##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062929_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2425790
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2618672679828	32.0	32.0	32.0	32.0	32.0
2	30.825120888452833	32.0	32.0	32.0	32.0	32.0
3	30.862554054555424	32.0	32.0	32.0	32.0	32.0
4	30.94180699895704	32.0	32.0	32.0	32.0	32.0
5	30.82652826501882	32.0	32.0	32.0	32.0	32.0
6	34.50708387782949	36.0	36.0	36.0	32.0	36.0
7	34.4345837850762	36.0	36.0	36.0	32.0	36.0
8	34.40594734086628	36.0	36.0	36.0	32.0	36.0
9	34.57849484085597	36.0	36.0	36.0	32.0	36.0
10	34.25518449659698	36.0	36.0	36.0	32.0	36.0
11	34.55703915013253	36.0	36.0	36.0	32.0	36.0
12	34.35577193409157	36.0	36.0	36.0	32.0	36.0
13	34.45415926358011	36.0	36.0	36.0	32.0	36.0
14	34.34063665857308	36.0	36.0	36.0	32.0	36.0
15	34.27608449206238	36.0	36.0	36.0	32.0	36.0
16	34.301336884066636	36.0	36.0	36.0	32.0	36.0
17	34.20918504899435	36.0	36.0	36.0	32.0	36.0
18	34.248655901788695	36.0	36.0	36.0	32.0	36.0
19	34.236462348348375	36.0	36.0	36.0	32.0	36.0
20	34.22545851042341	36.0	36.0	36.0	32.0	36.0
21	34.20675862296407	36.0	36.0	36.0	32.0	36.0
22	34.18358019449334	36.0	36.0	36.0	32.0	36.0
23	34.129887583014195	36.0	36.0	36.0	32.0	36.0
24	34.10559776402739	36.0	36.0	36.0	32.0	36.0
25	34.08397305620025	36.0	36.0	36.0	32.0	36.0
26	34.03863937109148	36.0	36.0	36.0	32.0	36.0
27	34.04142114527638	36.0	36.0	36.0	32.0	36.0
28	34.02268168308056	36.0	36.0	36.0	32.0	36.0
29	33.99726151068312	36.0	36.0	36.0	32.0	36.0
30	33.97170447565535	36.0	36.0	36.0	32.0	36.0
31	33.98614513210129	36.0	36.0	36.0	32.0	36.0
32	33.93285156588163	36.0	36.0	36.0	32.0	36.0
33	33.90589869691935	36.0	36.0	36.0	32.0	36.0
34	33.91435779684144	36.0	36.0	36.0	32.0	36.0
35	33.876474880348255	36.0	36.0	36.0	32.0	36.0
36	33.8356663190136	36.0	36.0	36.0	32.0	36.0
37	33.828854105260554	36.0	36.0	36.0	32.0	36.0
38	33.24505789866394	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	2.0
9	12.0
10	18.0
11	21.0
12	12.0
13	9.0
14	205.0
15	480.0
16	664.0
17	798.0
18	1008.0
19	1357.0
20	1928.0
21	3000.0
22	4561.0
23	7086.0
24	10872.0
25	17042.0
26	24686.0
27	35129.0
28	49113.0
29	65717.0
30	88086.0
31	118285.0
32	160232.0
33	240784.0
34	531452.0
35	1063225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.343635863089155	16.974512579025202	11.3523881223525	25.32946343553314
2	17.10841634185073	19.017426632844565	37.0542597290107	26.819897296294005
3	19.26086321953858	23.020864340967016	28.00957172153283	29.708700717961573
4	12.85604980636629	14.943022961486804	35.47297826536847	36.72794896677844
5	15.137234209202813	36.110653068291434	32.87478666655674	15.877326055949014
6	34.76612097367284	35.04178442928142	16.522163556194617	13.669931040851122
7	31.288430810936486	30.20000098936923	19.940613112110377	18.57095508758391
8	28.898050650037533	32.097182641646015	18.94411000489327	20.06065670342318
9	27.72512121864207	14.169039097495158	18.418514112150223	39.68732557171254
10	16.504903997731034	25.882109477544507	30.43079822704157	27.182188297682885
11	38.039160881826845	21.140420416557937	21.603867195825433	19.216551505789788
12	25.565941869165425	23.36570152963947	27.692513815400282	23.37584278579482
13	29.32747650727788	19.038464075678068	25.071781027050026	26.56227838999403
14	24.173321514708004	19.56504710271897	24.240028034878687	32.02160334769434
15	25.560787182290007	26.744603656512616	21.97800022843411	25.716608932763265
16	26.65671138435457	25.566096739122816	22.912142709604115	24.865049166918503
17	24.481670660687456	25.58726949167358	24.547600866204977	25.383458981433986
18	25.572288250152432	24.440058920119373	25.44386943413681	24.54378339559138
19	25.989514385871754	24.543155966192405	25.068673475403497	24.39865617253234
