##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062930_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	798628
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16101864697957	32.0	32.0	32.0	32.0	32.0
2	30.79619672738747	32.0	32.0	32.0	32.0	32.0
3	30.87071076896878	32.0	32.0	32.0	32.0	32.0
4	30.92041601346309	32.0	32.0	32.0	32.0	32.0
5	30.882281863395722	32.0	32.0	32.0	32.0	32.0
6	34.50790480674357	36.0	36.0	36.0	32.0	36.0
7	34.39061740885619	36.0	36.0	36.0	32.0	36.0
8	34.38120000801374	36.0	36.0	36.0	32.0	36.0
9	34.486923824358776	36.0	36.0	36.0	32.0	36.0
10	34.25382155396505	36.0	36.0	36.0	32.0	36.0
11	34.52423030497303	36.0	36.0	36.0	32.0	36.0
12	34.34014209369068	36.0	36.0	36.0	32.0	36.0
13	34.421320063909604	36.0	36.0	36.0	32.0	36.0
14	34.321654136844685	36.0	36.0	36.0	32.0	36.0
15	34.28588779757284	36.0	36.0	36.0	32.0	36.0
16	34.29955749109723	36.0	36.0	36.0	32.0	36.0
17	34.219183399530195	36.0	36.0	36.0	32.0	36.0
18	34.248532483208706	36.0	36.0	36.0	32.0	36.0
19	34.22794342297039	36.0	36.0	36.0	32.0	36.0
20	34.18100041571295	36.0	36.0	36.0	32.0	36.0
21	34.16370951181276	36.0	36.0	36.0	32.0	36.0
22	34.14020044376105	36.0	36.0	36.0	32.0	36.0
23	34.106227179613036	36.0	36.0	36.0	32.0	36.0
24	34.10606440044677	36.0	36.0	36.0	32.0	36.0
25	34.07467556860015	36.0	36.0	36.0	32.0	36.0
26	34.039475199967946	36.0	36.0	36.0	32.0	36.0
27	34.02281913481621	36.0	36.0	36.0	32.0	36.0
28	34.022637573438445	36.0	36.0	36.0	32.0	36.0
29	33.984116509814335	36.0	36.0	36.0	32.0	36.0
30	33.933249272502344	36.0	36.0	36.0	32.0	36.0
31	33.96072013503158	36.0	36.0	36.0	32.0	36.0
32	33.91593082135863	36.0	36.0	36.0	32.0	36.0
33	33.86827659435933	36.0	36.0	36.0	32.0	36.0
34	33.8453197233255	36.0	36.0	36.0	32.0	36.0
35	33.79934713032851	36.0	36.0	36.0	32.0	36.0
36	33.75802125645482	36.0	36.0	36.0	32.0	36.0
37	33.75268836053832	36.0	36.0	36.0	27.0	36.0
38	33.14576248265776	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	1.0
9	0.0
10	7.0
11	9.0
12	3.0
13	3.0
14	200.0
15	377.0
16	417.0
17	438.0
18	635.0
19	764.0
20	955.0
21	1342.0
22	1946.0
23	2835.0
24	4014.0
25	5660.0
26	8121.0
27	11332.0
28	16113.0
29	21441.0
30	28472.0
31	38053.0
32	50901.0
33	75358.0
34	167906.0
35	361321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.860345344197064	17.801787525434438	11.775627821608522	24.562239308759978
2	15.938503015466953	20.630078499858435	38.25897185981774	25.17244662485687
3	18.47834390038958	25.566432200322836	28.963334840229365	26.99188905905822
4	12.141174761743171	16.11306101954263	36.89459099714129	34.8511732215729
5	13.923270813533728	37.45803735661329	33.49085367762422	15.127838152228762
6	32.24450084708304	36.833775135451894	17.613316921751565	13.308407095713498
7	29.069677170540402	31.73325186006967	21.23396934234548	17.96310162704445
8	27.954777103560886	32.63058117607579	20.029125168102055	19.38551655226127
9	27.84890010818608	14.57907601073847	19.25161277397123	38.32041110710422
10	16.016944165144253	26.6731611786788	31.395163925907465	25.914730730269476
11	36.274697630505045	22.042326094496804	22.82994556785611	18.853030707142043
12	24.797115985419648	24.834806519836366	28.743853375055878	21.624224119688108
13	29.1472087702359	20.00641186997429	25.885545616322215	24.960833743467596
14	23.69532930772371	20.771795019497745	25.712850978142992	29.820024694635556
15	24.88677581611591	27.50107713272279	23.644816737139536	23.967330314021762
16	24.934505472487288	26.578130087409523	24.692689157712827	23.79467528239036
17	23.083734427465526	26.329684973592286	26.193669914245387	24.392910684696805
18	24.12760666133219	25.069780197075055	27.814781004759702	22.98783213683305
19	24.84359425168177	25.646159856943214	26.71871008460186	22.791535806773158
20	25.048523750967345	24.481766339668763	26.550443413166903	23.91926649619699
21	26.787865430803254	24.489029249490972	25.127664423512797	23.595440896192983
22	25.169554350058355	25.0276746760435	25.836125845893378	23.966645128004767
