FastQCFastQC Report
Thu 2 Feb 2017
SRR4062928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357328
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36020.15280011945728386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24000.1018101850909165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.021.9443131
GGTATCA7800.020.309041
CGCGATC951.4342022E-515.15729223
TCTAGCG2350.014.978127528
CTAGCGG2350.014.978127529
CGATCGT1401.2811142E-814.85655225
GTATAGG3250.014.770211
CGCGTAA1308.3147825E-814.7702110
ATGCGTA650.004164404214.76864322
TATCAAC31750.014.4630312
GTCTAGG2250.014.22316551
ATCAACG32500.014.17883
TCAACGC32350.014.1462244
CAACGCA33250.013.7641945
GTATTAT2850.013.4745771
AACGCAG34150.013.354596
TCGCGTA1453.1523086E-713.2425389
GTACTAT2550.013.1773421
TTCCGCG850.001609659413.17594625
GATCGTT1352.045872E-613.03651826
Produced by FastQC (version 0.11.5)