##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448182
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.754160140300147	32.0	32.0	32.0	32.0	32.0
2	31.31385017693705	32.0	32.0	32.0	32.0	32.0
3	31.420429646884525	32.0	32.0	32.0	32.0	32.0
4	31.532854956245455	32.0	32.0	32.0	32.0	32.0
5	31.447702049613774	32.0	32.0	32.0	32.0	32.0
6	34.90198401542231	36.0	36.0	36.0	36.0	36.0
7	34.9619819626848	36.0	36.0	36.0	36.0	36.0
8	34.93286878991124	36.0	36.0	36.0	36.0	36.0
9	34.99929046681928	36.0	36.0	36.0	36.0	36.0
10	34.89122053094502	36.0	36.0	36.0	32.0	36.0
11	35.04684480858223	36.0	36.0	36.0	36.0	36.0
12	34.97733510047257	36.0	36.0	36.0	36.0	36.0
13	34.9992926980557	36.0	36.0	36.0	36.0	36.0
14	34.95158216974354	36.0	36.0	36.0	36.0	36.0
15	34.9353075313154	36.0	36.0	36.0	36.0	36.0
16	34.928002463285004	36.0	36.0	36.0	36.0	36.0
17	34.909407338982824	36.0	36.0	36.0	32.0	36.0
18	34.88982377694776	36.0	36.0	36.0	36.0	36.0
19	34.85623028144816	36.0	36.0	36.0	32.0	36.0
20	34.80704490586414	36.0	36.0	36.0	32.0	36.0
21	34.758490970186216	36.0	36.0	36.0	32.0	36.0
22	34.76523599787586	36.0	36.0	36.0	32.0	36.0
23	34.73660700340486	36.0	36.0	36.0	32.0	36.0
24	34.722389564953524	36.0	36.0	36.0	32.0	36.0
25	34.716894029657595	36.0	36.0	36.0	32.0	36.0
26	34.6445238764609	36.0	36.0	36.0	32.0	36.0
27	34.609991030429605	36.0	36.0	36.0	32.0	36.0
28	34.5701589979071	36.0	36.0	36.0	32.0	36.0
29	34.497280122807254	36.0	36.0	36.0	32.0	36.0
30	34.44023187008849	36.0	36.0	36.0	32.0	36.0
31	34.39125176825486	36.0	36.0	36.0	32.0	36.0
32	34.33533028992686	36.0	36.0	36.0	32.0	36.0
33	34.25087129782097	36.0	36.0	36.0	32.0	36.0
34	34.15428776702322	36.0	36.0	36.0	32.0	36.0
35	34.030025748468255	36.0	36.0	36.0	32.0	36.0
36	33.94487953554583	36.0	36.0	36.0	32.0	36.0
37	33.875889705521416	36.0	36.0	36.0	32.0	36.0
38	33.517684779843904	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	4.0
21	22.0
22	37.0
23	122.0
24	316.0
25	762.0
26	1630.0
27	3312.0
28	5897.0
29	9427.0
30	14073.0
31	20292.0
32	27937.0
33	42372.0
34	95998.0
35	225977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.639219886642564	22.39590306600616	14.162761636988442	21.80211541036283
2	11.667358349956045	24.203337037185786	43.389962113605634	20.739342499252537
3	17.07743729110049	31.331244896046694	32.03118376016886	19.560134052683956
4	9.031152522859017	20.066401595780288	41.17479059846223	29.727655282898464
5	8.640259181000532	43.11963246991729	35.626454490484875	12.613653858597306
6	24.837286721213083	42.32307929162548	20.068900734301543	12.770733252859895
7	22.86710056874343	36.82887047866822	23.4117465934522	16.89228235913615
8	25.116529181941715	36.73196168426783	21.289641676949202	16.86186745684125
9	26.70150525925893	15.599269906864036	22.975174155774027	34.724050678103005
10	15.18169120304879	29.373820223214885	34.96847234804029	20.47601622569604
11	29.625373477361528	24.830582412702803	27.864590887487083	17.679453222448583
12	23.533966111981293	29.27315242468461	32.57091984952542	14.621961613808676
13	27.651551493704552	24.28193073780459	29.54855292839277	18.517964840098085
14	20.646523064290847	25.75850882007756	30.878750150608457	22.716217965023137
15	21.3054071783338	32.49371906948517	29.69864920947294	16.502224542708095
16	18.420864737985905	29.710474762484886	32.5983640574588	19.270296442070407
17	16.32082502197768	29.746174545162457	33.04818131919622	20.884819113663646
18	17.163116769526667	26.73266663989183	38.628280475342606	17.4759361152389
19	20.33303434765341	28.26976540780308	34.06919510377481	17.328005140768706
20	20.36092480287026	28.011388230674143	35.72611126729766	15.901575699157933
21	21.25502866029573	25.987268536595703	32.25705685872449	20.500645944384075
22	19.768129394151025	29.986545614383473	32.5439945022212	17.701330489244302
23	18.35816699465842	29.614085349255436	34.15286646942537	17.874881186660776
24	19.463075268529302	30.33365909385027	33.717329120758976	16.485936516861454
25	19.608372510213506	28.748206639728146	34.60811591745299	17.035304932605353
26	15.813888107956142	29.341874506338943	37.222601532413165	17.621635853291743
27	17.813745308825432	31.0340442052559	34.57702451236328	16.57518597355539
28	16.475003458416445	29.664288168645776	35.220289971484796	18.640418401452983
29	17.721818368430682	28.753051215800724	34.936030451914625	18.58909996385397
30	18.244373937373656	28.436438768179002	35.34367734536416	17.975509949083186
31	18.177436844853208	28.162889183412098	34.72607110504215	18.93360286669255
32	15.581393273268448	31.30826316094801	35.035766719770095	18.07457684601345
33	16.618918207335412	30.96956147279453	35.12390055825535	17.2876197616147
34	16.55086549660629	30.60899366775105	36.739092600773795	16.10104823486887
35	18.11518534880919	31.415808756264198	33.661771333967	16.80723456095961
36	16.796301502514606	30.396579960819487	34.94093917203279	17.866179364633116
37	18.169404393750753	30.86023088834447	32.09388150349635	18.87648321440843
