##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2884270
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22554823230835	32.0	32.0	32.0	32.0	32.0
2	31.2666595013643	32.0	32.0	32.0	32.0	32.0
3	31.356797387207163	32.0	32.0	32.0	32.0	32.0
4	31.462244519410458	32.0	32.0	32.0	32.0	32.0
5	31.382137594607993	32.0	32.0	32.0	32.0	32.0
6	34.90218183457166	36.0	36.0	36.0	36.0	36.0
7	34.94271618121743	36.0	36.0	36.0	36.0	36.0
8	34.865660288391865	36.0	36.0	36.0	32.0	36.0
9	34.98756843152687	36.0	36.0	36.0	36.0	36.0
10	34.83480534069279	36.0	36.0	36.0	32.0	36.0
11	35.01231923502307	36.0	36.0	36.0	36.0	36.0
12	34.92347873118675	36.0	36.0	36.0	32.0	36.0
13	34.96838853505393	36.0	36.0	36.0	36.0	36.0
14	34.91447367964857	36.0	36.0	36.0	32.0	36.0
15	34.892654293807446	36.0	36.0	36.0	32.0	36.0
16	34.904590416292514	36.0	36.0	36.0	32.0	36.0
17	34.87866461877702	36.0	36.0	36.0	32.0	36.0
18	34.873999660225984	36.0	36.0	36.0	32.0	36.0
19	34.873037198320546	36.0	36.0	36.0	32.0	36.0
20	34.85949061634313	36.0	36.0	36.0	32.0	36.0
21	34.857514379721735	36.0	36.0	36.0	32.0	36.0
22	34.837256220811504	36.0	36.0	36.0	32.0	36.0
23	34.7818508669438	36.0	36.0	36.0	32.0	36.0
24	34.76855218131451	36.0	36.0	36.0	32.0	36.0
25	34.75610397084878	36.0	36.0	36.0	32.0	36.0
26	34.688154715057884	36.0	36.0	36.0	32.0	36.0
27	34.67146765039334	36.0	36.0	36.0	32.0	36.0
28	34.64976025129409	36.0	36.0	36.0	32.0	36.0
29	34.6201652411182	36.0	36.0	36.0	32.0	36.0
30	34.60316613909239	36.0	36.0	36.0	32.0	36.0
31	34.59646808377857	36.0	36.0	36.0	32.0	36.0
32	34.57297999147098	36.0	36.0	36.0	32.0	36.0
33	34.55187205081355	36.0	36.0	36.0	32.0	36.0
34	34.537707634860816	36.0	36.0	36.0	32.0	36.0
35	34.526176814237225	36.0	36.0	36.0	32.0	36.0
36	34.49691048341521	36.0	36.0	36.0	32.0	36.0
37	34.49652841100174	36.0	36.0	36.0	32.0	36.0
38	34.08905823657286	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	52.0
22	170.0
23	644.0
24	1787.0
25	4451.0
26	9661.0
27	18979.0
28	32454.0
29	51566.0
30	78027.0
31	112609.0
32	163525.0
33	261523.0
34	645956.0
35	1502852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.589133551663224	18.076188924121407	12.078508606924721	27.25616891729065
2	15.595211266628992	20.307911533941	37.06338172223821	27.033495477191806
3	18.15388295825287	24.042790723475957	28.697764078952385	29.10556223931879
4	12.475981724267768	16.443825144872413	35.95318317129304	35.12700995956678
5	14.338181248318463	36.68332718502883	33.74413715249367	15.234354414159037
6	34.40749417442976	35.78781021313214	16.535847756119665	13.268847856318434
7	30.082794200245473	30.390117395796494	20.792126923370294	18.734961480587742
8	27.697678595327236	33.822180277248265	19.136315694947857	19.343825432476645
9	27.294425781156544	14.377045877940594	17.923494349987674	40.405033990915186
10	15.11604241939057	27.367142966604458	32.62577719591977	24.891037418085205
11	37.178626583072884	21.1950600983459	22.371993262383562	19.254320056197656
12	24.50409238843834	23.494993684682054	29.337156848366842	22.663757078512766
13	29.784156448869993	19.852787451915827	24.72588337063342	25.637172728580765
14	23.303782239526814	19.25038224576756	25.444635904405622	32.001199610300006
15	24.97838274502734	27.161812174311006	22.215881314856098	25.64392376580556
16	25.546759177718574	25.98908977945184	23.598985947214338	24.86516509561525
