##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	798628
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.111253299408485	32.0	32.0	32.0	32.0	32.0
2	31.304606149546473	32.0	32.0	32.0	32.0	32.0
3	31.378829692923365	32.0	32.0	32.0	32.0	32.0
4	31.480693138732928	32.0	32.0	32.0	32.0	32.0
5	31.418492715006234	32.0	32.0	32.0	32.0	32.0
6	34.91054408310252	36.0	36.0	36.0	36.0	36.0
7	34.937621771337845	36.0	36.0	36.0	36.0	36.0
8	34.87452981863897	36.0	36.0	36.0	32.0	36.0
9	34.986409191763876	36.0	36.0	36.0	36.0	36.0
10	34.83680136433984	36.0	36.0	36.0	32.0	36.0
11	35.00518389037199	36.0	36.0	36.0	36.0	36.0
12	34.90696669788687	36.0	36.0	36.0	32.0	36.0
13	34.94247759908242	36.0	36.0	36.0	36.0	36.0
14	34.887721191844015	36.0	36.0	36.0	32.0	36.0
15	34.87227219681754	36.0	36.0	36.0	32.0	36.0
16	34.87960226788943	36.0	36.0	36.0	32.0	36.0
17	34.84650550694441	36.0	36.0	36.0	32.0	36.0
18	34.82955268285109	36.0	36.0	36.0	32.0	36.0
19	34.82610426882103	36.0	36.0	36.0	32.0	36.0
20	34.815648837756754	36.0	36.0	36.0	32.0	36.0
21	34.79724352264133	36.0	36.0	36.0	32.0	36.0
22	34.77609099605824	36.0	36.0	36.0	32.0	36.0
23	34.711292366408394	36.0	36.0	36.0	32.0	36.0
24	34.70476116539866	36.0	36.0	36.0	32.0	36.0
25	34.686943107429244	36.0	36.0	36.0	32.0	36.0
26	34.619781675573606	36.0	36.0	36.0	32.0	36.0
27	34.599188858893	36.0	36.0	36.0	32.0	36.0
28	34.56444552407379	36.0	36.0	36.0	32.0	36.0
29	34.519690018381525	36.0	36.0	36.0	32.0	36.0
30	34.48684243477564	36.0	36.0	36.0	32.0	36.0
31	34.47655353931994	36.0	36.0	36.0	32.0	36.0
32	34.438648532232776	36.0	36.0	36.0	32.0	36.0
33	34.37345798043645	36.0	36.0	36.0	32.0	36.0
34	34.35508396900685	36.0	36.0	36.0	32.0	36.0
35	34.29321536434986	36.0	36.0	36.0	32.0	36.0
36	34.25708840661735	36.0	36.0	36.0	32.0	36.0
37	34.25118578361891	36.0	36.0	36.0	32.0	36.0
38	33.83380497553304	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	7.0
21	32.0
22	86.0
23	200.0
24	590.0
25	1479.0
26	3084.0
27	5916.0
28	10287.0
29	15851.0
30	23586.0
31	33285.0
32	47087.0
33	73507.0
34	174972.0
35	408654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82066598292653	19.116672865409637	12.551640538025843	25.511020613637985
2	15.170141793175295	21.749174834841753	37.44559419404278	25.635089177940166
3	17.67356015566697	26.087364830684624	29.344450733007104	26.8946242806413
4	11.628250591016547	16.84672636935529	36.86929017910806	34.6557328605201
5	13.344527545399792	37.96941500850835	33.26721986609519	15.41883757999667
6	32.266461982299646	37.113274265365106	17.17044731714891	13.449816435186346
7	28.684462275113194	31.900619064791442	21.231267780582602	18.18365087951276
8	27.82964528634538	32.76505855129374	20.073226682160136	19.332069480200747
9	27.680030453255128	14.182050861443951	19.235387884563128	38.902530800737786
10	15.745015470417131	26.886720985298407	31.515825404040214	25.852438140244242
11	36.56725762785724	21.78951296416416	22.7549694084294	18.888259999549202
12	24.500891533437514	24.87503506030372	28.90196137356253	21.722112032696238
13	29.16358846318552	19.988830816928076	25.950194458983304	24.8973862609031
14	23.737614681196604	20.528982866845222	25.81618202239594	29.917220429562235
15	24.757584257000754	27.495154189434878	23.731449435782366	24.015812117781994
16	24.951667109092092	26.316758240382253	24.76321892044857	23.968355730077082
17	22.96488477739323	26.38687849662171	26.10439403577135	24.543842690213715
18	24.154424838598196	24.668180930295456	28.013292797147106	23.16410143395924
19	24.943903795008442	25.434870803427877	26.872085626850044	22.749139774713633
20	25.127092964434006	24.29641835748308	26.758014995717655	23.81847368236526
