##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166223
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26276103614448	32.0	32.0	32.0	32.0	32.0
2	31.322499114818743	32.0	32.0	32.0	32.0	32.0
3	31.395341569173628	32.0	32.0	32.0	32.0	32.0
4	31.48082353478843	32.0	32.0	32.0	32.0	32.0
5	31.42035515272435	32.0	32.0	32.0	32.0	32.0
6	34.94787055626313	36.0	36.0	36.0	36.0	36.0
7	34.98389500988587	36.0	36.0	36.0	36.0	36.0
8	34.91712072118152	36.0	36.0	36.0	36.0	36.0
9	35.01439463988703	36.0	36.0	36.0	36.0	36.0
10	34.88606020709779	36.0	36.0	36.0	32.0	36.0
11	35.040264091000786	36.0	36.0	36.0	36.0	36.0
12	34.965430613561026	36.0	36.0	36.0	36.0	36.0
13	34.9971295660696	36.0	36.0	36.0	36.0	36.0
14	34.9525293563959	36.0	36.0	36.0	32.0	36.0
15	34.926433705117155	36.0	36.0	36.0	36.0	36.0
16	34.949908204279986	36.0	36.0	36.0	36.0	36.0
17	34.92300284873718	36.0	36.0	36.0	32.0	36.0
18	34.91710179422894	36.0	36.0	36.0	32.0	36.0
19	34.91443817187796	36.0	36.0	36.0	32.0	36.0
20	34.907788810293304	36.0	36.0	36.0	32.0	36.0
21	34.89783461813488	36.0	36.0	36.0	32.0	36.0
22	34.87523445185468	36.0	36.0	36.0	32.0	36.0
23	34.8311249580491	36.0	36.0	36.0	32.0	36.0
24	34.81688034888375	36.0	36.0	36.0	32.0	36.0
25	34.80912768445354	36.0	36.0	36.0	32.0	36.0
26	34.74362288647106	36.0	36.0	36.0	32.0	36.0
27	34.72277969534993	36.0	36.0	36.0	32.0	36.0
28	34.6997335916016	36.0	36.0	36.0	32.0	36.0
29	34.67701755544097	36.0	36.0	36.0	32.0	36.0
30	34.65852638440271	36.0	36.0	36.0	32.0	36.0
31	34.6475108980008	36.0	36.0	36.0	32.0	36.0
32	34.63364898258397	36.0	36.0	36.0	32.0	36.0
33	34.609117343874566	36.0	36.0	36.0	32.0	36.0
34	34.60291622792298	36.0	36.0	36.0	32.0	36.0
35	34.59073834965283	36.0	36.0	36.0	32.0	36.0
36	34.554224565060935	36.0	36.0	36.0	32.0	36.0
37	34.555620543222005	36.0	36.0	36.0	32.0	36.0
38	34.16936852761696	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	14.0
21	45.0
22	143.0
23	414.0
24	1287.0
25	3356.0
26	7074.0
27	13468.0
28	23574.0
29	37322.0
30	56615.0
31	81512.0
32	116800.0
33	185737.0
34	465221.0
35	1173638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.662910496409104	17.846102280848516	12.114987931871863	27.37599929087051
2	15.529617924663308	20.390107754422214	37.144438566314996	26.93583575459948
3	18.289714401518218	23.93437794723812	28.739238757967208	29.036668893276453
4	12.765739398583708	16.54642649407724	35.45346271385178	35.23437139348727
5	14.371980341811689	36.6527068786117	33.7508344020142	15.224478377562411
6	34.31018086805588	35.401251950402084	16.77336558613622	13.51520159540582
7	30.171132755643143	30.283310090005	20.785398296474078	18.76015885787778
8	27.735752720933927	33.953460673410376	19.17561334116283	19.135173264492874
9	27.520759248119813	14.441105688774933	17.875597317443955	40.1625377456613
10	15.494769846952414	27.39820948218276	32.82723714320403	24.27978352766079
11	36.70809463507732	21.13652763113612	22.70271443140148	19.45266330238508
12	24.59589652919955	23.415081143838446	29.345286594359287	22.643735732602718
13	30.02044578174763	19.923904207196234	24.646884407240826	25.408765603815315
14	23.35388524352536	19.24465728812652	25.495817141548745	31.90564032679938
