##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2357328
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261213543469555	32.0	32.0	32.0	32.0	32.0
2	31.334849880882082	32.0	32.0	32.0	32.0	32.0
3	31.39758871060794	32.0	32.0	32.0	32.0	32.0
4	31.48456472752201	32.0	32.0	32.0	32.0	32.0
5	31.42466215986914	32.0	32.0	32.0	32.0	32.0
6	34.95179414998677	36.0	36.0	36.0	36.0	36.0
7	34.97702271385229	36.0	36.0	36.0	36.0	36.0
8	34.91483408333503	36.0	36.0	36.0	36.0	36.0
9	35.00914552408489	36.0	36.0	36.0	36.0	36.0
10	34.88179201197288	36.0	36.0	36.0	32.0	36.0
11	35.04170060339503	36.0	36.0	36.0	36.0	36.0
12	34.95904091411972	36.0	36.0	36.0	36.0	36.0
13	35.00331434573381	36.0	36.0	36.0	36.0	36.0
14	34.94856210081923	36.0	36.0	36.0	32.0	36.0
15	34.916257729089885	36.0	36.0	36.0	32.0	36.0
16	34.9471214867002	36.0	36.0	36.0	36.0	36.0
17	34.91777682189326	36.0	36.0	36.0	32.0	36.0
18	34.91154434172928	36.0	36.0	36.0	32.0	36.0
19	34.91054660191539	36.0	36.0	36.0	32.0	36.0
20	34.902283432767945	36.0	36.0	36.0	32.0	36.0
21	34.89165063156251	36.0	36.0	36.0	32.0	36.0
22	34.87198344905757	36.0	36.0	36.0	32.0	36.0
23	34.818248881784804	36.0	36.0	36.0	32.0	36.0
24	34.808288452010075	36.0	36.0	36.0	32.0	36.0
25	34.79945175215329	36.0	36.0	36.0	32.0	36.0
26	34.731942266837706	36.0	36.0	36.0	32.0	36.0
27	34.71457684293404	36.0	36.0	36.0	32.0	36.0
28	34.6972529915226	36.0	36.0	36.0	32.0	36.0
29	34.66917416668363	36.0	36.0	36.0	32.0	36.0
30	34.65014923676298	36.0	36.0	36.0	32.0	36.0
31	34.6382060536336	36.0	36.0	36.0	32.0	36.0
32	34.618924901413806	36.0	36.0	36.0	32.0	36.0
33	34.598914109534185	36.0	36.0	36.0	32.0	36.0
34	34.59378202778739	36.0	36.0	36.0	32.0	36.0
35	34.57698292303829	36.0	36.0	36.0	32.0	36.0
36	34.5529065111007	36.0	36.0	36.0	32.0	36.0
37	34.547469847216846	36.0	36.0	36.0	32.0	36.0
38	34.161604579422125	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	7.0
21	37.0
22	163.0
23	524.0
24	1361.0
25	3558.0
26	7628.0
27	14800.0
28	26160.0
29	40929.0
30	62292.0
31	88867.0
32	126958.0
33	202599.0
34	507352.0
35	1274090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52171408134789	18.23673132067179	12.059466015813426	27.182088582166898
2	15.665921613759991	20.29718405634857	37.01170860046146	27.025185729429985
3	18.1498713797995	23.74438347145582	29.034525530600746	29.071219618143935
4	12.686026455399041	16.358068421592943	35.57352695384516	35.38237816916286
5	14.344297587903586	36.575791139710354	33.871075162673655	15.208836109712397
6	34.497676178582154	35.60961497019502	16.55343092421746	13.339277927005366
7	30.167215737911878	30.52092338992599	20.755699071061386	18.556161801100746
8	27.826157782921218	33.86311418661635	19.15653062194137	19.154197408521064
9	27.23891281744101	14.471954384114701	18.33056244800613	39.95857035043816
10	15.52611986993622	27.330489690557652	32.39648666591722	24.746903773588908
11	36.96924959857119	21.360509586565446	22.422233110824045	19.24800770403932
12	24.274844198138478	23.705765994533625	29.04118700847275	22.97820279885514
13	29.818094816714012	19.852612726193694	24.81942471282092	25.509867744271368
14	23.42609938031534	19.134672816001846	25.50260294706549	31.936624856617325
15	25.153054644920008	27.13597768320743	22.099894456774788	25.611073215097775
16	25.5979760126618	25.995460112237296	23.70612986658194	24.70043400851897
17	24.20923180821676	25.99909728302553	25.10321007513592	24.688460833621797
18	24.589068640426788	25.746523182179143	25.531788533458222	24.132619643935847
19	25.39795056097412	25.26691236858002	25.319896085737753	24.01524098470811
20	25.722300842309597	24.879821560682263	25.083993402700006	24.313884194308134
21	26.063567815214277	24.72374407432153	24.76383188571792	24.448856224746272
22	25.608867327754137	24.738899296152255	25.136637752574103	24.51559562351951
23	25.002290733051545	24.721378987360243	25.13290493797204	25.143425341616172
24	24.793622270638622	25.07033386953364	25.212062131362288	24.92398172846545
25	25.007784243672702	24.662095951090347	25.230112423992267	25.100007381244684
26	24.886460336839285	25.24199028899694	25.369337970225587	24.50221140393819
27	25.136298385290463	25.160054095145014	24.91184934807545	24.791798171489077
28	25.04738415697773	24.83735822931726	25.287189563777297	24.828068049927715
29	24.803082133669985	25.067746193995916	25.16030862060774	24.968863051726363
30	24.934120326064086	25.190130520657288	25.121620750273188	24.75412840300544
