FastQCFastQC Report
Thu 2 Feb 2017
SRR4062926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448182
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT90712.0239545541766515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT65711.466145449839574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT40170.8962876688488158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.7269368247720792No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA28450.634786760735594No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC27140.6055575636683311No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA19230.4290667630560799No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT13920.3105881092948847No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.30902624380274085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.29474633073171164No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA13110.29251509431436334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.2726570901999634No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11630.2594927953376084No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11510.2568153116367904No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11120.248113489609132No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA10480.2338335765381028No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA9740.21732242704972532No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9370.2090668523055366No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA9360.20884372866380174No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8770.19567943380144676No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA8610.19210945553368944No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA8580.19144008460848494No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG8470.18898572454940182No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT8310.1854157462816445No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA8310.1854157462816445No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA8190.18273826258082654No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7870.17559830604531196No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG7330.16354962939163106No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC7290.16265713482469174No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7280.16243401118295692No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC7270.1622108875412221No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC7080.1579715383482603No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA6760.15083158181274572No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC6750.1506084581710109No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6720.1499390872458064No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT6720.1499390872458064No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG6720.1499390872458064No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT6650.14837722175366258No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG6640.14815409811192773No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA6610.14748472718672326No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA6600.14726160354498843No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC6590.14703847990325358No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG6520.14547661441110976No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC6450.14391474891896594No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6440.14369162527723112No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA6430.1434685016354963No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT6370.14212975978508732No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6340.14146038885988282No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC6280.14012164700947383No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6080.13565917417477721No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA6020.13432043232436822No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG6020.13432043232436822No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG5940.13253544319048957No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT5710.12740359943058846No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA5590.12472611572977049No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG5530.12338737387936152No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5380.12004051925333904No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5270.1175861591942559No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5240.1169167882690514No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5230.11669366462731658No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC5210.11624741734384693No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5060.11290056271782446No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT5060.11290056271782446No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA5010.11178494450915029No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT4910.10955370809180198No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT4900.10933058445006716No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4840.10799184259965817No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA4810.10732247167445368No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG4770.10642997710751435No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA4760.10620685346577953No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4750.10598372982404469No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4630.10330624612322672No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA4580.10219062791455256No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT4500.10040563878067393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG200.003751604631.99910715
TGACTAT200.003751604631.99910728
TAGGACC2650.024.1529644
ATATAAG350.002063581722.8590553
CTCTAGG350.002063581722.8590551
ACGAATA350.00206492122.85650418
TCCTCGC1900.020.20996329
ACCTACT400.004477116320.0016758
CACTAAT400.004477116320.0016751
CGAATAT400.004480008519.99944319
CCCGGAA400.004480008519.99944329
AGGACCT4600.019.8277455
CATATAG659.405972E-619.6939543
CGTATGT751.504779E-619.19946523
GAACACT507.1879185E-419.19946524
ATGTCCA1950.018.8733759
TGTAGGA8550.018.7129272
AATTCAT601.1364584E-418.66614518
TAGGACA2150.018.6062074
ACAACAA953.7753125E-818.52579921
Produced by FastQC (version 0.11.5)