38	17.410337764568858	32.838891343248946	32.576721064210524	17.174049827971672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	801.0
1	613.5
2	426.0
3	426.0
4	1435.5
5	2445.0
6	2445.0
7	2854.0
8	3263.0
9	3222.0
10	3181.0
11	3181.0
12	3921.5
13	4662.0
14	5671.0
15	6680.0
16	6680.0
17	9317.5
18	11955.0
19	11955.0
20	12924.0
21	13893.0
22	13485.5
23	13078.0
24	13078.0
25	13909.5
26	14741.0
27	14741.0
28	19768.0
29	24795.0
30	29682.5
31	34570.0
32	34570.0
33	38174.0
34	41778.0
35	41778.0
36	41768.5
37	41759.0
38	42946.5
39	44134.0
40	44134.0
41	40816.0
42	37498.0
43	36094.5
44	34691.0
45	34691.0
46	36633.5
47	38576.0
48	38576.0
49	33861.5
50	29147.0
51	21799.0
52	14451.0
53	14451.0
54	12342.0
55	10233.0
56	10233.0
57	8176.0
58	6119.0
59	5317.0
60	4515.0
61	4515.0
62	4016.5
63	3518.0
64	3046.5
65	2575.0
66	2575.0
67	2146.5
68	1718.0
69	1718.0
70	1490.0
71	1262.0
72	1030.0
73	798.0
74	798.0
75	621.0
76	444.0
77	444.0
78	353.0
79	262.0
80	182.5
81	103.0
82	103.0
83	81.5
84	60.0
85	60.0
86	39.5
87	19.0
88	15.5
89	12.0
90	12.0
91	8.0
92	4.0
93	5.5
94	7.0
95	7.0
96	7.0
97	7.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0093711929528629
2	0.0
3	0.0
4	0.0
5	2.2312364173483095E-4
6	2.2312364173483095E-4
7	2.2312364173483095E-4
8	0.0024543600590831403
9	0.0044624728346966186
10	8.924945669393238E-4
11	0.006470585610310098
12	0.0
13	2.2312364173483095E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.2312364173483095E-4
22	2.2312364173483095E-4
23	0.0
24	0.0
25	2.2312364173483095E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	448182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.428259780337637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45805316364739	22.65628984401653
2	13.240087535204555	8.05745646071229
3	4.4039177175442115	4.020106570820006
4	2.0695542956556423	2.5189174295089423
5	1.1490514180899964	1.748181752530388
6	0.7624035279717057	1.3919167563941384
7	0.4894435459383618	1.0425040754535464
8	0.38585564945787354	0.9392732751532058
9	0.29706261143187934	0.813518848050725
>10	2.019189854872177	12.507674493683588
>50	0.3483880921015776	7.50874474130553
>100	0.33005187672781033	20.26234043120435
>500	0.03520553351763311	7.270393147198997
>1k	0.010268280609309656	5.771768658036666
>5k	0.0014668972299013794	3.4909135159310773
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9071	2.0239545541766515	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6571	1.466145449839574	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4017	0.8962876688488158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.7269368247720792	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2845	0.634786760735594	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2714	0.6055575636683311	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1923	0.4290667630560799	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1392	0.3105881092948847	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1385	0.30902624380274085	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.29474633073171164	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1311	0.29251509431436334	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1222	0.2726570901999634	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1163	0.2594927953376084	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1151	0.2568153116367904	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1112	0.248113489609132	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1048	0.2338335765381028	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	974	0.21732242704972532	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	937	0.2090668523055366	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	936	0.20884372866380174	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	877	0.19567943380144676	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	861	0.19210945553368944	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	858	0.19144008460848494	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	847	0.18898572454940182	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	831	0.1854157462816445	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	831	0.1854157462816445	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	819	0.18273826258082654	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	787	0.17559830604531196	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	733	0.16354962939163106	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	729	0.16265713482469174	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	728	0.16243401118295692	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	727	0.1622108875412221	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	708	0.1579715383482603	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	676	0.15083158181274572	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	675	0.1506084581710109	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	672	0.1499390872458064	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	