FastQCFastQC Report
Thu 2 Feb 2017
SRR4062930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798628
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50610.6337118157640353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36950.46266847643708964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21690.2715907781845866No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17010.21299027832733136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.20760604436608782No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15570.19495935529432978No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12020.15050812142824943No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11620.1454995316968601No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11060.13848750607291505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11060.13848750607291505No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10770.13485627851765777No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10060.1259660317444417No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9650.12083222726976764No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9650.12083222726976764No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9350.11707578497122564No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9240.1156984227950936No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9160.11469670484881571No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8700.10893682665771799No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8460.1059316728188844No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8290.10380302218304391No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8200.10267608949348131No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8100.10142394206063399No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.10054743885764086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC400.00448286219.9995693
TCTTATA751.5077003E-619.1995872
ATCGTTT2000.018.39960329
CATTCCG450.008845882517.778519
CATCGTT2100.017.52343228
ACGATTA550.001364347917.4541732
CGATGCG1201.6570993E-917.3329622
GTATATA652.0957222E-417.2347151
TCAACGA854.9811097E-616.94081129
TAATACT1800.016.8885254
TACTTGT1407.403287E-1016.0006587
AGGCCCG3000.016.00065810
TATACTG908.5708925E-616.0006585
TACAGCG600.002440360816.0006585
GCAACGC600.002441380915.99965620
TAGGACG1203.10647E-815.9996564
GGCATCG2350.015.65923826
GCATCGT2350.015.65923827
ATACTTG1551.8735591E-1015.4845086
CGGCATC2400.015.33300325
Produced by FastQC (version 0.11.5)