##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2425790
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219350397190194	32.0	32.0	32.0	32.0	32.0
2	31.308343261370524	32.0	32.0	32.0	32.0	32.0
3	31.38453575948454	32.0	32.0	32.0	32.0	32.0
4	31.473589634716937	32.0	32.0	32.0	32.0	32.0
5	31.41413395223824	32.0	32.0	32.0	32.0	32.0
6	34.9365002741375	36.0	36.0	36.0	36.0	36.0
7	34.955622292119266	36.0	36.0	36.0	36.0	36.0
8	34.88715964696037	36.0	36.0	36.0	32.0	36.0
9	35.00257194563421	36.0	36.0	36.0	36.0	36.0
10	34.85124598584379	36.0	36.0	36.0	32.0	36.0
11	35.02149608993359	36.0	36.0	36.0	36.0	36.0
12	34.919381314953064	36.0	36.0	36.0	32.0	36.0
13	34.973622613664006	36.0	36.0	36.0	36.0	36.0
14	34.91297185659105	36.0	36.0	36.0	32.0	36.0
15	34.881993494902694	36.0	36.0	36.0	32.0	36.0
16	34.900763462624546	36.0	36.0	36.0	32.0	36.0
17	34.86026861352384	36.0	36.0	36.0	32.0	36.0
18	34.857802612757084	36.0	36.0	36.0	32.0	36.0
19	34.85495405620437	36.0	36.0	36.0	32.0	36.0
20	34.84994001954003	36.0	36.0	36.0	32.0	36.0
21	34.83809027162285	36.0	36.0	36.0	32.0	36.0
22	34.82547376318642	36.0	36.0	36.0	32.0	36.0
23	34.75938230432148	36.0	36.0	36.0	32.0	36.0
24	34.738107173333226	36.0	36.0	36.0	32.0	36.0
25	34.71709958405303	36.0	36.0	36.0	32.0	36.0
26	34.65936705155846	36.0	36.0	36.0	32.0	36.0
27	34.64747154535224	36.0	36.0	36.0	32.0	36.0
28	34.62268333202792	36.0	36.0	36.0	32.0	36.0
29	34.58424018567147	36.0	36.0	36.0	32.0	36.0
30	34.56270534547508	36.0	36.0	36.0	32.0	36.0
31	34.56478095795597	36.0	36.0	36.0	32.0	36.0
32	34.51897897179888	36.0	36.0	36.0	32.0	36.0
33	34.48666661170176	36.0	36.0	36.0	32.0	36.0
34	34.47225852196604	36.0	36.0	36.0	32.0	36.0
35	34.43772832767882	36.0	36.0	36.0	32.0	36.0
36	34.41202948317868	36.0	36.0	36.0	32.0	36.0
37	34.41619596090346	36.0	36.0	36.0	32.0	36.0
38	34.00130225617222	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	9.0
21	43.0
22	214.0
23	611.0
24	1632.0
25	4105.0
26	8782.0
27	16523.0
28	29035.0
29	44743.0
30	67162.0
31	95705.0
32	136912.0
33	218771.0
34	539664.0
35	1261875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03317770013296	18.339857559547738	12.215742658957154	26.41122208136215
2	16.11466779894385	20.134554104023845	36.34436616524926	27.406411931783047
3	18.126630911991555	23.81665354379398	28.383949146463628	29.672766397750838
4	12.334908907725426	15.690445390393695	35.488143617192314	36.48650208468857
5	14.551671826497758	36.60481739969247	32.66502871229579	16.178482061513982
6	34.82552629441817	35.46371251155813	16.108172996655508	13.602588197368187
7	31.055549578094293	30.367028488580374	19.9151282948694	18.662293638455935
8	28.802133124901836	32.28359051290391	18.7928452260076	20.12143113618665
9	27.626749644536623	14.049512162845717	18.247958224138365	40.07577996847929
10	16.21639002575679	26.14347291249617	30.452170199293583	27.187966862453454
11	38.185534505631864	21.08727431934045	21.449525943845433	19.27766523118225
12	25.36669603175552	23.462527357146126	27.739579344071586	23.43119726702677
13	29.353108108553773	18.979018741981974	25.090444986033383	26.577428163430874
14	23.956072042509863	19.54703416206679	24.281904039508778	32.21498975591457
15	25.59244617217484	26.8754508840419	21.790138470353988	25.741964473429274
16	26.596902452396954	25.616026119326072	22.836354342296737	24.950717085980237
17	24.458135287885597	25.653539671612137	24.446180419574652	25.442144620927614
18	25.47537091009527	24.45001422217092	25.461148739173627	24.61346612856018
19	25.922895221762808	24.581023089385315	25.096566479373728	24.39951520947815