672	0.1499390872458064	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	672	0.1499390872458064	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	665	0.14837722175366258	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	664	0.14815409811192773	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	661	0.14748472718672326	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	660	0.14726160354498843	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	659	0.14703847990325358	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	652	0.14547661441110976	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	645	0.14391474891896594	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	644	0.14369162527723112	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	643	0.1434685016354963	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	637	0.14212975978508732	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	634	0.14146038885988282	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	628	0.14012164700947383	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	608	0.13565917417477721	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	602	0.13432043232436822	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	602	0.13432043232436822	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	594	0.13253544319048957	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	571	0.12740359943058846	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	559	0.12472611572977049	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	553	0.12338737387936152	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	538	0.12004051925333904	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	527	0.1175861591942559	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.1169167882690514	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	523	0.11669366462731658	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	521	0.11624741734384693	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	506	0.11290056271782446	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	506	0.11290056271782446	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	501	0.11178494450915029	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	491	0.10955370809180198	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT	490	0.10933058445006716	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	484	0.10799184259965817	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	481	0.10732247167445368	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	477	0.10642997710751435	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA	476	0.10620685346577953	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	475	0.10598372982404469	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	463	0.10330624612322672	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	458	0.10219062791455256	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	450	0.10040563878067393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
6	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
7	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
8	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
9	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
10	0.0	2.2312364173483095E-4	0.0	2.2312364173483095E-4	0.0
11	0.0	2.2312364173483095E-4	0.0	6.693709252044928E-4	0.0
12	0.0	2.2312364173483095E-4	0.0	8.924945669393238E-4	0.0
13	0.0	2.2312364173483095E-4	0.0	8.924945669393238E-4	0.0
14	0.0	2.2312364173483095E-4	0.0	8.924945669393238E-4	0.0
15	0.0	2.2312364173483095E-4	0.0	8.924945669393238E-4	0.0
16	0.0	2.2312364173483095E-4	0.0	0.0015618654921438165	0.0
17	0.0	2.2312364173483095E-4	0.0	0.0017849891338786476	0.0
18	0.0	2.2312364173483095E-4	0.0	0.0020081127756134787	0.0
19	0.0	8.924945669393238E-4	0.0	0.0024543600590831403	0.0
20	0.0	8.924945669393238E-4	0.0	0.002900607342552802	0.0
21	0.0	8.924945669393238E-4	0.0	0.0044624728346966186	0.0
22	0.0	8.924945669393238E-4	0.0	0.008032451102453915	0.0
23	0.0	8.924945669393238E-4	0.0	0.015395531279703336	0.0
24	0.0	8.924945669393238E-4	0.0	0.024543600590831403	0.0
25	0.0	8.924945669393238E-4	0.0	0.030344815275937007	0.0
26	0.0	8.924945669393238E-4	0.0	0.0368154008862471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCG	20	0.0037516046	31.999107	15
TGACTAT	20	0.0037516046	31.999107	28
TAGGACC	265	0.0	24.152964	4
ATATAAG	35	0.0020635817	22.859055	3
CTCTAGG	35	0.0020635817	22.859055	1
ACGAATA	35	0.002064921	22.856504	18
TCCTCGC	190	0.0	20.209963	29
ACCTACT	40	0.0044771163	20.001675	8
CACTAAT	40	0.0044771163	20.001675	1
CGAATAT	40	0.0044800085	19.999443	19
CCCGGAA	40	0.0044800085	19.999443	29
AGGACCT	460	0.0	19.827745	5
CATATAG	65	9.405972E-6	19.693954	3
CGTATGT	75	1.504779E-6	19.199465	23
GAACACT	50	7.1879185E-4	19.199465	24
ATGTCCA	195	0.0	18.873375	9
TGTAGGA	855	0.0	18.712927	2
AATTCAT	60	1.1364584E-4	18.666145	18
TAGGACA	215	0.0	18.606207	4
ACAACAA	95	3.7753125E-8	18.525799	21
>>END_MODULE