31	25.056928861830002	24.87837924972681	25.037245559379095	25.02744632906409
32	24.993424758879545	25.172016791893192	24.981122694847727	24.853435754379536
33	24.878421670637263	24.91774585462863	25.24162950594911	24.962202968784997
34	25.201711429211375	24.818735449627713	25.298431105047747	24.681122016113157
35	25.049886990694546	25.005472297448637	25.324816911350478	24.619823800506335
36	24.891869099251355	25.058710540069097	25.078775630714095	24.970644729965453
37	25.10248891965819	24.86158056918681	25.08051488804273	24.95541562311227
38	24.73292843971159	25.021263490385508	25.303914136647144	24.94189393325576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	44.5
2	77.0
3	77.0
4	258.5
5	440.0
6	440.0
7	558.0
8	676.0
9	619.5
10	563.0
11	563.0
12	969.0
13	1375.0
14	2252.5
15	3130.0
16	3130.0
17	5045.0
18	6960.0
19	6960.0
20	9327.0
21	11694.0
22	13958.5
23	16223.0
24	16223.0
25	20497.0
26	24771.0
27	24771.0
28	31651.5
29	38532.0
30	46952.0
31	55372.0
32	55372.0
33	68861.5
34	82351.0
35	82351.0
36	93404.5
37	104458.0
38	118592.5
39	132727.0
40	132727.0
41	147454.0
42	162181.0
43	176358.5
44	190536.0
45	190536.0
46	197600.0
47	204664.0
48	204664.0
49	212465.5
50	220267.0
51	220225.5
52	220184.0
53	220184.0
54	215152.5
55	210121.0
56	210121.0
57	198798.0
58	187475.0
59	171259.5
60	155044.0
61	155044.0
62	137345.5
63	119647.0
64	99596.0
65	79545.0
66	79545.0
67	66005.5
68	52466.0
69	52466.0
70	42115.5
71	31765.0
72	25044.0
73	18323.0
74	18323.0
75	14731.5
76	11140.0
77	11140.0
78	9090.0
79	7040.0
80	5455.0
81	3870.0
82	3870.0
83	2985.0
84	2100.0
85	2100.0
86	1590.0
87	1080.0
88	711.5
89	343.0
90	343.0
91	226.0
92	109.0
93	76.0
94	43.0
95	43.0
96	30.5
97	18.0
98	18.0
99	12.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009205337568637032
2	4.242091045454854E-5
3	0.0
4	8.484182090909708E-5
5	4.242091045454854E-5
6	1.6968364181819417E-4
7	5.938927463636796E-4
8	0.0027149382690911067
9	0.0033512519259093344
10	9.756809404546163E-4
11	0.00521777198590947
12	2.969463731818398E-4
13	4.242091045454854E-5
14	0.0
15	0.0
16	4.242091045454854E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2726273136364562E-4
22	0.0
23	1.6968364181819417E-4
24	0.0
25	8.484182090909708E-5
26	8.484182090909708E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.242091045454854E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2357328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.891486148311756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.851304684453126	20.604376331316423
2	17.09471374313067	12.612987905281194
3	8.748495699371956	9.682350237358365
4	5.2074971875413265	7.684492414462133
5	3.3093180592339384	6.104283067129341
6	2.270402772779511	5.025511946585037
7	1.6090816900046205	4.155299041481527
8	1.1674456966476616	3.4455045397426707
9	0.8817733217641445	2.92769354572315
>10	3.726771523021045	21.755771208109163
>50	0.08777342748254469	2.2071706225674808
>100	0.04252817865720525	2.8812097855138754
>500	0.0025467340028206558	0.6020239329323224
>1k	3.472819094754378E-4	0.3113254217972286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3602	0.15280011945728386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2400	0.1018101850909165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.242091045454854E-5	0.0
16	0.0	0.0	0.0	4.242091045454854E-5	0.0
17	0.0	0.0	0.0	8.484182090909708E-5	0.0
18	0.0	0.0	0.0	1.2726273136364562E-4	0.0
19	0.0	0.0	0.0	1.2726273136364562E-4	0.0
20	0.0	0.0	0.0	1.6968364181819417E-4	0.0
21	0.0	0.0	0.0	3.3936728363638833E-4	0.0
22	0.0	0.0	0.0	3.817881940909369E-4	0.0
23	0.0	0.0	0.0	4.242091045454854E-4	0.0
24	0.0	0.0	0.0	5.090509254545825E-4	0.0
25	0.0	0.0	0.0	5.51471835909131E-4	0.0
26	0.0	0.0	0.0	7.211554777273252E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2100	0.0	21.944313	1
GGTATCA	780	0.0	20.30904	1
CGCGATC	95	1.4342022E-5	15.157292	23
TCTAGCG	235	0.0	14.9781275	28
CTAGCGG	235	0.0	14.9781275	29
CGATCGT	140	1.2811142E-8	14.856552	25
GTATAGG	325	0.0	14.77021	1
CGCGTAA	130	8.3147825E-8	14.77021	10
ATGCGTA	65	0.0041644042	14.768643	22
TATCAAC	3175	0.0	14.463031	2
GTCTAGG	225	0.0	14.2231655	1
ATCAACG	3250	0.0	14.1788	3
TCAACGC	3235	0.0	14.146224	4
CAACGCA	3325	0.0	13.764194	5
GTATTAT	285	0.0	13.474577	1
AACGCAG	3415	0.0	13.35459	6
TCGCGTA	145	3.1523086E-7	13.242538	9
GTACTAT	255	0.0	13.177342	1
TTCCGCG	85	0.0016096594	13.175946	25
GATCGTT	135	2.045872E-6	13.036518	26
>>END_MODULE