##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3588352
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26576517576871	32.0	32.0	32.0	32.0	32.0
2	31.33608993766498	32.0	32.0	32.0	32.0	32.0
3	31.40374299957195	32.0	32.0	32.0	32.0	32.0
4	31.49831900549333	32.0	32.0	32.0	32.0	32.0
5	31.430373887511593	32.0	32.0	32.0	32.0	32.0
6	34.97004864628665	36.0	36.0	36.0	36.0	36.0
7	34.987485898819294	36.0	36.0	36.0	36.0	36.0
8	34.927225645644576	36.0	36.0	36.0	36.0	36.0
9	35.022887108065206	36.0	36.0	36.0	36.0	36.0
10	34.88059727696725	36.0	36.0	36.0	32.0	36.0
11	35.058596258115145	36.0	36.0	36.0	36.0	36.0
12	34.953208325158734	36.0	36.0	36.0	36.0	36.0
13	35.00822773239638	36.0	36.0	36.0	36.0	36.0
14	34.94718271786046	36.0	36.0	36.0	32.0	36.0
15	34.91863758070557	36.0	36.0	36.0	32.0	36.0
16	34.93319245157666	36.0	36.0	36.0	36.0	36.0
17	34.89829843894913	36.0	36.0	36.0	32.0	36.0
18	34.89936717468075	36.0	36.0	36.0	32.0	36.0
19	34.89813875561818	36.0	36.0	36.0	32.0	36.0
20	34.88675247021474	36.0	36.0	36.0	32.0	36.0
21	34.87830040085254	36.0	36.0	36.0	32.0	36.0
22	34.863842510433756	36.0	36.0	36.0	32.0	36.0
23	34.79867805611044	36.0	36.0	36.0	32.0	36.0
24	34.78165910144824	36.0	36.0	36.0	32.0	36.0
25	34.75627502541557	36.0	36.0	36.0	32.0	36.0
26	34.70454933072341	36.0	36.0	36.0	32.0	36.0
27	34.68724250017836	36.0	36.0	36.0	32.0	36.0
28	34.659551794249836	36.0	36.0	36.0	32.0	36.0
29	34.6252039933652	36.0	36.0	36.0	32.0	36.0
30	34.60179686942641	36.0	36.0	36.0	32.0	36.0
31	34.59736029241279	36.0	36.0	36.0	32.0	36.0
32	34.5755800991653	36.0	36.0	36.0	32.0	36.0
33	34.546000225173	36.0	36.0	36.0	32.0	36.0
34	34.53990522668902	36.0	36.0	36.0	32.0	36.0
35	34.51267294847328	36.0	36.0	36.0	32.0	36.0
36	34.491330003299566	36.0	36.0	36.0	32.0	36.0
37	34.48634331303061	36.0	36.0	36.0	32.0	36.0
38	34.056412525861454	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	23.0
21	78.0
22	289.0
23	855.0
24	2241.0
25	5558.0
26	11635.0
27	22888.0
28	39546.0
29	62595.0
30	94955.0
31	137936.0
32	197213.0
33	318524.0
34	791241.0
35	1902770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98837324997443	18.24583426118981	12.224991004785648	26.540801484050114
2	15.91814292466291	20.114358903474354	36.69890802240137	27.268590149461367
3	18.167169775986302	23.715705705571803	28.541068434757793	29.5760560836841
4	12.315559225459523	15.876586071525411	35.580839868608024	36.227014834407036
5	14.52945530427338	36.502857021830636	32.963600003567095	16.004087670328886
6	34.929194377909596	35.36764162866169	16.222102389820378	13.48106160360835
7	30.81592904126394	30.347411434649846	20.21447806352744	18.62218146055877
8	28.37876402145893	32.89388977914025	18.915237232634293	19.81210896676653
9	27.336975636292898	14.197094592673887	18.22632256899409	40.239607202039124
10	16.0015773420115	26.31627936789273	30.956674650915538	26.725468639180228
11	38.178881772932236	20.904748368253543	21.835470907256603	19.080898951557614
12	25.1905546323496	23.442588092937655	27.92897887909761	23.437878395615133
13	29.445062029954183	19.000029818743837	25.144000844957304	26.410907306344676
14	23.666825328172933	19.70982779838767	24.502027671755723	32.12131920168367
15	25.343472435257187	27.08694687700649	21.838966745737316	25.730613941999003
16	26.158457229645936	25.687432942717404	23.212813688742738	24.94129613889392
17	24.47190242205893	25.913009648997647	24.544359081829207	25.07072884711422
18	25.466732360704857	24.512924038667332	25.728579581936224	24.291764018691588
19	25.850836261325533	24.815932216237428	24.994760826139686	24.338470696297353
20	26.104016551330528	23.991347560105584	24.56709932581865	25.33753656274524