21	26.634210964567938	24.242606373187982	25.410861390862067	23.71232127138201
22	24.897261045846243	24.91291292795376	26.220408551687523	23.969417474512476
23	23.332949340492295	24.44147823887527	26.936137816700185	25.289434603932254
24	23.747226493436244	25.833178901816616	26.58960116599969	23.82999343874745
25	24.118989066248616	24.676319888608962	26.819620649413743	24.385070395728675
26	23.512448849777368	25.72511857836189	27.454960256840483	23.30747231502026
27	24.564628337598982	25.341210175450897	26.228356631623235	23.865804855326886
28	23.3022132958023	24.950915820632385	27.60446666032245	24.14240422324286
29	23.32788231817567	25.260071021802393	27.415141968475936	23.996904691546
30	23.54024652278658	25.355484656185357	27.56364665401163	23.540622167016434
31	23.986386653110085	25.22163009561398	26.2803207500864	24.51166250118954
32	23.42868018651988	25.709216305964727	26.324271124979337	24.53783238253605
33	23.293573478515654	24.944529868724864	27.01232613932895	24.749570513430534
34	23.868183935449295	25.27747587111897	27.31659796551085	23.537742227920884
35	25.09591449335611	25.0443260191228	26.82137365581973	23.03838583170137
36	23.503057744031015	25.839063994751	26.50683422068848	24.151044040529506
37	24.60945521569492	25.871369398518457	26.036903289140877	23.482272096645747
38	23.70239961534031	25.467551851425196	26.729591248992023	24.100457284242477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	706.0
1	488.0
2	270.0
3	270.0
4	816.5
5	1363.0
6	1363.0
7	1531.0
8	1699.0
9	1661.5
10	1624.0
11	1624.0
12	2020.5
13	2417.0
14	3025.5
15	3634.0
16	3634.0
17	5268.5
18	6903.0
19	6903.0
20	7834.5
21	8766.0
22	8681.5
23	8597.0
24	8597.0
25	9439.5
26	10282.0
27	10282.0
28	13395.0
29	16508.0
30	19944.5
31	23381.0
32	23381.0
33	28449.0
34	33517.0
35	33517.0
36	36248.0
37	38979.0
38	43279.5
39	47580.0
40	47580.0
41	49439.5
42	51299.0
43	57409.0
44	63519.0
45	63519.0
46	66279.0
47	69039.0
48	69039.0
49	70094.0
50	71149.0
51	69045.5
52	66942.0
53	66942.0
54	62874.0
55	58806.0
56	58806.0
57	57032.5
58	55259.0
59	48990.5
60	42722.0
61	42722.0
62	41582.5
63	40443.0
64	33358.5
65	26274.0
66	26274.0
67	22104.5
68	17935.0
69	17935.0
70	14662.5
71	11390.0
72	9027.5
73	6665.0
74	6665.0
75	4954.5
76	3244.0
77	3244.0
78	3312.0
79	3380.0
80	2560.5
81	1741.0
82	1741.0
83	1750.5
84	1760.0
85	1760.0
86	1100.5
87	441.0
88	375.5
89	310.0
90	310.0
91	181.5
92	53.0
93	34.5
94	16.0
95	16.0
96	11.0
97	6.0
98	6.0
99	7.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009891964719493931
2	0.0
3	0.0
4	5.008589731389332E-4
5	1.252147432847333E-4
6	0.0
7	5.008589731389332E-4
8	0.0015025769194168
9	0.0033807980686877993
10	8.765032029931333E-4
11	0.0052590192179588
12	5.008589731389332E-4
13	2.504294865694666E-4
14	1.252147432847333E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.252147432847333E-4
22	2.504294865694666E-4
23	2.504294865694666E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	798628.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.65137138656788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76043508143587	41.7138722573205
2	12.063068432602392	12.461480553478589
3	3.1859988453982067	4.9368362880251775
4	1.2648844258407748	2.6133206096075017
5	0.6399628029689279	1.6527478204868538
6	0.40100095219108506	1.2427349464793453
7	0.25624160848001715	0.926466133900502
8	0.1802802080195274	0.7449375982451459
9	0.13742206402743537	0.6388234259210798
>10	0.845551122227183	8.654554972352207
>50	0.12393371010331361	4.589850235453537
>100	0.12782912417310066	13.337653175985956
>500	0.010469813979708224	3.65705788278568
>1k	0.0026783245064369876	2.193178321740757
