	0.12065757339137081	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2889	0.11999302221262316	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2804	0.11646259407552624	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2773	0.11517502616670267	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2702	0.11222608031100996	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2667	0.11077237460749946	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	2564	0.10649432639431144	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	2556	0.10616205080493761	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2545	0.1057051718695486	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2505	0.10404379392267946	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	2498	0.10375305278197736	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	2486	0.10325463939791664	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2467	0.1024654848731538	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2415	0.10030569354222392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.306889734345666E-5
2	4.153444867172833E-5	0.0	0.0	4.153444867172833E-5	8.306889734345666E-5
3	4.153444867172833E-5	0.0	0.0	4.153444867172833E-5	8.306889734345666E-5
4	4.153444867172833E-5	0.0	0.0	4.153444867172833E-5	8.306889734345666E-5
5	4.153444867172833E-5	0.0	0.0	4.153444867172833E-5	8.306889734345666E-5
6	4.153444867172833E-5	0.0	0.0	4.153444867172833E-5	1.24603346015185E-4
7	1.24603346015185E-4	0.0	0.0	1.24603346015185E-4	1.24603346015185E-4
8	1.24603346015185E-4	0.0	0.0	1.24603346015185E-4	1.24603346015185E-4
9	2.4920669203037E-4	0.0	0.0	2.4920669203037E-4	1.24603346015185E-4
10	2.4920669203037E-4	0.0	0.0	2.4920669203037E-4	1.24603346015185E-4
11	2.4920669203037E-4	0.0	0.0	2.907411407020983E-4	1.24603346015185E-4
12	2.4920669203037E-4	0.0	0.0	4.1534448671728334E-4	3.7381003804555496E-4
13	2.4920669203037E-4	0.0	0.0	4.5687893538901166E-4	5.399478327324683E-4
14	2.4920669203037E-4	0.0	0.0	5.399478327324683E-4	5.814822814041966E-4
15	2.4920669203037E-4	0.0	0.0	5.399478327324683E-4	8.72223422106295E-4
16	2.4920669203037E-4	0.0	0.0	6.23016730075925E-4	9.552923194497516E-4
17	2.4920669203037E-4	0.0	0.0	7.476200760911099E-4	9.552923194497516E-4
18	2.4920669203037E-4	0.0	0.0	8.306889734345667E-4	9.9682676812148E-4
19	2.907411407020983E-4	0.0	0.0	9.137578707780233E-4	9.9682676812148E-4
20	4.1534448671728334E-4	0.0	0.0	0.0012044990114801217	0.0012044990114801217
21	4.1534448671728334E-4	0.0	0.0	0.00124603346015185	0.0012875679088235783
22	4.1534448671728334E-4	0.0	0.0	0.0016613779468691334	0.0013291023574953066
23	4.1534448671728334E-4	0.0	0.0	0.0019521190875712315	0.0014537057035104916
24	4.1534448671728334E-4	0.0	0.0	0.00274127361233407	0.0014537057035104916
25	4.1534448671728334E-4	0.0	0.0	0.0031981525477230814	0.0014537057035104916
26	4.1534448671728334E-4	0.0	0.0	0.003530428137096908	0.0015783090495256765
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	860	0.0	18.976238	29
TCTAGCG	870	0.0	18.942026	28
CAAGACG	1090	0.0	18.643255	4
GCGCAAG	1105	0.0	18.390942	1
AAGACGG	1155	0.0	17.87151	5
CGCAAGA	1120	0.0	17.856668	2
TATACCG	45	0.00884907	17.779152	5
TAGCGGC	925	0.0	17.642773	30
GTATCAA	4045	0.0	17.168554	1
GTATTAG	510	0.0	16.315325	1
TAAACGC	625	0.0	16.12757	28
CGGTCCA	1065	0.0	16.075357	10
AGCGAAC	60	0.0024433825	15.999574	24
CGTCGAA	60	0.0024433825	15.999574	22
AAACGCT	650	0.0	15.753427	29
GACGGAC	1290	0.0	15.753155	7
ACGGACC	1315	0.0	15.697031	8
TCGCGTA	605	0.0	15.604511	9
CGCGTAA	595	0.0	15.596871	10
AGACGGA	1365	0.0	15.356498	6
>>END_MODULE