FastQCFastQC Report
Thu 2 Feb 2017
SRR4062927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884270
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41820.14499336053836848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.020.7523771
GTATCAA29400.019.9920391
TATACCG859.4560586E-515.055835
GTATAGG2700.014.8290931
TATCAAC40550.014.2487212
TCAACGC40350.014.1535644
ACGAACG2500.014.08012915
ATCAACG40800.014.0383713
CTAGCGG3200.013.999886529
AGCGTAT2201.8189894E-1213.8171118
TAGCGTA2201.8189894E-1213.8171117
CCGATAA2550.013.8038099
TCTAGCG3250.013.78426528
CGTATAT2450.013.71417410
TAGCGGC3150.013.71417430
GTCTAGA4800.013.6798381
CCCTTAT2350.013.6301451
AACGCAG42300.013.5764976
CAACGCA42200.013.5707615
GTATATA5100.013.5031981
Produced by FastQC (version 0.11.5)