>5k	2.4348404603972615E-4	0.6364857782172127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5061	0.6337118157640353	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3695	0.46266847643708964	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2169	0.2715907781845866	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1701	0.21299027832733136	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1658	0.20760604436608782	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1557	0.19495935529432978	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1202	0.15050812142824943	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1162	0.1454995316968601	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1106	0.13848750607291505	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1106	0.13848750607291505	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1077	0.13485627851765777	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1006	0.1259660317444417	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	965	0.12083222726976764	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	965	0.12083222726976764	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	935	0.11707578497122564	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	924	0.1156984227950936	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	916	0.11469670484881571	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	870	0.10893682665771799	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	846	0.1059316728188844	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	829	0.10380302218304391	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	820	0.10267608949348131	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	810	0.10142394206063399	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.10054743885764086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.252147432847333E-4	0.0
10	0.0	0.0	0.0	2.504294865694666E-4	0.0
11	0.0	0.0	0.0	2.504294865694666E-4	0.0
12	0.0	0.0	0.0	2.504294865694666E-4	1.252147432847333E-4
13	0.0	0.0	0.0	2.504294865694666E-4	2.504294865694666E-4
14	0.0	0.0	0.0	2.504294865694666E-4	2.504294865694666E-4
15	0.0	0.0	0.0	2.504294865694666E-4	5.008589731389332E-4
16	0.0	0.0	0.0	2.504294865694666E-4	6.260737164236666E-4
17	0.0	0.0	0.0	3.756442298542E-4	6.260737164236666E-4
18	0.0	0.0	0.0	3.756442298542E-4	6.260737164236666E-4
19	0.0	0.0	0.0	5.008589731389332E-4	6.260737164236666E-4
20	0.0	0.0	0.0	8.765032029931332E-4	8.765032029931332E-4
21	0.0	0.0	0.0	0.0015025769194168	8.765032029931332E-4
22	0.0	0.0	1.252147432847333E-4	0.0030051538388336	8.765032029931332E-4
23	0.0	0.0	1.252147432847333E-4	0.004758160244819866	8.765032029931332E-4
24	0.0	0.0	1.252147432847333E-4	0.008138958313507666	8.765032029931332E-4
25	0.0	0.0	1.252147432847333E-4	0.010643253179202332	8.765032029931332E-4
26	0.0	0.0	1.252147432847333E-4	0.01289711855832753	8.765032029931332E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	40	0.004482862	19.999569	3
TCTTATA	75	1.5077003E-6	19.199587	2
ATCGTTT	200	0.0	18.399603	29
CATTCCG	45	0.0088458825	17.77851	9
CATCGTT	210	0.0	17.523432	28
ACGATTA	55	0.0013643479	17.45417	32
CGATGCG	120	1.6570993E-9	17.33296	22
GTATATA	65	2.0957222E-4	17.234715	1
TCAACGA	85	4.9811097E-6	16.940811	29
TAATACT	180	0.0	16.888525	4
TACTTGT	140	7.403287E-10	16.000658	7
AGGCCCG	300	0.0	16.000658	10
TATACTG	90	8.5708925E-6	16.000658	5
TACAGCG	60	0.0024403608	16.000658	5
GCAACGC	60	0.0024413809	15.999656	20
TAGGACG	120	3.10647E-8	15.999656	4
GGCATCG	235	0.0	15.659238	26
GCATCGT	235	0.0	15.659238	27
ATACTTG	155	1.8735591E-10	15.484508	6
CGGCATC	240	0.0	15.333003	25
>>END_MODULE