23	23.378056171952093	24.82280338814949	26.708350656441443	25.090789783456973
24	23.718860675159174	26.14200483902478	26.30055052673236	23.838583959083692
25	24.072381076423678	25.24533623211638	26.397627539774778	24.28465515168517
26	23.433367064764347	26.16097907983328	27.176178262263544	23.229475593138826
27	24.449787428542805	25.77943911189586	25.994828158361038	23.775945301200295
28	23.30120551327918	25.380268250809873	27.18346667401303	24.13505956189792
29	23.203124706495238	25.824912746904033	26.970630113244315	24.001332433356417
30	23.58167240379715	25.925401860007334	27.138902002131232	23.35402373406428
31	23.993748927788346	25.702650820265394	25.736210262427324	24.567389989518933
32	23.349457969297656	26.21532712808254	25.787187363427922	24.64802753919188
33	23.23177932195197	25.49298296802858	26.588458932800034	24.68677877721941
34	23.98964600041075	25.81086293348294	26.864546442722396	23.33494462338391
35	24.915872986055014	25.634099149024102	26.377622903086433	23.07240496183445
36	23.382120703970823	26.517378230905734	26.04110963194694	24.059391433176497
37	24.580897149962873	26.414718659171427	25.665236158992975	23.339148031872732
38	23.49889428459086	26.167637976503233	26.226617813162896	24.106849925743006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1248.0
1	857.5
2	467.0
3	467.0
4	884.5
5	1302.0
6	1302.0
7	1493.5
8	1685.0
9	1585.0
10	1485.0
11	1485.0
12	1877.0
13	2269.0
14	2754.0
15	3239.0
16	3239.0
17	4677.0
18	6115.0
19	6115.0
20	7174.5
21	8234.0
22	8242.0
23	8250.0
24	8250.0
25	9323.5
26	10397.0
27	10397.0
28	13686.0
29	16975.0
30	20358.0
31	23741.0
32	23741.0
33	28685.5
34	33630.0
35	33630.0
36	36483.0
37	39336.0
38	43684.0
39	48032.0
40	48032.0
41	50080.5
42	52129.0
43	57902.0
44	63675.0
45	63675.0
46	66112.5
47	68550.0
48	68550.0
49	69474.5
50	70399.0
51	68749.0
52	67099.0
53	67099.0
54	62927.0
55	58755.0
56	58755.0
57	56752.0
58	54749.0
59	48780.5
60	42812.0
61	42812.0
62	41386.5
63	39961.0
64	33003.5
65	26046.0
66	26046.0
67	21960.0
68	17874.0
69	17874.0
70	14572.5
71	11271.0
72	8890.0
73	6509.0
74	6509.0
75	4972.0
76	3435.0
77	3435.0
78	3321.5
79	3208.0
80	2540.0
81	1872.0
82	1872.0
83	1901.5
84	1931.0
85	1931.0
86	1276.0
87	621.0
88	549.5
89	478.0
90	478.0
91	349.0
92	220.0
93	182.5
94	145.0
95	145.0
96	136.5
97	128.0
98	128.0
99	242.0
100	356.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12383738110860125
2	0.051338044746740655
3	0.009641535232924466
4	0.0018782211492709997
5	1.252147432847333E-4
6	6.260737164236666E-4
7	2.504294865694666E-4
8	7.512884597084E-4
9	5.008589731389332E-4
10	6.260737164236666E-4
11	0.002379080122409933
12	0.002379080122409933
13	0.013648407018035933
14	0.009265891003070266
15	0.02654552557636346
16	0.011018897409056533
17	0.02341515699424513
18	0.006135522420951933
19	0.008013743570222932
20	0.006260737164236666
21	0.006260737164236666
22	0.008013743570222932
23	0.010017179462778665
24	0.015025769194167997
25	0.016528346113584797
26	0.020535217898696265
27	0.009140676259785532
28	0.005885092934382466
29	0.011144112152341266
30	0.0036312275552572663
31	0.005634663447812999
32	0.007137240367229799
33	0.008890246773216065
34	0.0120206153553344
35	0.01665356085686953
36	0.016152701883730598
37	0.009641535232924466
38	0.006260737164236666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	798628.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83533484188015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.99680272038424	44.14325330415475
2	11.375825569063643	12.248427572267259
3	2.965180611887953	4.788944733229169
4	1.1598644271757959	2.497667592327779
5	0.5867908136904174	1.5795039968581466
6	0.34454339321379646	1.1129165364733387
7	0.2396559365573682	0.9031370313987211
8	0.16597238388350968	0.7148143088699052
9	0.11984640525536873	0.5806774220866596
>10	0.7975401168850924	8.511503543169791
>50	0.11549029800541591	4.38079761317936