20	26.36951261238607	23.69265270283083	24.499152853297275	25.438681831485827
21	27.57729546963895	23.62332420503185	24.009260492153274	24.79011983317593
22	26.315066974085543	23.871738226201867	24.662738597627413	25.150456202085174
23	24.505716706138426	23.628969591286943	25.374383962304986	26.490929740269642
24	25.12624753173193	24.78046327175889	24.77596989022133	25.31731930628785
25	25.25356708830285	24.07893847277668	24.942369242489452	25.725125196431016
26	24.95642653026563	25.023291400567565	25.322204578471	24.69807749069581
27	25.83559410979273	24.173907953247376	24.52397962065126	25.466518316308633
28	24.878822981379262	24.2502442503267	25.518202317595506	25.352730450698534
29	24.870083560407124	24.56206019482313	25.37767077941619	25.190185465353554
30	24.842999600130266	24.64566182563206	25.590920895873097	24.920417678364572
31	25.587705448534294	24.419879709290583	24.194427382419747	25.79798745975538
32	25.24921778059931	24.250161802959035	24.290684684164745	26.209935732276907
33	24.71673145655642	23.95351617411235	25.15444453147222	26.17530783785901
34	25.641790921720347	24.24241175039884	25.273580977743332	24.84221635013748
35	26.27309041590575	23.941396411066084	25.124887150165513	24.660626022862655
36	24.972235848939935	24.668540970158176	24.666768351753447	25.692454829148442
37	26.145420667081652	24.374492433392835	24.39662955160999	25.083457347915523
38	24.987220647476203	24.131910950173715	25.126185800243057	25.754682602107025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	116.0
2	156.0
3	156.0
4	488.5
5	821.0
6	821.0
7	1129.5
8	1438.0
9	1485.5
10	1533.0
11	1533.0
12	1948.0
13	2363.0
14	3386.5
15	4410.0
16	4410.0
17	6940.0
18	9470.0
19	9470.0
20	11218.0
21	12966.0
22	13679.5
23	14393.0
24	14393.0
25	16483.5
26	18574.0
27	18574.0
28	24331.0
29	30088.0
30	37751.0
31	45414.0
32	45414.0
33	59604.0
34	73794.0
35	73794.0
36	84157.5
37	94521.0
38	110133.0
39	125745.0
40	125745.0
41	136556.0
42	147367.0
43	169584.0
44	191801.0
45	191801.0
46	197340.5
47	202880.0
48	202880.0
49	214243.0
50	225606.0
51	230413.5
52	235221.0
53	235221.0
54	225579.5
55	215938.0
56	215938.0
57	209527.5
58	203117.0
59	182570.0
60	162023.0
61	162023.0
62	152507.5
63	142992.0
64	120133.0
65	97274.0
66	97274.0
67	82801.5
68	68329.0
69	68329.0
70	54353.5
71	40378.0
72	31244.0
73	22110.0
74	22110.0
75	16643.0
76	11176.0
77	11176.0
78	10800.0
79	10424.0
80	8058.5
81	5693.0
82	5693.0
83	5418.0
84	5143.0
85	5143.0
86	3267.0
87	1391.0
88	1120.5
89	850.0
90	850.0
91	513.5
92	177.0
93	126.5
94	76.0
95	76.0
96	49.5
97	23.0
98	23.0
99	31.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008863092023629416
2	0.0
3	0.0
4	3.2978947064667597E-4
5	0.0
6	3.7101315447751043E-4
7	4.534605221391794E-4
8	0.002184855243034228
9	0.0034215657579592627
10	9.069210442783589E-4
11	0.0052766315303468155
12	2.8856578681584144E-4
13	2.473421029850069E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.1223683830834496E-5
22	4.1223683830834496E-5
23	2.0611841915417242E-4
24	0.0
25	8.244736766166899E-5
26	8.244736766166899E-5
27	4.1223683830834496E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.244736766166899E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2425790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.58882821887367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93069047205923	34.160023762523025
2	14.283586935008877	13.023439822589362
3	4.656563378576866	6.3686180386871625
4	1.9960782386471636	3.639954717324699
5	1.0240836234658521	2.3343386195973217
6	0.6447039193487961	1.7634777738736114
