##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062927_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2884270
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28362185232312	32.0	32.0	32.0	32.0	32.0
2	30.89890890936008	32.0	32.0	32.0	32.0	32.0
3	30.935215149760598	32.0	32.0	32.0	32.0	32.0
4	31.01151348521463	32.0	32.0	32.0	32.0	32.0
5	30.910970193497835	32.0	32.0	32.0	32.0	32.0
6	34.5772902675547	36.0	36.0	36.0	32.0	36.0
7	34.530225672353836	36.0	36.0	36.0	32.0	36.0
8	34.50730722158467	36.0	36.0	36.0	32.0	36.0
9	34.65961577799582	36.0	36.0	36.0	32.0	36.0
10	34.3752023908996	36.0	36.0	36.0	32.0	36.0
11	34.63462851952141	36.0	36.0	36.0	32.0	36.0
12	34.47085605716524	36.0	36.0	36.0	32.0	36.0
13	34.53285476047665	36.0	36.0	36.0	32.0	36.0
14	34.444483699514954	36.0	36.0	36.0	32.0	36.0
15	34.3900889999896	36.0	36.0	36.0	32.0	36.0
16	34.406881463940614	36.0	36.0	36.0	32.0	36.0
17	34.354832938663854	36.0	36.0	36.0	32.0	36.0
18	34.36987452630995	36.0	36.0	36.0	32.0	36.0
19	34.35556587975467	36.0	36.0	36.0	32.0	36.0
20	34.34172043532679	36.0	36.0	36.0	32.0	36.0
21	34.33321949748117	36.0	36.0	36.0	32.0	36.0
22	34.311626165372864	36.0	36.0	36.0	32.0	36.0
23	34.25058437663603	36.0	36.0	36.0	32.0	36.0
24	34.239266088126286	36.0	36.0	36.0	32.0	36.0
25	34.222757578174026	36.0	36.0	36.0	32.0	36.0
26	34.17352883051864	36.0	36.0	36.0	32.0	36.0
27	34.17817367999528	36.0	36.0	36.0	32.0	36.0
28	34.15953672853096	36.0	36.0	36.0	32.0	36.0
29	34.126651804442716	36.0	36.0	36.0	32.0	36.0
30	34.101853501925966	36.0	36.0	36.0	32.0	36.0
31	34.11887409985889	36.0	36.0	36.0	32.0	36.0
32	34.0776518841856	36.0	36.0	36.0	32.0	36.0
33	34.05420643698405	36.0	36.0	36.0	32.0	36.0
34	34.05894143058729	36.0	36.0	36.0	32.0	36.0
35	34.028816650313594	36.0	36.0	36.0	32.0	36.0
36	33.9932499384593	36.0	36.0	36.0	32.0	36.0
37	33.98948919483959	36.0	36.0	36.0	32.0	36.0
38	33.43346843395383	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	9.0
8	5.0
9	10.0
10	31.0
11	34.0
12	21.0
13	16.0
14	264.0
15	581.0
16	803.0
17	904.0
18	1118.0
19	1394.0
20	1993.0
21	2945.0
22	4551.0
23	7239.0
24	11414.0
25	17647.0
26	26595.0
27	37975.0
28	53659.0
29	72975.0
30	98357.0
31	132672.0
32	181072.0
33	272806.0
34	614335.0
35	1342843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.274277744183316	16.881030948018687	11.395684342289517	26.44900696550848
2	16.52394839732905	19.35443586419627	37.90289653622153	26.218719202253148
3	19.227475103331575	23.20151044411773	28.48747538073178	29.083539071818915
4	12.969530712856987	15.558548122639928	36.02462118388466	35.44729998061843
5	14.792707589713682	36.03847643363512	34.249108696549094	14.919707280102099
6	34.35186042326931	35.33956508127033	16.88344648182222	13.425128013638146
7	30.18338442080125	30.258204071811612	20.916183615328528	18.64222789205861
8	27.770608593267315	33.59917871424817	19.375485826857375	19.254726865627138
9	27.447024503355806	14.517113240216375	18.168832330533537	39.867029925894286
10	15.385404817122492	27.019266340366123	32.66755724965875	24.927771592852636
11	37.08462961865028	21.244309086426995	22.485483858105688	19.185577436817034
12	24.582307622963892	23.39313080320923	29.40059219604879	22.623969377778085
13	29.74216251154714	19.834477181749836	24.775853794534495	25.647506512168533
14	23.398294306356338	19.274386717281764	25.544246369957158	31.783072606404744
15	25.036031499645578	26.965294626000663	22.371746587931423	25.62692728642233
