##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062928_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2357328
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308047077029585	32.0	32.0	32.0	32.0	32.0
2	30.93914338607101	32.0	32.0	32.0	32.0	32.0
3	30.973080538643753	32.0	32.0	32.0	32.0	32.0
4	31.045403948877713	32.0	32.0	32.0	32.0	32.0
5	30.95877620763848	32.0	32.0	32.0	32.0	32.0
6	34.6190551336089	36.0	36.0	36.0	32.0	36.0
7	34.573243943990825	36.0	36.0	36.0	32.0	36.0
8	34.557670803553854	36.0	36.0	36.0	32.0	36.0
9	34.70553779533438	36.0	36.0	36.0	32.0	36.0
10	34.436480625521774	36.0	36.0	36.0	32.0	36.0
11	34.67272013058853	36.0	36.0	36.0	32.0	36.0
12	34.51618527417483	36.0	36.0	36.0	32.0	36.0
13	34.57852577155152	36.0	36.0	36.0	32.0	36.0
14	34.49511099007011	36.0	36.0	36.0	32.0	36.0
15	34.44150114027407	36.0	36.0	36.0	32.0	36.0
16	34.459780734798045	36.0	36.0	36.0	32.0	36.0
17	34.40889176219856	36.0	36.0	36.0	32.0	36.0
18	34.420709379432985	36.0	36.0	36.0	32.0	36.0
19	34.411380597014926	36.0	36.0	36.0	32.0	36.0
20	34.39987943977249	36.0	36.0	36.0	32.0	36.0
21	34.39313536342842	36.0	36.0	36.0	32.0	36.0
22	34.364872007628975	36.0	36.0	36.0	32.0	36.0
23	34.31017787936172	36.0	36.0	36.0	32.0	36.0
24	34.29628418276965	36.0	36.0	36.0	32.0	36.0
25	34.27417058635879	36.0	36.0	36.0	32.0	36.0
26	34.235208676942705	36.0	36.0	36.0	32.0	36.0
27	34.24387823841231	36.0	36.0	36.0	32.0	36.0
28	34.22321246767527	36.0	36.0	36.0	32.0	36.0
29	34.1934821967923	36.0	36.0	36.0	32.0	36.0
30	34.173810347987214	36.0	36.0	36.0	32.0	36.0
31	34.1844104850916	36.0	36.0	36.0	32.0	36.0
32	34.149603279645426	36.0	36.0	36.0	32.0	36.0
33	34.127217765198566	36.0	36.0	36.0	32.0	36.0
34	34.125999860859416	36.0	36.0	36.0	32.0	36.0
35	34.09990209253867	36.0	36.0	36.0	32.0	36.0
36	34.06043791954281	36.0	36.0	36.0	32.0	36.0
37	34.05664464172996	36.0	36.0	36.0	32.0	36.0
38	33.524526073588405	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	2.0
9	11.0
10	16.0
11	34.0
12	8.0
13	13.0
14	145.0
15	411.0
16	602.0
17	698.0
18	872.0
19	1024.0
20	1432.0
21	2118.0
22	3392.0
23	5512.0
24	8648.0
25	13629.0
26	20281.0
27	30022.0
28	42231.0
29	57555.0
30	77198.0
31	105551.0
32	144118.0
33	218544.0
34	503201.0
35	1120055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.126641119152694	17.0232573547493	11.496593641208767	26.35350788488924
2	16.5403471808691	19.37465169649538	37.801832973057174	26.283168149578344
3	19.116877783382666	22.99029005939156	28.802169134632887	29.09066302259289
4	13.165703755332991	15.48903579196364	35.7205247955148	35.62473565718858
5	14.83075095712301	36.03567603109457	34.133435143647986	15.000137868134434
6	34.4440706682962	35.215581403142465	16.801770482874005	13.538577445687332
7	30.287172595413114	30.427076758092213	20.778058887011056	18.50769175948362
8	27.966504335431765	33.69122562910424	19.238542073201774	19.10372796226223
9	27.360612016257424	14.617428328659246	18.45945591673267	39.562503738350664
10	15.7728044212976	27.092028776783096	32.35135537562407	24.783811426295234
11	36.781607244972584	21.44581287893314	22.468518747910725	19.304061128183548