20	26.305359296915388	23.769300980271712	24.542329615023412	25.383010107789488
21	27.653267734178304	23.57710807163962	24.009694756973516	24.759929437208555
22	26.504298525848668	23.730798042050644	24.59384259310793	25.17106083899276
23	24.678484761781473	23.633400479727037	25.2626831237173	26.425431634774192
24	25.160806471097303	24.757208100333507	24.7474366411578	25.334548787411393
25	25.293469839946532	24.140082139807543	24.797217092435528	25.769230927810398
26	25.0116995787327	25.10228627292035	25.342874966859785	24.543139181487163
27	25.787851502978253	24.253994089670776	24.513725354388882	25.444429052962093
28	24.908880356950718	24.290078935315258	25.398479669631772	25.402561038102256
29	24.800341370801664	24.659422844220714	25.40227539306085	25.13796039191677
30	24.895288648794832	24.758133268692625	25.4932151991412	24.853362883371343
31	25.586655007979275	24.581361188856306	24.072633457547003	25.759350345617417
32	25.190445368832016	24.281980351496312	24.194374245041494	26.333200034630178
33	24.754347616365887	24.009041953642466	25.032548889297495	26.204061540694152
34	25.74339556675512	24.354687626263043	25.06901692522589	24.83289988175595
35	26.287885128624623	24.042020441240197	25.077152508605828	24.592941921529356
36	24.895873618485556	24.76674123800516	24.6333209368447	25.704064206664583
37	26.194499815506138	24.46285489300387	24.248924472955675	25.093720818534315
38	24.906416759562806	24.28922085974552	25.08381265542239	25.720549725269287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	136.0
1	189.5
2	243.0
3	243.0
4	641.0
5	1039.0
6	1039.0
7	1293.0
8	1547.0
9	1563.0
10	1579.0
11	1579.0
12	2016.5
13	2454.0
14	3403.5
15	4353.0
16	4353.0
17	6518.0
18	8683.0
19	8683.0
20	10767.0
21	12851.0
22	13411.0
23	13971.0
24	13971.0
25	16427.5
26	18884.0
27	18884.0
28	24422.0
29	29960.0
30	37730.5
31	45501.0
32	45501.0
33	59010.5
34	72520.0
35	72520.0
36	83332.0
37	94144.0
38	109618.0
39	125092.0
40	125092.0
41	135529.5
42	145967.0
43	167776.0
44	189585.0
45	189585.0
46	196258.5
47	202932.0
48	202932.0
49	214159.0
50	225386.0
51	230546.0
52	235706.0
53	235706.0
54	225484.0
55	215262.0
56	215262.0
57	208714.0
58	202166.0
59	182133.0
60	162100.0
61	162100.0
62	153139.5
63	144179.0
64	120910.0
65	97641.0
66	97641.0
67	83063.5
68	68486.0
69	68486.0
70	54467.0
71	40448.0
72	31422.5
73	22397.0
74	22397.0
75	17024.5
76	11652.0
77	11652.0
78	11108.5
79	10565.0
80	8330.0
81	6095.0
82	6095.0
83	5979.0
84	5863.0
85	5863.0
86	4007.0
87	2151.0
88	1831.0
89	1511.0
90	1511.0
91	1092.5
92	674.0
93	597.5
94	521.0
95	521.0
96	469.5
97	418.0
98	418.0
99	773.0
100	1128.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12313514360270263
2	0.05523973633331822
3	0.012861789355220362
4	0.003132999971143421
5	4.1223683830834485E-4
6	2.473421029850069E-4
7	8.244736766166899E-5
8	2.8856578681584144E-4
9	3.2978947064667597E-4
10	5.771315736316829E-4
11	0.002184855243034228
12	0.0022673026106958967
13	0.014758078811438747
14	0.009893684119400278
15	0.024033407673376507
16	0.011418960421141154
17	0.020529394547755576
18	0.005482749949500987
19	0.007461486773381042
20	0.005606421000993491
21	0.00614232889079434
22	0.007873723611689388
23	0.010058578854723617
24	0.014799302495269583
25	0.017025381422134646
26	0.020158381393278066
27	0.008492078869151905
28	0.005482749949500987
29	0.011336513053479486
30	0.003050552603481752
31	0.005400302581839318
32	0.006595789412933518
33	0.008574526236813573
34	0.01228465778158868
35	0.016448249848502964
36	0.01533521038507043
37	0.009687565700246106
38	0.005853763103978498
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2425790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.8359916216255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00773004554694	36.837411292591824
