FastQCFastQC Report
Thu 2 Feb 2017
SRR4062925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588352
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT68550.191034770278947No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC56630.15781617856888064No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT53120.148034529499893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA48870.1361906524220589No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC47380.13203832845830063No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG46130.12855483520011415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT46120.12852696725404866No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC43430.12103048976243133No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG43010.11986003602768068No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG41580.11587491974031532No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC40990.11423071092245131No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA38320.10678996932296497No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT37550.1046441374759221No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG37290.1039195708782193No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG36960.10299992865805807No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA36010.10035247378183634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG10900.018.78865629
TCTAGCG11000.018.76330228
TAGCGGC11650.017.57908430
GTATCAA56250.016.7833251
CGCCGGT12800.016.375627
ATCGTTT8200.015.99971429
CGGTCCA14150.015.94384510
CAAGACG13700.015.7665824
AAGACGG13850.015.7113495
AGCGGCG13500.015.64416531
CGCAAGA13750.015.5926652
ACCGTCG8950.015.1961068
GCGCAAG14550.014.8463371
CCGGTCC15450.014.8094169
TAGGACG5300.014.7926014
ATACCGT9750.014.7695856
TACCGTC9450.014.7307187
CATCGTT8800.014.7270128
TCGTTTA9200.014.60843630
GCCGGTC14550.014.5160158
Produced by FastQC (version 0.11.5)