12	24.383458436100867	23.64153836103072	29.09129320372534	22.883709999143072
13	29.784921327892516	19.87392437329179	24.805791095283066	25.53536320353263
14	23.4917411996494	19.198412301102785	25.46834656839994	31.84149993084787
15	25.200571971689946	27.022777032875645	22.213929292757854	25.562721702676555
16	25.60670560194546	25.939957761190357	23.778506741833308	24.67482989503087
17	24.2454676293181	25.923658279107816	25.15923972260975	24.671634368964334
18	24.589914958208787	25.726605446984845	25.59772339484998	24.08575619995639
19	25.378203096728104	25.170705259312903	25.42758512141657	24.02350652254243
20	25.731824757444244	24.833976047751772	25.137473008115595	24.29672618668839
21	26.09031168731996	24.651637344782092	24.760920934849842	24.497130033048105
22	25.724513483977386	24.60117395111422	25.226797580825806	24.447514984082595
23	25.071559875899247	24.644300643540394	25.222125163918463	25.062014316641896
24	24.79077128031939	24.999809078528124	25.288694478975305	24.920725162177185
25	25.04837875484265	24.57551646786934	25.390095476178047	24.986009301109966
26	24.914951049651275	25.189509172727654	25.436403581347868	24.459136196273207
27	25.0862172466736	25.232793990311087	24.977907427134813	24.7030813358805
28	25.101562208337434	24.78024551412368	25.297517028851473	24.82067524868741
29	24.76794292577525	25.162712536024586	25.245272628614075	24.824071909586092
30	24.92779728259855	25.224032050879586	25.131678751359633	24.71649191516223
31	25.091051479668742	24.904387991937835	25.103608841570633	24.90095168682279
32	25.00179240892548	25.09644008496655	25.07913114197024	24.822636364137736
33	24.860412578883174	24.968892188577186	25.261112584186247	24.909582648353396
34	25.266498829467054	24.842281397987144	25.272396041485827	24.61882373105998
35	25.13951263388567	24.991100805912716	25.356657212377836	24.51272934782378
36	24.894981648040343	25.120567983711283	25.119931573134817	24.864518795113554
37	25.1669955313714	24.745672540695153	25.10950961330008	24.977822314633368
38	24.67847227878689	25.083234910808827	25.299255382693925	24.939037427710353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	81.5
2	138.0
3	138.0
4	353.5
5	569.0
6	569.0
7	677.5
8	786.0
9	757.5
10	729.0
11	729.0
12	1129.5
13	1530.0
14	2396.0
15	3262.0
16	3262.0
17	5183.5
18	7105.0
19	7105.0
20	9530.5
21	11956.0
22	13972.5
23	15989.0
24	15989.0
25	20427.0
26	24865.0
27	24865.0
28	31580.5
29	38296.0
30	46696.5
31	55097.0
32	55097.0
33	68175.0
34	81253.0
35	81253.0
36	92378.0
37	103503.0
38	117690.0
39	131877.0
40	131877.0
41	146699.5
42	161522.0
43	175355.0
44	189188.0
45	189188.0
46	197152.5
47	205117.0
48	205117.0
49	211656.0
50	218195.0
51	219272.0
52	220349.0
53	220349.0
54	215479.5
55	210610.0
56	210610.0
57	198758.5
58	186907.0
59	171024.0
60	155141.0
61	155141.0
62	137596.5
63	120052.0
64	100123.0
65	80194.0
66	80194.0
67	66601.5
68	53009.0
69	53009.0
70	42631.5
71	32254.0
72	25467.0
73	18680.0
74	18680.0
75	15109.5
76	11539.0
77	11539.0
78	9459.5
79	7380.0
80	5777.5
81	4175.0
82	4175.0
83	3331.0
84	2487.0
85	2487.0