7	0.4314892603972253	1.3769762839376567
8	0.30546920252496185	1.1140786400053453
9	0.2311567639430899	0.9484349402729032
>10	1.2746211707924553	10.642441672641906
>50	0.10184777016340683	3.2080654699727416
>100	0.09424587643928277	9.129941454302715
>500	0.016460261937581818	5.112283087715586
>1k	0.008912186021453087	6.9028881202225385
>5k	9.094067368829679E-5	0.2750375963336716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6634	0.273477918533756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4568	0.18830978773925194	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3782	0.15590797224821604	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3638	0.14997176177657587	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3210	0.13232802509697872	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3176	0.13092641984673034	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3143	0.1295660382803128	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2935	0.12099151204349923	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2851	0.11752872260170914	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2825	0.11645690682210745	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2763	0.1139010384245957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2732	0.11262310422583982	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2588	0.10668689375419967	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2576	0.10619220954822964	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2567	0.10582119639375215	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2432	0.10025599907658947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0611841915417245E-4
2	0.0	0.0	0.0	0.0	2.0611841915417245E-4
3	0.0	0.0	0.0	0.0	2.0611841915417245E-4
4	0.0	0.0	0.0	0.0	2.473421029850069E-4
5	0.0	0.0	0.0	0.0	2.473421029850069E-4
6	0.0	0.0	0.0	0.0	3.7101315447751043E-4
7	0.0	0.0	0.0	0.0	3.7101315447751043E-4
8	0.0	0.0	0.0	0.0	3.7101315447751043E-4
9	0.0	0.0	0.0	0.0	4.122368383083449E-4
10	0.0	0.0	0.0	0.0	4.122368383083449E-4
11	0.0	0.0	0.0	0.0	4.534605221391794E-4
12	0.0	0.0	0.0	0.0	6.183552574625174E-4
13	0.0	0.0	0.0	0.0	7.832499927858553E-4
14	0.0	0.0	0.0	0.0	9.481447281091933E-4
15	0.0	0.0	0.0	8.244736766166898E-5	0.0010305920957708623
16	0.0	0.0	0.0	8.244736766166898E-5	0.0011130394634325312
17	0.0	0.0	0.0	8.244736766166898E-5	0.0011130394634325312
18	0.0	0.0	0.0	1.2367105149250346E-4	0.0011542631472633658
19	0.0	0.0	0.0	1.2367105149250346E-4	0.0011542631472633658
20	0.0	0.0	0.0	2.473421029850069E-4	0.0012779341987558693
21	0.0	0.0	0.0	3.297894706466759E-4	0.0013603815664175382
22	0.0	0.0	0.0	4.534605221391794E-4	0.0013603815664175382
23	0.0	0.0	0.0	8.244736766166898E-4	0.0014428289340792072
24	0.0	0.0	0.0	0.001525276301740876	0.0014840526179100417
25	0.0	0.0	0.0	0.0017726184047258831	0.0014840526179100417
26	0.0	0.0	0.0	0.002184855243034228	0.0015664999855717107
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	720	0.0	18.443838	28
CTAGCGG	735	0.0	17.849752	29
AAGACGG	810	0.0	17.58148	5
GCGAATT	55	0.0013655386	17.453972	13
CAAGACG	900	0.0	16.178247	4
CGATTAA	60	0.0024431543	15.9998045	24
TAGCGGC	825	0.0	15.902507	30
TAACGAA	335	0.0	15.760677	13
ACCGTCG	630	0.0	15.747137	8
TCGACGG	175	5.456968E-12	15.542988	19
GTATCAA	4800	0.0	15.466796	1
ATACCGT	685	0.0	15.41714	6
CATCGTT	490	0.0	15.346436	28
ATAACGA	355	0.0	15.32344	12
CGTCTTA	210	0.0	15.237908	15
CTATACT	190	1.8189894E-12	15.158335	4
TAATACT	330	0.0	15.030739	4
AGACGGA	970	0.0	15.011362	6
ATCGTTT	505	0.0	14.8906	29
CGCAAGA	970	0.0	14.845485	2
>>END_MODULE