21	27.25095224877855	24.082920608592033	24.049869187715352	24.616257954914072
22	26.124270521066933	24.125746966083845	24.664992173280805	25.08499033956842
23	24.355935675081408	23.89145971192848	25.438496019752836	26.314108593237272
24	25.01242910394521	24.82387458086609	24.90809151387601	25.255604801312693
25	25.176027979433446	24.259339250630514	24.997923836860952	25.566708933075088
26	24.810818342692322	25.33122465757242	25.298758482310813	24.559198517424445
27	25.718046298742486	24.41981658412172	24.50303036383316	25.359106753302633
28	24.745593520368125	24.547981914817722	25.38808901690804	25.318335547906113
29	24.755040754084327	24.638775683099094	25.35197215880716	25.254211404009418
30	24.666420685596062	24.816628914889062	25.57477638760077	24.942174011914105
31	25.46542256723978	24.61308143682671	24.301239120353856	25.620256875579656
32	25.124987738103734	24.615589551972604	24.319046737889703	25.94037597203396
33	24.592821440037095	24.19344590497253	25.182785858243562	26.030946796746807
34	25.51839953271028	24.426254726403656	25.201680325675962	24.853665415210102
35	26.096129922593992	24.22672023257473	25.18373336840979	24.49341647642149
36	24.744785349932226	24.950506527787685	24.799601599843047	25.505106522437043
37	25.8958429942213	24.718589480630662	24.516379663979453	24.86918786116858
38	24.93956137512746	24.349541056602323	25.195221983579646	25.515675584690573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	83.5
2	115.0
3	115.0
4	488.0
5	861.0
6	861.0
7	1139.5
8	1418.0
9	1493.5
10	1569.0
11	1569.0
12	2080.0
13	2591.0
14	3859.0
15	5127.0
16	5127.0
17	8100.0
18	11073.0
19	11073.0
20	13981.0
21	16889.0
22	19254.0
23	21619.0
24	21619.0
25	26894.5
26	32170.0
27	32170.0
28	41512.0
29	50854.0
30	63300.5
31	75747.0
32	75747.0
33	99055.0
34	122363.0
35	122363.0
36	136960.5
37	151558.0
38	172865.0
39	194172.0
40	194172.0
41	206749.0
42	219326.0
43	249010.0
44	278694.0
45	278694.0
46	285932.5
47	293171.0
48	293171.0
49	314735.0
50	336299.0
51	341229.0
52	346159.0
53	346159.0
54	330155.0
55	314151.0
56	314151.0
57	305492.5
58	296834.0
59	265937.5
60	235041.0
61	235041.0
62	222106.0
63	209171.0
64	173356.0
65	137541.0
66	137541.0
67	115600.0
68	93659.0
69	93659.0
70	75301.0
71	56943.0
72	44416.5
73	31890.0
74	31890.0
75	24076.0
76	16262.0
77	16262.0
78	15770.0
79	15278.0
80	11897.0
81	8516.0
82	8516.0
83	7942.5
84	7369.0
85	7369.0
86	4747.5
87	2126.0
88	1708.5
89	1291.0
90	1291.0
91	776.5
92	262.0
93	179.5
94	97.0
95	97.0
96	71.0
97	45.0
98	45.0
99	47.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009280026039808804
2	0.0
3	0.0
4	1.3933973032745952E-4
5	0.0
6	1.9507562245844333E-4
7	3.622832988513947E-4
8	0.002842530498680174
9	0.004068720125561818
10	0.0010311140044232004
11	0.004793286723264608
12	3.0654740672041094E-4
13	1.3933973032745952E-4
14	0.0
15	0.0
16	5.573589213098381E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1147178426196761E-4
22	8.360383819647571E-5
23	1.9507562245844333E-4
24	0.0
25	5.573589213098381E-5
26	5.573589213098381E-5
27	1.1147178426196761E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.7867946065491904E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3588352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.076547755411866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40381645852482	36.732317306639914
2	13.673291225627748	13.147292771650928
3	4.265709112176019	6.152417035266779
4	1.865234211435829	3.5869608656449046
5	1.008829828987182	2.4250527725193116
6	0.5982330051751524	1.725658658530006
7	0.40748884337761926	1.3713459786909161