15	25.013398897528095	27.296127868645105	22.074366304854117	25.616106928972687
16	25.23733706086585	25.905458486960946	24.01465592415924	24.842548528013968
17	24.169210649134463	25.89848782881541	25.18097167281485	24.751329849235283
18	24.39324113907017	25.783495051063532	25.82093348653394	24.002330323332362
19	25.56477334051019	25.18351065425859	25.26618912272652	23.98552688250471
20	25.92161564160292	24.93898365957706	24.97425241999554	24.165148278824482
21	25.98671788948686	24.67784926937313	24.661738270592764	24.673694570547248
22	25.818141362307905	24.75786173248251	24.939283664963085	24.484713240246496
23	25.25576834833798	24.884337587445028	24.912497264818224	24.947396799398767
24	24.880033126783346	25.093861527645124	25.146533851777956	24.879571493793577
25	25.073976720760843	24.6378256706838	25.287251260489462	25.000946348065895
26	24.793800084294183	25.18161795900053	25.322369857581606	24.70221209912368
27	25.105460105196975	25.270586301865645	24.879444489068987	24.744509103868396
28	25.157012920645748	24.921810912357593	25.106556434863812	24.814619732132844
29	24.942999866588067	25.15719757384166	24.94701607359907	24.952786485971203
30	24.876478552762112	25.173585544978515	24.997149416288167	24.952786485971203
31	25.18798849425936	24.815312181617497	24.91396315153149	25.082736172591645
32	24.886865295031953	25.198005930137384	24.93838353669036	24.976745238140303
33	24.81891291893771	25.170538767246033	25.09178417919116	24.918764134625103
34	24.988793859173317	25.005320320207108	25.2340133033395	24.771872517280077
35	25.088968217953557	25.096400509088863	25.22376505096659	24.590866221990996
36	24.82943815110448	25.089660667438206	25.21776382209957	24.86313735935774
37	25.043728184956027	24.84988849255132	25.1408096027048	24.96557371978785
38	24.812692327933146	25.19367821026654	24.97606431843089	25.017565143369424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	37.0
2	63.0
3	63.0
4	228.0
5	393.0
6	393.0
7	493.0
8	593.0
9	561.5
10	530.0
11	530.0
12	832.5
13	1135.0
14	1838.5
15	2542.0
16	2542.0
17	4176.5
18	5811.0
19	5811.0
20	8166.0
21	10521.0
22	12284.5
23	14048.0
24	14048.0
25	17368.5
26	20689.0
27	20689.0
28	26383.0
29	32077.0
30	41097.5
31	50118.0
32	50118.0
33	62046.5
34	73975.0
35	73975.0
36	84522.0
37	95069.0
38	107306.0
39	119543.0
40	119543.0
41	131788.5
42	144034.0
43	157005.5
44	169977.0
45	169977.0
46	181772.0
47	193567.0
48	193567.0
49	201300.0
50	209033.0
51	210863.5
52	212694.0
53	212694.0
54	207818.5
55	202943.0
56	202943.0
57	192079.0
58	181215.0
59	163734.0
60	146253.0
61	146253.0
62	127537.0
63	108821.0
64	89689.5
65	70558.0
66	70558.0
67	57015.5
68	43473.0
69	43473.0
70	34263.5
71	25054.0
72	19532.0
73	14010.0
74	14010.0
75	10943.5
76	7877.0
77	7877.0
78	6338.5
79	4800.0
80	3686.5
81	2573.0
82	2573.0
83	1949.5
84	1326.0
85	1326.0
86	929.5
87	533.0
88	397.0
89	261.0
90	261.0
91	158.5
92	56.0
93	44.0
94	32.0
95	32.0
96	21.0
97	10.0
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008632536908711614
2	4.616329897706746E-5
3	0.0
4	1.3848989693120237E-4
5	4.616329897706746E-5
6	1.3848989693120237E-4
7	4.616329897706746E-4
8	0.0025389814437387103
9	0.003693063918165397
10	0.0015695521652202935
11	0.006185882062927039
12	3.693063918165397E-4
