FastQCFastQC Report
Thu 2 Feb 2017
SRR4062928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357328
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37320.15831483781637515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.022.739071
GTATCAA22950.021.0758041
CCTATAC2450.015.6717143
GTATAGG3550.014.4371521
CGTCTTA1002.3260083E-514.40113515
TATCAAC33000.014.25960352
GTATTAG2500.014.0942711
CTAGCGG1952.910383E-1113.94804329
GTCCTAA2900.013.8070821
GTACCGT1402.0648804E-713.7107136
TCAACGC34000.013.6917114
CAACGCA34150.013.6769655
ATCAACG34300.013.6661083
AACGCAG35400.013.1943026
GTCCTAT4150.013.1217181
TCTAGCG2201.6370905E-1113.08999828
CTAATAC2252.3646862E-1112.7985663
CGATACT1003.752817E-412.7977514
ACGCTAT1403.0709016E-612.57268710
GTATTAA3350.012.4304951
Produced by FastQC (version 0.11.5)