17	24.214792651173433	25.799457054991382	25.20613534793899	24.77961494589619
18	24.72518176176294	25.569034799099942	25.681818969791316	24.023964469345795
19	25.481005592402926	25.167304031869413	25.301376084763216	24.05031429096444
20	25.660773783314323	24.885915673636656	24.958273670634163	24.495036872414857
21	26.254347288689097	24.626725177159273	24.632653889079002	24.486273645072636
22	25.56032433890482	24.63804495214902	25.061168054136456	24.740462654809697
23	24.961289978108816	24.55973200807415	25.075287785523248	25.403690228293783
24	24.837861920000552	25.0414836336404	25.171360517565972	24.94929392879307
25	24.992025706349065	24.782613820907073	25.24446410666415	24.980896366079712
26	24.92256272817732	25.262718122783234	25.177219885794326	24.637499263245118
27	25.16248478817864	25.10139480700489	24.88387009537942	24.85225030943705
28	24.959591161715096	24.932755948645585	25.142063676424193	24.96558921321513
29	24.77281946558401	25.059304434050905	25.111380002565642	25.056496097799442
30	24.86643067396603	25.26826545365032	25.08173645324467	24.783567419138986
31	25.12077579422176	24.97647585004178	24.808599749676695	25.09414860605976
32	25.068353517527832	25.041448962822482	24.851868930439938	25.038328589209748
33	24.891636358593335	24.786930488477154	25.075218339475846	25.246214813453665
34	25.09574346368405	24.87163129665392	25.244273247650185	24.788351992011844
35	25.186338310907093	24.871839321561435	25.313233504491606	24.62858886303987
36	24.871804650743513	25.088150554559736	25.058229638695405	24.981815156001343
37	25.264174297135845	24.868510923041185	24.998838527599705	24.868476252223264
38	24.812248788167818	24.97326012240883	25.1576742668593	25.056816822564056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	49.5
2	82.0
3	82.0
4	254.5
5	427.0
6	427.0
7	576.0
8	725.0
9	739.5
10	754.0
11	754.0
12	1176.0
13	1598.0
14	2636.5
15	3675.0
16	3675.0
17	5962.0
18	8249.0
19	8249.0
20	11064.5
21	13880.0
22	16835.0
23	19790.0
24	19790.0
25	25043.0
26	30296.0
27	30296.0
28	39298.5
29	48301.0
30	59489.0
31	70677.0
32	70677.0
33	85088.5
34	99500.0
35	99500.0
36	112965.0
37	126430.0
38	144505.5
39	162581.0
40	162581.0
41	176990.0
42	191399.0
43	209763.0
44	228127.0
45	228127.0
46	237582.5
47	247038.0
48	247038.0
49	259460.0
50	271882.0
51	272463.5
52	273045.0
53	273045.0
54	265207.0
55	257369.0
56	257369.0
57	245109.5
58	232850.0
59	210441.5
60	188033.0
61	188033.0
62	169584.5
63	151136.0
64	126082.5
65	101029.0
66	101029.0
67	82929.5
68	64830.0
69	64830.0
70	52013.0
71	39196.0
72	30421.0
73	21646.0
74	21646.0
75	16839.0
76	12032.0
77	12032.0
78	10218.5
79	8405.0
80	6477.5
81	4550.0
82	4550.0
83	3817.5
84	3085.0
85	3085.0
86	2053.0
87	1021.0
88	721.0
89	421.0
90	421.0
91	262.5
92	104.0
93	79.0
94	54.0
95	54.0
96	38.0
97	22.0
98	22.0
99	18.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00884105856941271
2	0.0
3	0.0
4	2.7736654335412425E-4
5	2.0802490751559318E-4
6	1.0401245375779659E-4
7	3.4670817919265533E-4
8	0.0023576156185100565
9	0.004056485696554067
10	7.974288121431073E-4
11	0.005686014138759547
12	4.507206329504519E-4
13	1.7335408959632767E-4
14	0.0
15	0.0
16	6.934163583853106E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	3.467081791926553E-5
22	1.0401245375779659E-4
23	1.3868327167706213E-4
24	0.0
