FastQCFastQC Report
Thu 2 Feb 2017
SRR4062929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425790
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT66340.273477918533756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45680.18830978773925194No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37820.15590797224821604No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT36380.14997176177657587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA32100.13232802509697872No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31760.13092641984673034No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC31430.1295660382803128No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29350.12099151204349923No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28510.11752872260170914No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28250.11645690682210745No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27630.1139010384245957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27320.11262310422583982No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25880.10668689375419967No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT25760.10619220954822964No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25670.10582119639375215No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG24320.10025599907658947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG7200.018.44383828
CTAGCGG7350.017.84975229
AAGACGG8100.017.581485
GCGAATT550.001365538617.45397213
CAAGACG9000.016.1782474
CGATTAA600.002443154315.999804524
TAGCGGC8250.015.90250730
TAACGAA3350.015.76067713
ACCGTCG6300.015.7471378
TCGACGG1755.456968E-1215.54298819
GTATCAA48000.015.4667961
ATACCGT6850.015.417146
CATCGTT4900.015.34643628
ATAACGA3550.015.3234412
CGTCTTA2100.015.23790815
CTATACT1901.8189894E-1215.1583354
TAATACT3300.015.0307394
AGACGGA9700.015.0113626
ATCGTTT5050.014.890629
CGCAAGA9700.014.8454852
Produced by FastQC (version 0.11.5)