##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062931_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166223
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301893203054348	32.0	32.0	32.0	32.0	32.0
2	30.952882044000088	32.0	32.0	32.0	32.0	32.0
3	30.986490310554363	32.0	32.0	32.0	32.0	32.0
4	31.056918424372746	32.0	32.0	32.0	32.0	32.0
5	30.976596130684605	32.0	32.0	32.0	32.0	32.0
6	34.632996695169425	36.0	36.0	36.0	32.0	36.0
7	34.5900897553022	36.0	36.0	36.0	32.0	36.0
8	34.58201671757709	36.0	36.0	36.0	32.0	36.0
9	34.71733981219847	36.0	36.0	36.0	32.0	36.0
10	34.4681844851615	36.0	36.0	36.0	32.0	36.0
11	34.6887721162595	36.0	36.0	36.0	32.0	36.0
12	34.54378981296016	36.0	36.0	36.0	32.0	36.0
13	34.59248840031705	36.0	36.0	36.0	32.0	36.0
14	34.518996889978546	36.0	36.0	36.0	32.0	36.0
15	34.467961516427444	36.0	36.0	36.0	32.0	36.0
16	34.48358871639716	36.0	36.0	36.0	32.0	36.0
17	34.438080936265564	36.0	36.0	36.0	32.0	36.0
18	34.44464166431619	36.0	36.0	36.0	32.0	36.0
19	34.43598004452912	36.0	36.0	36.0	32.0	36.0
20	34.420366231916105	36.0	36.0	36.0	32.0	36.0
21	34.41761951562697	36.0	36.0	36.0	32.0	36.0
22	34.39050550197279	36.0	36.0	36.0	32.0	36.0
23	34.335188944074545	36.0	36.0	36.0	32.0	36.0
24	34.32604768761111	36.0	36.0	36.0	32.0	36.0
25	34.30427384438259	36.0	36.0	36.0	32.0	36.0
26	34.26905771012495	36.0	36.0	36.0	32.0	36.0
27	34.270315198389085	36.0	36.0	36.0	32.0	36.0
28	34.24947246890094	36.0	36.0	36.0	32.0	36.0
29	34.21911917655754	36.0	36.0	36.0	32.0	36.0
30	34.203449967985755	36.0	36.0	36.0	32.0	36.0
31	34.21153962449849	36.0	36.0	36.0	32.0	36.0
32	34.18208559321916	36.0	36.0	36.0	32.0	36.0
33	34.15461150583296	36.0	36.0	36.0	32.0	36.0
34	34.15523978833204	36.0	36.0	36.0	32.0	36.0
35	34.13181699206407	36.0	36.0	36.0	32.0	36.0
36	34.10232372198061	36.0	36.0	36.0	32.0	36.0
37	34.09177217673342	36.0	36.0	36.0	32.0	36.0
38	33.555376801003405	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	3.0
8	5.0
9	9.0
10	16.0
11	22.0
12	9.0
13	11.0
14	154.0
15	421.0
16	634.0
17	639.0
18	797.0
19	1046.0
20	1390.0
21	1957.0
22	3196.0
23	5225.0
24	8173.0
25	12599.0
26	18806.0
27	26971.0
28	38324.0
29	51945.0
30	70066.0
31	94485.0
32	129043.0
33	193924.0
34	447288.0
35	1059063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15924026130692	16.739061909256208	11.541256044363385	26.560441785073486
2	16.487619758501456	19.348890660522812	38.068307265372184	26.095182315603548
3	19.219035236998618	23.150036796582764	28.568783755209004	29.062144211209617
4	13.228909155119762	15.653378205261372	35.61584643829071	35.501866201328156
5	14.832618493328697	36.04141594178613	34.08519637211196	15.040769192773215
6	34.31521403442037	35.00881952269786	17.039613298206042	13.636353144675716
7	30.278798756544806	30.183609990111815	20.838999880898633	18.698591372444746
8	27.834515193999493	33.722013999520826	19.357201979303092	19.086268827176596
9	27.647507151868904	14.642623522943454	18.07676700903003	39.63310231615761
10	15.746850145253624	27.13609154417552	32.73554607561313	24.38151223495772
11	36.55690503878027	21.13989656441134	22.848665784314225	19.454532612494166
12	24.73571982215591	23.39136363909154	29.343813288633797	22.52910325011876
13	30.007304136486702	19.946848786830024	24.659263418003448	25.38658365867982
14	23.452599698987083	19.288418389488555	25.564630058817556	31.69435185270681
