FastQCFastQC Report
Thu 2 Feb 2017
SRR4062931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166223
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36770.16974245033867705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21700.021.9977281
GGTATCA7650.021.5673661
GTCCTAA4100.015.6277861
TAGGACG5000.015.0361714
TATACCG650.00417085214.765135
TATCAAC32400.014.473472
TCAACGC32500.014.1748514
ATCAACG32800.014.0959243
ACTATAC2300.013.91142753
GTAGGAC5950.013.7126923
GTTGTCG1301.3378922E-613.5384519
CAACGCA34050.013.5292815
TACTATA1701.1439624E-813.1804172
CTTATAC1954.2200554E-1013.1266813
ACCGTCG1105.710393E-513.089698
AACGCAG35450.013.0855256
GTATTAG1852.6448106E-912.9879571
AGGACGT5550.012.969375
GTATAGG2750.012.8147841
CTAATCG1003.7463297E-412.80028714
Produced by FastQC (version 0.11.5)