FastQCFastQC Report
Thu 2 Feb 2017
SRR4062931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166223
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33870.15635509363532749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC308.418392E-426.6671493
TCGCGTA604.7211215E-621.3347039
GGTATCA7600.021.2645241
GTATCAA23050.020.7562141
CGCGTAA659.436782E-619.6931210
GTATAGG2950.016.8146381
ATACCGT1151.841363E-816.697116
ATAGGAC2500.016.000293
GCGTAAC805.6236753E-516.00028811
CGATCGT859.440585E-515.05839925
TATAGGA2700.014.814742
GTAGCGA650.00416074414.770526
TATCAAC32150.014.6313422
TCAACGC32200.014.4602224
ATCAACG32800.014.244163
GTCGCGT901.5340366E-414.2234648
CAACGCA32900.014.1045745
AATAGCG800.001017784914.0012225
CGACTGA700.006804495613.7148510
TACCGTC1301.336568E-613.5396437
Produced by FastQC (version 0.11.5)