FastQCFastQC Report
Thu 2 Feb 2017
SRR4062925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588352
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT63640.17735160876079045No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT53340.1486476243133338No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC52340.1458608297067846No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA46720.1301990440179782No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG45310.1262696636227438No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC44440.12384515231504603No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC42530.11852237461653706No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG42380.11810435542555467No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG41700.11620933509310125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40000.11147178426196762No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG38440.10712438467575086No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG37360.10411464650067774No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG36990.10308353249625454No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC36080.10054754940429479No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT35980.10026886994363986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG10800.019.8507228
CTAGCGG11450.018.44487629
TAGCGGC11950.017.80676530
ATCGTTT9200.017.39079729
CATCGTT9550.016.92050228
GTATCAA56150.016.802561
AGCGGCG12800.016.49928931
CGCCGGT13750.016.1729477
GTCCTAT13100.015.773511
CGGTCCA14250.015.71905910
TCGTTTA10300.015.53331130
TAGGACG7050.015.4306694
AGGCCCG12800.015.37476510
CAAGACG13900.015.1923184
GCGGCGC13800.015.18775132
AAGACGG13650.015.1183315
GCATCGT10800.014.96190127
CGGCATC10550.014.86212525
CCGGTCC16050.014.853159
CGTTTAT10700.014.80310131
Produced by FastQC (version 0.11.5)