FastQCFastQC Report
Thu 2 Feb 2017
SRR4062927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884270
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40290.13968872539672084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.021.0503751
GGTATCA9950.018.3322941
CGCGTAA1401.8189894E-1218.28793310
TCGCGTA1703.6379788E-1216.0024979
ACCGTCG3400.015.0614358
CGATAAC1757.4578566E-1114.63034810
TATCAAC40900.014.356472
ATACCGT4150.014.2675336
ATCAACG41850.013.9163683
TCAACGC42150.013.7795994
CAACGCA42600.013.6737325
GCGTAAC1902.7830538E-1013.47508511
GTATTAG3800.013.4741531
AACGCAG43400.013.4216826
CTAGCGG2750.013.38112329
TAAACGC2900.013.2406928
TCTAGCG2800.013.14217428
AATCCCG1954.2200554E-1013.12752219
GTATTAC2850.012.9127281
TACACCG1504.755002E-712.8022195
Produced by FastQC (version 0.11.5)