16	25.5260408557849	25.893904503859115	23.704406700673278	24.875647939682704
17	24.201556764044756	25.762170064993477	25.285313110982727	24.75096005997904
18	24.67325779677249	25.51161931126689	25.83342059403386	23.981702297926756
19	25.554244113594297	25.10105692821612	25.30733164633508	24.037367311854503
20	25.61555414088711	24.907179719756954	25.069727898232635	24.4075382411233
21	26.301912856302774	24.628614344203182	24.690124768382393	24.379348031111654
22	25.635406701550856	24.610517475786395	25.076637844618915	24.677437978043834
23	25.019409015838935	24.597941240006392	25.08556784070841	25.297081903446255
24	24.821350055411997	24.9727101113372	25.30230400173102	24.90363583151979
25	25.056722564501438	24.686374412965236	25.272169480746232	24.984733541787097
26	24.86065746304861	25.28396471087182	25.32821309689807	24.5271647291815
27	25.15359865907391	25.080643847020973	24.96129476760737	24.80446272629775
28	24.965474294602476	24.88090668680478	25.191577357517104	24.962041661075634
29	24.805396504297416	25.1439609894932	25.16355223633216	24.887090269877227
30	24.814321827833922	25.23375114461522	25.25084452753439	24.70108250001647
31	25.136741998577712	25.02447053324785	24.800100968038	25.038686500136436
32	25.07714829393049	25.112688519224314	24.772161150823703	25.03800203602149
33	24.809292649098474	24.807073509015257	25.15891123439667	25.224722607489596
34	25.07717919617126	24.85404176952314	25.298721545643655	24.770057488661948
35	25.188034324369873	24.904968544779045	25.376606137357122	24.53039099349396
36	24.841417654905815	25.130997530023407	25.11320870521975	24.914376109851023
37	25.25534299318577	24.946427902706137	25.064217167759377	24.734011936348722
38	24.839480985630782	24.998144990329678	25.20996282352582	24.952411200513716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	108.5
2	182.0
3	182.0
4	399.0
5	616.0
6	616.0
7	775.5
8	935.0
9	939.5
10	944.0
11	944.0
12	1355.5
13	1767.0
14	2701.5
15	3636.0
16	3636.0
17	5874.0
18	8112.0
19	8112.0
20	11127.0
21	14142.0
22	17015.0
23	19888.0
24	19888.0
25	25044.5
26	30201.0
27	30201.0
28	39195.5
29	48190.0
30	59063.0
31	69936.0
32	69936.0
33	84684.5
34	99433.0
35	99433.0
36	112676.5
37	125920.0
38	143929.5
39	161939.0
40	161939.0
41	175765.5
42	189592.0
43	209212.5
44	228833.0
45	228833.0
46	238554.0
47	248275.0
48	248275.0
49	259110.5
50	269946.0
51	271144.5
52	272343.0
53	272343.0
54	264834.0
55	257325.0
56	257325.0
57	244850.5
58	232376.0
59	210161.5
60	187947.0
61	187947.0
62	169460.0
63	150973.0
64	125808.0
65	100643.0
66	100643.0
67	82973.0
68	65303.0
69	65303.0
70	52291.5
71	39280.0
72	30518.5
73	21757.0
74	21757.0
75	17038.5
76	12320.0
77	12320.0
78	10585.0
79	8850.0
80	6803.0
81	4756.0
82	4756.0
83	4122.5
84	3489.0
85	3489.0
86	2485.5
87	1482.0
88	1135.5
89	789.0
90	789.0
91	602.5
92	416.0
93	353.5
94	291.0
95	291.0
96	274.0
97	257.0
98	257.0
99	704.0
100	1151.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12148654598910644
2	0.05377443859278084
3	0.012134786271742937
4	0.0030163611589761013
5	3.813789971119209E-4
6	2.4269572543485874E-4
7	3.467081791926553E-5
8	4.1604981503118635E-4
9	4.1604981503118635E-4
10	5.894039046275141E-4
11	0.0023229448005907907
12	0.0031203736127338984
13	0.015185818248638305
14	0.00977717065323288
15	0.023645497820939092
16	0.011129332552084236
17	0.020663807479882258
18	0.006656797040498982
19	0.008737046115654915
20	0.007003505219691637
21	0.0071421884913687
22	0.008702375297735649
23	0.010435916193698925
24	0.014492401890252993