>100	0.12084520413882696	12.85291442989574
>500	0.00861516869538085	3.1591081040939697
>1k	0.0030269511632419204	2.5263338119954715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4163	0.5212689762943449	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2713	0.33970759853148147	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1581	0.19796450913316335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1515	0.18970033607637096	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1344	0.1682886149746816	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.16277916627015332	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1149	0.1438717400341586	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1089	0.1363588554370746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1089	0.1363588554370746	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1084	0.13573278172065092	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1052	0.13172590993553945	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1046	0.13097462147583103	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1029	0.12884597083999058	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	995	0.12458866956830965	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	970	0.12145830098619131	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	955	0.11958007983692032	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	940	0.1177018586876493	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	896	0.11219240998312106	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	869	0.10881161191443325	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	807	0.10104829783077979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.252147432847333E-4
2	0.0	0.0	0.0	0.0	1.252147432847333E-4
3	0.0	0.0	0.0	0.0	1.252147432847333E-4
4	0.0	0.0	0.0	0.0	1.252147432847333E-4
5	0.0	0.0	0.0	1.252147432847333E-4	1.252147432847333E-4
6	0.0	0.0	0.0	1.252147432847333E-4	2.504294865694666E-4
7	0.0	0.0	0.0	1.252147432847333E-4	2.504294865694666E-4
8	0.0	0.0	0.0	1.252147432847333E-4	2.504294865694666E-4
9	0.0	0.0	0.0	3.756442298542E-4	2.504294865694666E-4
10	0.0	0.0	0.0	3.756442298542E-4	2.504294865694666E-4
11	0.0	0.0	0.0	3.756442298542E-4	2.504294865694666E-4
12	0.0	0.0	0.0	3.756442298542E-4	7.512884597084E-4
13	0.0	0.0	0.0	3.756442298542E-4	7.512884597084E-4
14	0.0	0.0	0.0	3.756442298542E-4	7.512884597084E-4
15	0.0	0.0	0.0	3.756442298542E-4	7.512884597084E-4
16	0.0	0.0	0.0	3.756442298542E-4	7.512884597084E-4
17	0.0	0.0	1.252147432847333E-4	5.008589731389332E-4	8.765032029931332E-4
18	0.0	0.0	1.252147432847333E-4	6.260737164236666E-4	0.0010017179462778665
19	0.0	0.0	1.252147432847333E-4	6.260737164236666E-4	0.0010017179462778665
20	0.0	0.0	1.252147432847333E-4	0.0010017179462778665	0.0010017179462778665
21	0.0	0.0	1.252147432847333E-4	0.0016277916627015331	0.0011269326895626
22	0.0	0.0	1.252147432847333E-4	0.0030051538388336	0.0011269326895626
23	0.0	0.0	1.252147432847333E-4	0.004758160244819866	0.0011269326895626
24	0.0	0.0	1.252147432847333E-4	0.008264173056792399	0.0011269326895626
25	0.0	0.0	1.252147432847333E-4	0.010392823692632865	0.0011269326895626
26	0.0	0.0	1.252147432847333E-4	0.012646689071758066	0.0011269326895626
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	250	0.0	20.479519	28
ACGTTTT	95	1.720764E-9	20.211315	29
CTATTAG	40	0.004460208	20.017082	1
CTAGCGG	260	0.0	19.693079	29
CTAGACA	65	9.455347E-6	19.685682	4
GGCATCG	220	0.0	18.908648	26
TAGCGGC	260	0.0	18.462261	30
ACCGTCG	195	0.0	18.050858	8
CACGTTT	90	4.3636828E-7	17.77736	28
TACCGTC	180	0.0	17.77736	7
CGGCATC	250	0.0	17.279594	25
ACTATAC	65	2.1017785E-4	17.228207	3
ATCGTTT	235	0.0	17.021942	29
ATACCGT	200	0.0	16.794346	6
CGTTTTT	105	1.1871998E-7	16.762562	30
GCGCAAG	240	0.0	16.680902	1
CAAGACG	225	0.0	16.350052	4
CATCGTT	245	0.0	16.326147	28
AGCGGCG	295	0.0	16.271824	31
CGCAAGA	230	0.0	16.002632	2
>>END_MODULE