FastQCFastQC Report
Thu 2 Feb 2017
SRR4062926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448182
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT68791.534867531493902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT47781.0660847602090222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27570.6151518802629289No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA27480.6131437674873154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23730.5294724018367538No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA23030.5138537469153157No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22510.5022513175451044No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14130.31527370577131614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13920.3105881092948847No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT12850.28671387962925776No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA12760.2847057668536443No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11540.2574846825619949No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11280.2516834678768893No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA11090.2474441186839275No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10740.2396347912232084No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA9490.21174433600635456No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9440.2106287177976804No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.2077281104551276No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.19791067021879505No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8750.19523318651797708No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8420.18787010634072765No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA8370.1867544881320535No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA8360.18653136449031865No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG7920.1767139242539861No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG7820.1744826878366378No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC7780.17359019326969846No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT7640.17046646228541085No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7420.16555774216724456No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA7310.16310338210816142No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC7280.16243401118295692No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC7160.15975652748213895No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC7130.15908715655693445No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC6970.1555171782891772No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6960.15529405464744234No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6780.15127782909621537No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6600.14726160354498843No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG6480.14458411984417044No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.14101414157641318No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA6290.14034477065120865No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG6260.13967539972600415No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6230.13900602880079968No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA6130.13677479238345136No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT6080.13565917417477721No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6060.13521292689130754No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT6040.1347666796078379No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT5940.13253544319048957No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG5870.13097357769834575No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA5820.1298579594896716No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC5810.12963483584793678No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT5780.12896546492273228No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA5670.12651110486364914No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG5630.12561861029670982No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5530.12338737387936152No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5210.11624741734384693No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5020.11200806815088514No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC5020.11200806815088514No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4980.11111557358394582No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC4980.11111557358394582No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT4960.11066932630047614No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4900.10933058445006716No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA4900.10933058445006716No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4900.10933058445006716No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA4710.10509123525710537No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA4670.10419874069016605No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4660.10397561704843122No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA4620.10308312248149189No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4590.10241375155628739No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4570.10196750427281774No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT4540.10129813334761324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT200.003749938232.0020124
GAACTTA356.4192864E-527.43029411
GCAAGTT401.5907583E-424.00150725
TGAACTT401.5907583E-424.00150710
TGTTATG350.002063830822.8585782
TTAAGTC350.002069194822.8483774
TTGGTTC350.002069194822.8483773
AGGCTTA350.002069194822.8483776
TAGGACC2850.021.3251534
TTAGGAC4200.020.1827343
GAGCATT400.004477656420.00125511
CAGCCAT400.0044805519.99902317
TTGGACA400.004489240719.992334
TATACCA400.004489240719.992335
TAGAAAT1850.019.8842644
TAGGGGG659.407655E-619.69354429
TTAGAAA1800.019.5480563
GGACCTG4550.019.3332426
CTGAATT507.1835297E-419.20120410
ATTGAAC507.188928E-419.1990628
Produced by FastQC (version 0.11.5)