15	25.004109487837763	27.16782409545094	22.210812108675174	25.617254308036124
16	25.317709261514683	25.845831944427772	24.06492414036364	24.771534653693905
17	24.27353197705961	25.784391539888276	25.268274303982803	24.67380217906932
18	24.370909416588223	25.68871378692518	26.016537533072064	23.92383926341453
19	25.596248275658297	25.06449545807934	25.338543446024183	24.000712820238185
20	25.875052744612663	24.85086085671101	25.113822181637214	24.160264217039117
21	25.91229485946015	24.606061837165456	24.81311828393833	24.668525019436068
22	25.829362225088666	24.650263935196683	25.020521111584067	24.499852728130588
23	25.272897841747877	24.83338042448518	25.042705211236132	24.85101652253081
24	24.85209504699624	24.953714775905645	25.269146846741197	24.925043330356917
25	24.99148145172684	24.658911786623523	25.40254180552593	24.947064956123704
26	24.741864394023693	25.097203413581653	25.58736441041472	24.573567781979936
27	25.08911477096084	25.219630226216015	25.0005193841074	24.69073561871574
28	25.154506578446224	24.876724825249564	25.141718553577437	24.827050042726775
29	24.914323123822996	25.2314995427027	25.047795762393772	24.80638157108053
30	24.816230018202727	25.212784472423305	25.10919096889933	24.861794540474637
31	25.26426685539408	24.710226320549452	24.945304597645432	25.080202226411036
32	24.88063658028304	25.21922150397447	25.086862221696947	24.81327969404554
33	24.84522460783309	25.128044608553296	25.129983629126308	24.896747154487308
34	24.99248593796963	24.914875257336426	25.353991963762603	24.73864684093135
35	25.109390777515987	25.03237745393398	25.381522989261995	24.476708779288035
36	24.76629839840473	25.115205990295962	25.328327261193138	24.790168350106168
37	25.130158378404584	24.73570127630695	25.166815557602323	24.96732478768614
38	24.688053420101603	25.272474280680825	25.113846420901204	24.925625878316364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	93.5
2	147.0
3	147.0
4	350.5
5	554.0
6	554.0
7	655.5
8	757.0
9	733.5
10	710.0
11	710.0
12	1041.5
13	1373.0
14	2014.5
15	2656.0
16	2656.0
17	4352.5
18	6049.0
19	6049.0
20	8507.0
21	10965.0
22	12517.0
23	14069.0
24	14069.0
25	17305.0
26	20541.0
27	20541.0
28	26190.5
29	31840.0
30	40612.5
31	49385.0
32	49385.0
33	61527.0
34	73669.0
35	73669.0
36	84135.0
37	94601.0
38	106452.0
39	118303.0
40	118303.0
41	131044.0
42	143785.0
43	156355.0
44	168925.0
45	168925.0
46	181299.5
47	193674.0
48	193674.0
49	201214.5
50	208755.0
51	210219.5
52	211684.0
53	211684.0
54	207518.0
55	203352.0
56	203352.0
57	192058.5
58	180765.0
59	163104.5
60	145444.0
61	145444.0
62	127494.5
63	109545.0
64	90484.5
65	71424.0
66	71424.0
67	57600.0
68	43776.0
69	43776.0
70	34529.0
71	25282.0
72	19785.0
73	14288.0
74	14288.0
75	11215.5
76	8143.0
77	8143.0
78	6563.5
79	4984.0
80	3907.0
81	2830.0
82	2830.0
83	2168.5
84	1507.0
85	1507.0
86	1130.5
87	754.0
88	583.0
89	412.0
90	412.0
91	295.5
92	179.0
93	163.5
94	148.0
95	148.0
96	133.0
97	118.0
98	118.0
99	454.0
100	790.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12173261940252689
2	0.05728865403054072
3	0.012048621033014607
4	0.0026774713406699123
5	1.8465319590826984E-4
6	2.7697979386240475E-4
7	4.616329897706746E-5
8	2.7697979386240475E-4
9	2.7697979386240475E-4
10	5.539595877248095E-4
11	0.002031185154990968
12	0.0025851447427157775
13	0.01509539876550106
14	0.009371149692344694
15	0.02322013938546493
16	0.011494661445289796
17	0.020127198354001414
