##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062924_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110523
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36243134913095	32.0	32.0	32.0	32.0	32.0
2	31.074418899233645	32.0	32.0	32.0	32.0	32.0
3	31.105733648199923	32.0	32.0	32.0	32.0	32.0
4	31.169340318304787	32.0	32.0	32.0	32.0	32.0
5	31.143680500891218	32.0	32.0	32.0	32.0	32.0
6	34.75858418609701	36.0	36.0	36.0	32.0	36.0
7	34.732951512354894	36.0	36.0	36.0	32.0	36.0
8	34.735086814509195	36.0	36.0	36.0	32.0	36.0
9	34.81526017209088	36.0	36.0	36.0	32.0	36.0
10	34.63799390172181	36.0	36.0	36.0	32.0	36.0
11	34.78051627263103	36.0	36.0	36.0	32.0	36.0
12	34.658369751092536	36.0	36.0	36.0	32.0	36.0
13	34.712340417831584	36.0	36.0	36.0	32.0	36.0
14	34.669417225373905	36.0	36.0	36.0	32.0	36.0
15	34.605855794721464	36.0	36.0	36.0	32.0	36.0
16	34.610379739963626	36.0	36.0	36.0	32.0	36.0
17	34.596464084398725	36.0	36.0	36.0	32.0	36.0
18	34.59075486550311	36.0	36.0	36.0	32.0	36.0
19	34.61208074337468	36.0	36.0	36.0	32.0	36.0
20	34.58528993965057	36.0	36.0	36.0	32.0	36.0
21	34.57539154746071	36.0	36.0	36.0	32.0	36.0
22	34.54313581788406	36.0	36.0	36.0	32.0	36.0
23	34.48359165060666	36.0	36.0	36.0	32.0	36.0
24	34.4731141934258	36.0	36.0	36.0	32.0	36.0
25	34.47306895397338	36.0	36.0	36.0	32.0	36.0
26	34.42392986075296	36.0	36.0	36.0	32.0	36.0
27	34.468716918650415	36.0	36.0	36.0	32.0	36.0
28	34.412068076328005	36.0	36.0	36.0	32.0	36.0
29	34.37238402866372	36.0	36.0	36.0	32.0	36.0
30	34.37623843001004	36.0	36.0	36.0	32.0	36.0
31	34.389258344417	36.0	36.0	36.0	32.0	36.0
32	34.349094758557044	36.0	36.0	36.0	32.0	36.0
33	34.327669353890144	36.0	36.0	36.0	32.0	36.0
34	34.32979560815396	36.0	36.0	36.0	32.0	36.0
35	34.27919075667508	36.0	36.0	36.0	32.0	36.0
36	34.317363806628485	36.0	36.0	36.0	32.0	36.0
37	34.28739719334437	36.0	36.0	36.0	32.0	36.0
38	33.7281561303982	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	2.0
11	0.0
12	0.0
13	0.0
14	5.0
15	20.0
16	21.0
17	50.0
18	51.0
19	52.0
20	82.0
21	88.0
22	143.0
23	212.0
24	373.0
25	527.0
26	841.0
27	1238.0
28	1714.0
29	2307.0
30	3195.0
31	4392.0
32	5920.0
33	8884.0
34	22085.0
35	58320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35095282950511	18.244148974711976	12.344214187377727	25.060684008405186
2	16.640262173979956	19.677533247028364	38.142873955513714	25.53933062347797
3	18.3140139895576	23.718905810281328	29.239623204929828	28.727456995231243
4	12.97129052397282	15.926385030899104	36.039304747514045	35.06301969761403
5	14.557151000244293	36.32728029459931	33.89792169955575	15.217647005600643
6	33.616532305492974	35.8061218027017	17.074274132985895	13.503071758819432
7	30.116808266152745	31.46856310451218	20.216606498194945	18.198022131140124
8	27.689509780858106	33.0495286006406	19.533667505112103	19.727294113389192
9	27.04619894681602	15.43493603083549	19.119270371509746	38.39959465083875
10	15.943432076871572	27.04981813575578	32.60889234722499	24.397857440147664
11	35.6804198335143	22.64115092290988	23.65001809627217	18.028411147303654
12	25.3266377126312	24.081614187477378	28.091748099891422	22.5
13	28.617450636164556	21.307440319982625	25.428483521256762	24.646625522596057
14	23.6495412511989	19.739770897048444	26.232830851083083	30.37785700066957
15	24.94140006335128	27.74062174759039	22.391963437259605	24.926014751798725