13	2.7697979386240475E-4
14	4.616329897706746E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.616329897706746E-5
22	9.232659795413492E-5
23	2.308164948853373E-4
24	0.0
25	4.616329897706746E-5
26	0.0
27	4.616329897706746E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.616329897706746E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2166223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.29077500723396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.668016192223746	21.698622581227305
2	17.191304487535742	13.165367444261653
3	8.604587355256974	9.884289553507054
4	5.062770483148895	7.754296219340779
5	3.1725122887368817	6.07389771278544
6	2.198488566979459	5.0509098644512
7	1.4954311359826131	4.008285201670577
8	1.0842400886258925	3.3213114629917966
9	0.8327931076011856	2.8699464159658965
>10	3.5687155849678014	21.775967375469893
>50	0.09544033919366794	2.452859302440099
>100	0.025094716552271034	1.5751400205819226
>500	2.4226127792815476E-4	0.06038918228058783
>1k	3.633919168922034E-4	0.30871766302578124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3387	0.15635509363532749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.616329897706746E-5
2	0.0	0.0	0.0	0.0	4.616329897706746E-5
3	0.0	0.0	0.0	0.0	4.616329897706746E-5
4	0.0	0.0	0.0	0.0	4.616329897706746E-5
5	0.0	0.0	0.0	0.0	4.616329897706746E-5
6	0.0	0.0	0.0	0.0	2.3081649488533728E-4
7	0.0	0.0	0.0	0.0	2.3081649488533728E-4
8	0.0	0.0	0.0	0.0	2.3081649488533728E-4
9	0.0	0.0	0.0	0.0	2.3081649488533728E-4
10	0.0	0.0	0.0	4.616329897706746E-5	2.7697979386240475E-4
11	0.0	0.0	0.0	4.616329897706746E-5	2.7697979386240475E-4
12	0.0	0.0	0.0	4.616329897706746E-5	6.924494846560119E-4
13	0.0	0.0	0.0	4.616329897706746E-5	7.847760826101467E-4
14	0.0	0.0	0.0	9.232659795413492E-5	7.847760826101467E-4
15	0.0	0.0	0.0	1.3848989693120237E-4	0.001015592577495484
16	0.0	0.0	0.0	3.231430928394722E-4	0.001200245773403754
17	0.0	0.0	0.0	3.231430928394722E-4	0.001200245773403754
18	0.0	0.0	0.0	3.693063918165397E-4	0.0012464090723808214
19	0.0	0.0	0.0	3.693063918165397E-4	0.0012464090723808214
20	0.0	0.0	0.0	4.6163298977067456E-4	0.0013848989693120237
21	0.0	0.0	0.0	4.6163298977067456E-4	0.0013848989693120237
22	0.0	0.0	0.0	6.00122886701877E-4	0.0013848989693120237
23	0.0	0.0	0.0	7.386127836330794E-4	0.0013848989693120237
24	0.0	0.0	0.0	0.0012464090723808214	0.0013848989693120237
25	0.0	0.0	0.0	0.0012925723713578887	0.0013848989693120237
26	0.0	0.0	0.0	0.0019388585570368332	0.001523388866243226
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	30	8.418392E-4	26.667149	3
TCGCGTA	60	4.7211215E-6	21.334703	9
GGTATCA	760	0.0	21.264524	1
GTATCAA	2305	0.0	20.756214	1
CGCGTAA	65	9.436782E-6	19.69312	10
GTATAGG	295	0.0	16.814638	1
ATACCGT	115	1.841363E-8	16.69711	6
ATAGGAC	250	0.0	16.00029	3
GCGTAAC	80	5.6236753E-5	16.000288	11
CGATCGT	85	9.440585E-5	15.058399	25
TATAGGA	270	0.0	14.81474	2
GTAGCGA	65	0.004160744	14.77052	6
TATCAAC	3215	0.0	14.631342	2
TCAACGC	3220	0.0	14.460222	4
ATCAACG	3280	0.0	14.24416	3
GTCGCGT	90	1.5340366E-4	14.223464	8
CAACGCA	3290	0.0	14.104574	5
AATAGCG	80	0.0010177849	14.001222	5
CGACTGA	70	0.0068044956	13.71485	10
TACCGTC	130	1.336568E-6	13.539643	7
>>END_MODULE