25	0.01723139650587497
26	0.01903427903767678
27	0.010158549650344802
28	0.006483442950902655
29	0.01140669909543836
30	0.003917802424877006
31	0.006552784586741187
32	0.007419555034722824
33	0.009361120838201694
34	0.013764314713948417
35	0.01691935914460158
36	0.015151147430719037
37	0.010609270283295254
38	0.006518113768821921
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2884270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.38110568037699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12176428128105	36.2067443963189
2	16.053718957600957	16.818230985623348
3	6.4176263307434995	10.084870891435358
4	3.107619473363165	6.511221761945338
5	1.720217548849314	4.50534486097575
6	0.9658560645046607	3.0355565152110993
7	0.6257604715759517	2.294461777455573
8	0.4433714969490874	1.8579431389885677
9	0.3005761811958856	1.4170061441003248
>10	1.1602753097989966	9.97747518043097
>50	0.04397905850569414	1.5936429255910416
>100	0.035110895369595746	3.754123688496464
>500	0.003059690194588628	1.1570097130215344
>1k	0.0010642400676145406	0.786368020405793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4182	0.14499336053836848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.467081791926553E-5	0.0
4	0.0	0.0	0.0	1.7335408959632767E-4	0.0
5	6.934163583853106E-5	0.0	0.0	1.7335408959632767E-4	0.0
6	6.934163583853106E-5	0.0	0.0	1.7335408959632767E-4	0.0
7	6.934163583853106E-5	0.0	0.0	2.080249075155932E-4	0.0
8	6.934163583853106E-5	0.0	0.0	2.4269572543485874E-4	0.0
9	6.934163583853106E-5	0.0	0.0	4.160498150311864E-4	0.0
10	2.080249075155932E-4	0.0	0.0	4.507206329504519E-4	0.0
11	2.4269572543485874E-4	0.0	0.0	5.20062268788983E-4	0.0
12	2.4269572543485874E-4	0.0	0.0	5.89403904627514E-4	6.934163583853106E-5
13	2.4269572543485874E-4	0.0	0.0	5.89403904627514E-4	2.080249075155932E-4
14	2.4269572543485874E-4	0.0	0.0	5.89403904627514E-4	2.080249075155932E-4
15	2.4269572543485874E-4	0.0	0.0	6.240747225467796E-4	2.7736654335412425E-4
16	2.4269572543485874E-4	0.0	0.0	6.934163583853107E-4	2.7736654335412425E-4
17	2.4269572543485874E-4	0.0	0.0	7.974288121431073E-4	2.7736654335412425E-4
18	2.4269572543485874E-4	0.0	0.0	9.014412659009038E-4	3.120373612733898E-4
19	2.4269572543485874E-4	0.0	0.0	9.014412659009038E-4	3.120373612733898E-4
20	2.4269572543485874E-4	0.0	0.0	0.001109466173416497	3.120373612733898E-4
21	2.4269572543485874E-4	0.0	0.0	0.0013521618988513557	3.120373612733898E-4
22	2.4269572543485874E-4	0.0	0.0	0.001698870078044011	3.120373612733898E-4
23	2.4269572543485874E-4	0.0	0.0	0.0021495907109944632	3.120373612733898E-4
24	2.4269572543485874E-4	0.0	0.0	0.0024962988901871186	3.120373612733898E-4
25	2.4269572543485874E-4	0.0	0.0	0.0029123487052183047	3.120373612733898E-4
26	2.4269572543485874E-4	0.0	0.0	0.0033283985202494913	3.120373612733898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1065	0.0	20.752377	1
GTATCAA	2940	0.0	19.992039	1
TATACCG	85	9.4560586E-5	15.05583	5
GTATAGG	270	0.0	14.829093	1
TATCAAC	4055	0.0	14.248721	2
TCAACGC	4035	0.0	14.153564	4
ACGAACG	250	0.0	14.080129	15
ATCAACG	4080	0.0	14.038371	3
CTAGCGG	320	0.0	13.9998865	29
AGCGTAT	220	1.8189894E-12	13.817111	8
TAGCGTA	220	1.8189894E-12	13.817111	7
CCGATAA	255	0.0	13.803809	9
TCTAGCG	325	0.0	13.784265	28
CGTATAT	245	0.0	13.714174	10
TAGCGGC	315	0.0	13.714174	30
GTCTAGA	480	0.0	13.679838	1
CCCTTAT	235	0.0	13.630145	1
AACGCAG	4230	0.0	13.576497	6
CAACGCA	4220	0.0	13.570761	5
GTATATA	510	0.0	13.503198	1
>>END_MODULE