FastQCFastQC Report
Thu 2 Feb 2017
SRR4062924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110523
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3670.3320575807750423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2820.2551505116582069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1950.17643386444450476No Hit
GTCTTAGACCTGGCGCCACTTCACACGACCAGCCAGGC1340.12124173249006993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT1240.11219384200573637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA753.6379788E-1227.769461
GAGGAAT350.00205549222.8558532
GACTTCA350.00205549222.855857
TGCGCGA350.00205549222.8558527
CGAGGAA350.00205549222.8558531
GTGCGCG350.00205549222.8558526
CTAGGAT350.00205549222.855853
GCGAGGA350.00205549222.8558530
GTATCAA2200.022.5748231
CTCATTT453.510249E-421.33212723
CCTTTTG400.00442482320.0260541
CTTAAAG400.00445978419.9988715
GCGCGAG400.00445978419.9988728
CGCGAGG400.00445978419.9988729
CAGGGCA400.00445978419.998879
CACTGGA507.1425026E-419.19891414
ACACCTT450.00880403617.7767726
CTGTGCG450.00880403617.77677224
GGTTTAT550.001342319217.4772831
GCTAGGA550.00135050317.461462
Produced by FastQC (version 0.11.5)