2	13.04471082616579	12.480133555739883
3	4.333866186954589	6.219443598252174
4	1.8316629632880161	3.5047765666194914
5	0.9506114340433349	2.2736720297159185
6	0.5732032634465197	1.6451847904629668
7	0.38789720372465103	1.2988813171197056
8	0.27871568590956536	1.066611297278846
9	0.2091379867350946	0.9003890683101248
>10	1.1774363630862716	10.263336844746943
>50	0.09414312911534692	3.1518925936112425
>100	0.08786684956976228	8.92553939479291
>500	0.01531653025315334	5.017170506471862
>1k	0.0076149980919631875	6.158497733332676
>5k	8.653406922685441E-5	0.25705941095335216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6210	0.2559990765894822	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3760	0.1550010512039377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3693	0.15223906438727178	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3561	0.14679753812160162	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3158	0.1301843935377753	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3002	0.12375349886016515	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2900	0.11954868310942002	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2875	0.11851809101364917	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2855	0.11769361733703247	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2726	0.11237576212285481	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2679	0.1104382489828056	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2559	0.10549140692310546	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2527	0.10417224904051875	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2488	0.10256452537111621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.244736766166898E-5
2	0.0	0.0	0.0	0.0	8.244736766166898E-5
3	0.0	0.0	0.0	0.0	8.244736766166898E-5
4	0.0	0.0	0.0	0.0	8.244736766166898E-5
5	0.0	0.0	0.0	0.0	8.244736766166898E-5
6	0.0	0.0	0.0	0.0	2.0611841915417245E-4
7	0.0	0.0	0.0	4.122368383083449E-5	2.473421029850069E-4
8	0.0	0.0	0.0	4.122368383083449E-5	2.473421029850069E-4
9	0.0	0.0	0.0	4.122368383083449E-5	2.473421029850069E-4
10	0.0	0.0	0.0	4.122368383083449E-5	2.473421029850069E-4
11	0.0	0.0	0.0	4.122368383083449E-5	2.8856578681584144E-4
12	0.0	0.0	0.0	4.122368383083449E-5	4.122368383083449E-4
13	0.0	0.0	0.0	4.122368383083449E-5	4.946842059700138E-4
14	0.0	0.0	0.0	4.122368383083449E-5	5.359078898008484E-4
15	0.0	0.0	0.0	1.2367105149250346E-4	7.832499927858553E-4
16	0.0	0.0	0.0	1.2367105149250346E-4	8.656973604475243E-4
17	0.0	0.0	0.0	1.2367105149250346E-4	9.069210442783589E-4
18	0.0	0.0	0.0	1.6489473532333796E-4	9.069210442783589E-4
19	0.0	0.0	0.0	1.6489473532333796E-4	9.069210442783589E-4
20	0.0	0.0	0.0	2.8856578681584144E-4	9.893684119400277E-4
21	0.0	0.0	0.0	4.122368383083449E-4	0.0010305920957708623
22	0.0	0.0	0.0	7.008026251241864E-4	0.0010718157796016968
23	0.0	0.0	0.0	0.0011954868310942001	0.0013191578825867036
24	0.0	0.0	0.0	0.001937513140049221	0.0013603815664175382
25	0.0	0.0	0.0	0.0021436315592033937	0.0013603815664175382
26	0.0	0.0	0.0	0.002597092081342573	0.001525276301740876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	255	0.0	17.589716	1
TAGGACG	250	0.0	17.277563	4
AAGACGG	790	0.0	17.009907	5
CGCTTCG	590	0.0	16.541744	32
TAAACGC	605	0.0	16.395733	28
CAAGACG	825	0.0	16.094698	4
ATCGTTT	645	0.0	15.875366	29
TCTAGCG	710	0.0	15.773723	28
CGTTTAT	655	0.0	15.632994	31
AAACGCT	625	0.0	15.615406	29
GTATCAA	5325	0.0	15.583002	1
AACGCTT	630	0.0	15.491475	30
GTAAACG	645	0.0	15.378309	27
CATCGTT	690	0.0	15.30345	28
TCGTTTA	665	0.0	15.157317	30
CTAGCGG	740	0.0	15.13456	29
TATAGGG	180	7.2759576E-12	15.116771	2
ACGCTTC	650	0.0	15.014813	31
CGGCATC	705	0.0	14.977846	25
AATCGGG	75	6.2506757E-4	14.932457	25
>>END_MODULE