8	0.2961009941297763	1.1388410867764096
9	0.2193257671757921	0.9489983147657384
>10	1.101061916621978	9.482367930655986
>50	0.07198638853868967	2.426253584254282
>100	0.06537623121832427	6.715211603609493
>500	0.013404910677048788	4.549536246429072
>1k	0.009966259673130539	9.098147827427182
>5k	1.748466609321147E-4	0.4995980171389267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6855	0.191034770278947	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5663	0.15781617856888064	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5312	0.148034529499893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4887	0.1361906524220589	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4738	0.13203832845830063	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4613	0.12855483520011415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4612	0.12852696725404866	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4343	0.12103048976243133	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4301	0.11986003602768068	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	4158	0.11587491974031532	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	4099	0.11423071092245131	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3832	0.10678996932296497	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3755	0.1046441374759221	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3729	0.1039195708782193	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3696	0.10299992865805807	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3601	0.10035247378183634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.7867946065491904E-5	0.0
4	0.0	0.0	0.0	2.7867946065491904E-5	0.0
5	0.0	0.0	0.0	2.7867946065491904E-5	2.7867946065491904E-5
6	0.0	0.0	0.0	2.7867946065491904E-5	8.360383819647571E-5
7	0.0	0.0	0.0	8.360383819647571E-5	8.360383819647571E-5
8	0.0	0.0	0.0	8.360383819647571E-5	8.360383819647571E-5
9	0.0	0.0	0.0	1.1147178426196761E-4	8.360383819647571E-5
10	0.0	0.0	0.0	1.1147178426196761E-4	8.360383819647571E-5
11	0.0	0.0	0.0	1.1147178426196761E-4	8.360383819647571E-5
12	0.0	0.0	0.0	1.3933973032745952E-4	2.7867946065491904E-4
13	0.0	0.0	0.0	1.3933973032745952E-4	3.3441535278590283E-4
14	0.0	0.0	0.0	1.3933973032745952E-4	3.622832988513947E-4
15	0.0	0.0	0.0	1.950756224584433E-4	3.901512449168866E-4
16	0.0	0.0	0.0	2.5081151458942715E-4	4.1801919098237856E-4
17	0.0	0.0	0.0	2.7867946065491904E-4	4.1801919098237856E-4
18	0.0	0.0	0.0	3.0654740672041094E-4	5.016230291788543E-4
19	0.0	0.0	0.0	3.3441535278590283E-4	5.016230291788543E-4
20	0.0	0.0	0.0	4.7375508311336235E-4	6.130948134408219E-4
21	0.0	0.0	0.0	5.294909752443462E-4	6.409627595063138E-4
22	0.0	0.0	0.0	6.130948134408219E-4	6.409627595063138E-4
23	0.0	0.0	0.0	8.081704358992651E-4	6.966986516372976E-4
24	0.0	0.0	0.0	0.0012261896268816437	7.245665977027894E-4
25	0.0	0.0	0.0	0.001421265249340087	7.245665977027894E-4
26	0.0	0.0	0.0	0.0018114164942569736	7.524345437682813E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	1090	0.0	18.788656	29
TCTAGCG	1100	0.0	18.763302	28
TAGCGGC	1165	0.0	17.579084	30
GTATCAA	5625	0.0	16.783325	1
CGCCGGT	1280	0.0	16.37562	7
ATCGTTT	820	0.0	15.999714	29
CGGTCCA	1415	0.0	15.943845	10
CAAGACG	1370	0.0	15.766582	4
AAGACGG	1385	0.0	15.711349	5
AGCGGCG	1350	0.0	15.644165	31
CGCAAGA	1375	0.0	15.592665	2
ACCGTCG	895	0.0	15.196106	8
GCGCAAG	1455	0.0	14.846337	1
CCGGTCC	1545	0.0	14.809416	9
TAGGACG	530	0.0	14.792601	4
ATACCGT	975	0.0	14.769585	6
TACCGTC	945	0.0	14.730718	7
CATCGTT	880	0.0	14.72701	28
TCGTTTA	920	0.0	14.608436	30
GCCGGTC	1455	0.0	14.516015	8
>>END_MODULE