FastQCFastQC Report
Thu 2 Feb 2017
SRR4062929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425790
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62100.2559990765894822No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT37600.1550010512039377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36930.15223906438727178No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC35610.14679753812160162No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31580.1301843935377753No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30020.12375349886016515No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29000.11954868310942002No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28750.11851809101364917No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28550.11769361733703247No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27260.11237576212285481No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT26790.1104382489828056No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25590.10549140692310546No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25270.10417224904051875No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG24880.10256452537111621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2550.017.5897161
TAGGACG2500.017.2775634
AAGACGG7900.017.0099075
CGCTTCG5900.016.54174432
TAAACGC6050.016.39573328
CAAGACG8250.016.0946984
ATCGTTT6450.015.87536629
TCTAGCG7100.015.77372328
CGTTTAT6550.015.63299431
AAACGCT6250.015.61540629
GTATCAA53250.015.5830021
AACGCTT6300.015.49147530
GTAAACG6450.015.37830927
CATCGTT6900.015.3034528
TCGTTTA6650.015.15731730
CTAGCGG7400.015.1345629
TATAGGG1807.2759576E-1215.1167712
ACGCTTC6500.015.01481331
CGGCATC7050.014.97784625
AATCGGG756.2506757E-414.93245725
Produced by FastQC (version 0.11.5)