FastQCFastQC Report
Thu 2 Feb 2017
SRR4062930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798628
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41630.5212689762943449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27130.33970759853148147No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15810.19796450913316335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15150.18970033607637096No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13440.1682886149746816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.16277916627015332No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11490.1438717400341586No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10890.1363588554370746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.1363588554370746No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10840.13573278172065092No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10520.13172590993553945No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10460.13097462147583103No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10290.12884597083999058No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9950.12458866956830965No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9700.12145830098619131No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9550.11958007983692032No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9400.1177018586876493No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8960.11219240998312106No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8690.10881161191443325No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8070.10104829783077979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2500.020.47951928
ACGTTTT951.720764E-920.21131529
CTATTAG400.00446020820.0170821
CTAGCGG2600.019.69307929
CTAGACA659.455347E-619.6856824
GGCATCG2200.018.90864826
TAGCGGC2600.018.46226130
ACCGTCG1950.018.0508588
CACGTTT904.3636828E-717.7773628
TACCGTC1800.017.777367
CGGCATC2500.017.27959425
ACTATAC652.1017785E-417.2282073
ATCGTTT2350.017.02194229
ATACCGT2000.016.7943466
CGTTTTT1051.1871998E-716.76256230
GCGCAAG2400.016.6809021
CAAGACG2250.016.3500524
CATCGTT2450.016.32614728
AGCGGCG2950.016.27182431
CGCAAGA2300.016.0026322
Produced by FastQC (version 0.11.5)