18	0.005539595877248095
19	0.008263230516895075
20	0.005770412372133432
21	0.006786004949628916
22	0.007432291135307861
23	0.009740456084161233
24	0.014079806188005575
25	0.016664950930721352
26	0.018649972786735254
27	0.00904800659950522
28	0.006185882062927039
29	0.010894538558587921
30	0.0029544511345323174
31	0.006278208660881174
32	0.0068783315475830515
33	0.00835555711484921
34	0.01311037690948716
35	0.016803440827652557
36	0.014772255672661588
37	0.009601966187230032
38	0.006786004949628916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2166223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.940244185908185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.671252683847655	24.838958999416604
2	15.770903877406054	12.9132931154698
3	7.883831634819306	9.682979767502705
4	4.533322114449324	7.423812573557317
5	2.8984745583237657	5.933212809220867
6	1.946195092685072	4.7806621396765845
7	1.3419826566553446	3.845876835970652
8	0.9654364223367802	3.1620162301149897
9	0.7444976837747029	2.7431925272621456
>10	3.140289189699756	20.569005923912805
>50	0.08115195650175751	2.2253248358671254
>100	0.021869649474118708	1.4321712826672368
>500	4.528457293853784E-4	0.11930242084921712
>1k	3.39634297039032E-4	0.3301905385119731
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3677	0.16974245033867705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3081649488533728E-4
2	0.0	0.0	0.0	0.0	2.3081649488533728E-4
3	0.0	0.0	0.0	0.0	2.3081649488533728E-4
4	0.0	0.0	0.0	0.0	2.3081649488533728E-4
5	0.0	0.0	0.0	0.0	2.3081649488533728E-4
6	0.0	0.0	0.0	0.0	2.7697979386240475E-4
7	0.0	0.0	0.0	0.0	2.7697979386240475E-4
8	0.0	0.0	0.0	0.0	2.7697979386240475E-4
9	0.0	0.0	0.0	4.616329897706746E-5	2.7697979386240475E-4
10	0.0	0.0	0.0	9.232659795413492E-5	2.7697979386240475E-4
11	0.0	0.0	0.0	9.232659795413492E-5	2.7697979386240475E-4
12	0.0	0.0	0.0	9.232659795413492E-5	5.07796288747742E-4
13	0.0	0.0	0.0	9.232659795413492E-5	5.539595877248095E-4
14	0.0	0.0	0.0	9.232659795413492E-5	5.539595877248095E-4
15	0.0	0.0	0.0	1.3848989693120237E-4	7.386127836330794E-4
16	0.0	0.0	0.0	3.231430928394722E-4	7.386127836330794E-4
17	0.0	0.0	0.0	3.231430928394722E-4	7.386127836330794E-4
18	0.0	0.0	0.0	3.693063918165397E-4	9.232659795413491E-4
19	0.0	0.0	0.0	3.693063918165397E-4	9.232659795413491E-4
20	0.0	0.0	0.0	6.00122886701877E-4	0.0010617558764725516
21	0.0	0.0	0.0	6.00122886701877E-4	0.0012464090723808214
22	0.0	0.0	0.0	7.386127836330794E-4	0.0012464090723808214
23	0.0	0.0	0.0	9.232659795413491E-4	0.0012464090723808214
24	0.0	0.0	0.0	0.001523388866243226	0.0012464090723808214
25	0.0	0.0	0.0	0.001615715464197361	0.0012464090723808214
26	0.0	0.0	0.0	0.002400491546807508	0.0012925723713578887
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2170	0.0	21.997728	1
GGTATCA	765	0.0	21.567366	1
GTCCTAA	410	0.0	15.627786	1
TAGGACG	500	0.0	15.036171	4
TATACCG	65	0.004170852	14.76513	5
TATCAAC	3240	0.0	14.47347	2
TCAACGC	3250	0.0	14.174851	4
ATCAACG	3280	0.0	14.095924	3
ACTATAC	230	0.0	13.9114275	3
GTAGGAC	595	0.0	13.712692	3
GTTGTCG	130	1.3378922E-6	13.538451	9
CAACGCA	3405	0.0	13.529281	5
TACTATA	170	1.1439624E-8	13.180417	2
CTTATAC	195	4.2200554E-10	13.126681	3
ACCGTCG	110	5.710393E-5	13.08969	8
AACGCAG	3545	0.0	13.085525	6
GTATTAG	185	2.6448106E-9	12.987957	1
AGGACGT	555	0.0	12.96937	5
GTATAGG	275	0.0	12.814784	1
CTAATCG	100	3.7463297E-4	12.800287	14
>>END_MODULE