86	1907.5
87	1328.0
88	965.5
89	603.0
90	603.0
91	448.0
92	293.0
93	244.0
94	195.0
95	195.0
96	174.0
97	153.0
98	153.0
99	565.0
100	977.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11712413376500852
2	0.0536624517250039
3	0.010987015807728071
4	0.002842201000454752
5	1.2726273136364562E-4
6	2.1210455227274272E-4
7	0.0
8	3.3936728363638833E-4
9	2.1210455227274272E-4
10	4.666300150000339E-4
11	0.0022058873436365243
12	0.0027997800900002034
13	0.013998900450001017
14	0.008738707553637
15	0.02409507713818357
16	0.010774911255455329
17	0.021380138869092465
18	0.005684402000909504
19	0.008272077538636964
20	0.0058540856427276985
21	0.006702503851818669
22	0.006787345672727767
23	0.008950812105909742
24	0.014338267733637406
25	0.017180468734092156
26	0.019089409704546843
27	0.008526603001364257
28	0.006447978389091379
29	0.010902173986818975
30	0.0032239891945456895
31	0.006151032015909538
32	0.006999450225000509
33	0.008611444822273355
34	0.011962696748182688
35	0.015822999599546604
36	0.015229106853182926
37	0.009714388494091616
38	0.006108611105454989
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2357328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.352220702657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.74293134914287	23.51017019875156
2	15.650382776041443	12.317546341676893
3	8.03285773388422	9.48332371250571
4	4.723958381644574	7.435930112985748
5	3.05891525775954	6.018755416703918
6	2.0794142710704415	4.909774159645118
7	1.4675578021476419	4.042616096681416
8	1.0547642479407016	3.3205852379387615
9	0.8070526460504381	2.8583382461436146
>10	3.264226849722243	20.409534800974075
>50	0.07789126900867843	2.10946036408985
>100	0.037344333659216	2.673078232886991
>500	0.002378712096553427	0.6027904170603325
>1k	3.2436983134804214E-4	0.30809666195611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3732	0.15831483781637515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.242091045454854E-5	0.0
16	0.0	0.0	0.0	8.484182090909708E-5	0.0
17	0.0	0.0	0.0	1.2726273136364562E-4	0.0
18	0.0	0.0	0.0	1.6968364181819417E-4	0.0
19	0.0	0.0	0.0	2.121045522727427E-4	0.0
20	0.0	0.0	0.0	2.5452546272729124E-4	0.0
21	0.0	0.0	0.0	2.5452546272729124E-4	0.0
22	0.0	0.0	0.0	2.969463731818398E-4	0.0
23	0.0	0.0	0.0	3.3936728363638833E-4	0.0
24	0.0	0.0	0.0	5.938927463636796E-4	0.0
25	0.0	0.0	0.0	6.363136568182281E-4	0.0
26	0.0	0.0	0.0	8.908391195455194E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	810	0.0	22.73907	1
GTATCAA	2295	0.0	21.075804	1
CCTATAC	245	0.0	15.671714	3
GTATAGG	355	0.0	14.437152	1
CGTCTTA	100	2.3260083E-5	14.401135	15
TATCAAC	3300	0.0	14.2596035	2
GTATTAG	250	0.0	14.094271	1
CTAGCGG	195	2.910383E-11	13.948043	29
GTCCTAA	290	0.0	13.807082	1
GTACCGT	140	2.0648804E-7	13.710713	6
TCAACGC	3400	0.0	13.691711	4
CAACGCA	3415	0.0	13.676965	5
ATCAACG	3430	0.0	13.666108	3
AACGCAG	3540	0.0	13.194302	6
GTCCTAT	415	0.0	13.121718	1
TCTAGCG	220	1.6370905E-11	13.089998	28
CTAATAC	225	2.3646862E-11	12.798566	3
CGATACT	100	3.752817E-4	12.797751	4
ACGCTAT	140	3.0709016E-6	12.572687	10
GTATTAA	335	0.0	12.430495	1
>>END_MODULE