25	6.934163583853106E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.467081791926553E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2884270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37271747376636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.81868276122817	33.6583862870057
2	17.18891523593851	17.317047417208972
3	6.8414410270571295	10.338659279081483
4	3.3591975522843356	6.768476369591453
5	1.8349586675391105	4.621592726799319
6	1.1073571578311945	3.3468353552390195
7	0.7223052754690369	2.5469135699708945
8	0.44663447192264455	1.799855365456337
9	0.3262311806173465	1.4789835983133757
>10	1.263229084650674	10.44846712657986
>50	0.04876211520505896	1.6932486103549145
>100	0.03750100373076603	3.850616980249285
>500	0.0037443651074504525	1.3562732643364
>1k	0.0010401014186322535	0.7746440498128221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4029	0.13968872539672084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.934163583853106E-5
2	0.0	0.0	0.0	0.0	6.934163583853106E-5
3	0.0	0.0	0.0	3.467081791926553E-5	6.934163583853106E-5
4	0.0	0.0	0.0	6.934163583853106E-5	6.934163583853106E-5
5	3.467081791926553E-5	0.0	0.0	6.934163583853106E-5	6.934163583853106E-5
6	3.467081791926553E-5	0.0	0.0	1.040124537577966E-4	1.040124537577966E-4
7	3.467081791926553E-5	0.0	0.0	1.040124537577966E-4	1.040124537577966E-4
8	3.467081791926553E-5	0.0	0.0	1.040124537577966E-4	1.040124537577966E-4
9	3.467081791926553E-5	0.0	0.0	1.040124537577966E-4	1.040124537577966E-4
10	1.3868327167706213E-4	0.0	0.0	1.3868327167706213E-4	1.040124537577966E-4
11	1.7335408959632767E-4	0.0	0.0	2.080249075155932E-4	1.040124537577966E-4
12	1.7335408959632767E-4	0.0	0.0	2.080249075155932E-4	1.3868327167706213E-4
13	1.7335408959632767E-4	0.0	0.0	2.080249075155932E-4	1.3868327167706213E-4
14	1.7335408959632767E-4	0.0	0.0	2.080249075155932E-4	1.3868327167706213E-4
15	1.7335408959632767E-4	0.0	0.0	2.4269572543485874E-4	2.080249075155932E-4
16	1.7335408959632767E-4	0.0	0.0	3.120373612733898E-4	2.7736654335412425E-4
17	1.7335408959632767E-4	0.0	0.0	4.160498150311864E-4	2.7736654335412425E-4
18	1.7335408959632767E-4	0.0	0.0	5.20062268788983E-4	2.7736654335412425E-4
19	1.7335408959632767E-4	0.0	0.0	5.20062268788983E-4	2.7736654335412425E-4
20	1.7335408959632767E-4	0.0	0.0	7.280871763045762E-4	3.4670817919265533E-4
21	1.7335408959632767E-4	0.0	0.0	9.70782901739435E-4	3.4670817919265533E-4
22	1.7335408959632767E-4	0.0	0.0	0.0013868327167706213	3.4670817919265533E-4
23	1.7335408959632767E-4	0.0	0.0	0.0017682117138825423	3.4670817919265533E-4
24	1.7335408959632767E-4	0.0	0.0	0.0020455782572366666	3.4670817919265533E-4
25	1.7335408959632767E-4	0.0	0.0	0.002392286436429322	3.4670817919265533E-4
26	1.7335408959632767E-4	0.0	0.0	0.002669652979783446	4.160498150311864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2820	0.0	21.050375	1
GGTATCA	995	0.0	18.332294	1
CGCGTAA	140	1.8189894E-12	18.287933	10
TCGCGTA	170	3.6379788E-12	16.002497	9
ACCGTCG	340	0.0	15.061435	8
CGATAAC	175	7.4578566E-11	14.630348	10
TATCAAC	4090	0.0	14.35647	2
ATACCGT	415	0.0	14.267533	6
ATCAACG	4185	0.0	13.916368	3
TCAACGC	4215	0.0	13.779599	4
CAACGCA	4260	0.0	13.673732	5
GCGTAAC	190	2.7830538E-10	13.475085	11
GTATTAG	380	0.0	13.474153	1
AACGCAG	4340	0.0	13.421682	6
CTAGCGG	275	0.0	13.381123	29
TAAACGC	290	0.0	13.24069	28
TCTAGCG	280	0.0	13.142174	28
AATCCCG	195	4.2200554E-10	13.127522	19
GTATTAC	285	0.0	12.912728	1
TACACCG	150	4.755002E-7	12.802219	5
>>END_MODULE