16	24.614535451880272	26.88117557638713	24.84889065836621	23.65539831336639
17	23.745701357466064	26.476923076923075	25.425339366515836	24.35203619909502
18	23.159332953301302	26.603147027154193	26.60133735081481	23.636182668729695
19	24.792341519028575	25.994860565699707	26.434607937168607	22.778189978103114
20	25.58067989540071	24.73284653039804	25.72726367889103	23.959209895310224
21	26.303407589713895	25.14251072223529	25.26828208979533	23.285799598255487
22	25.755080620351436	24.745290359941364	25.924283827068894	23.575345192638302
23	25.257652668820178	24.778088438882705	25.930852267072034	24.03340662522508
24	24.139678403054898	25.49158002370805	26.34941318058836	24.019328392648696
25	24.22221417841565	24.87647729535048	26.314408267424394	24.58690025880948
26	23.886445494199197	25.25746140341351	26.4529148793687	24.40317822301859
27	24.37497172329548	25.703298194815183	26.45523232140433	23.466497760485
28	24.585817823179728	25.52230838136429	26.18463793555859	23.707235859897395
29	24.22087088717559	25.591812357476385	25.930249393709055	24.257067361638978
30	25.18729641693811	25.188201230546508	25.02171552660152	24.60278682591386
31	24.013065626724817	25.858902089233528	25.75303793917788	24.37499434486378
32	24.218460174268678	25.95481319954035	25.461685321076015	24.365041305114957
33	24.531972456726116	25.68111693223667	25.543581530443284	24.243329080593938
34	24.46272316782945	24.80024612934459	26.389228221624993	24.34780248120097
35	24.273109814035564	25.79702275915117	25.967150807655763	23.962716619157504
36	24.213412482241264	25.264005646598918	26.429521577428083	24.09306029373173
37	24.089037687191784	25.181197122562548	26.133104103515358	24.596661086730307
38	24.122767332018313	26.08172424401455	25.746937150509417	24.048571273457718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	6.0
2	8.0
3	8.0
4	23.0
5	38.0
6	38.0
7	43.0
8	48.0
9	53.0
10	58.0
11	58.0
12	90.0
13	122.0
14	178.5
15	235.0
16	235.0
17	397.5
18	560.0
19	560.0
20	633.5
21	707.0
22	894.5
23	1082.0
24	1082.0
25	1302.5
26	1523.0
27	1523.0
28	1942.5
29	2362.0
30	3104.0
31	3846.0
32	3846.0
33	4222.5
34	4599.0
35	4599.0
36	5159.0
37	5719.0
38	5809.0
39	5899.0
40	5899.0
41	6486.0
42	7073.0
43	7468.5
44	7864.0
45	7864.0
46	8980.0
47	10096.0
48	10096.0
49	9972.5
50	9849.0
51	10051.0
52	10253.0
53	10253.0
54	9847.0
55	9441.0
56	9441.0
57	8817.0
58	8193.0
59	7771.0
60	7349.0
61	7349.0
62	6437.5
63	5526.0
64	4258.0
65	2990.0
66	2990.0
67	2477.5
68	1965.0
69	1965.0
70	1573.5
71	1182.0
72	1007.0
73	832.0
74	832.0
75	654.5
76	477.0
77	477.0
78	315.5
79	154.0
80	93.0
81	32.0
82	32.0
83	22.0
84	12.0
85	12.0
86	11.5
87	11.0
88	8.5
89	6.0
90	6.0
91	5.5
92	5.0
93	5.5
94	6.0
95	6.0
96	7.0
97	8.0
98	8.0
99	198.5
100	389.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10405074056983614
2	0.056096921002868186
3	0.010857468581200293
4	0.0018095780968667154
5	0.0
6	0.0
7	0.0
8	9.047890484333577E-4
9	9.047890484333577E-4
10	9.047890484333577E-4
11	0.0027143671453000733
12	0.0027143671453000733
13	0.015381413823367083
14	0.004523945242166789
15	0.025334093356134017
16	0.006333523339033504
17	0.02081014811396723
18	0.0054287342906001465
19	0.004523945242166789
20	0.0054287342906001465
21	0.004523945242166789
22	0.004523945242166789
23	0.0054287342906001465
24	0.010857468581200293
25	0.015381413823367083
26	0.019000570017100513
27	0.007238312387466862
28	0.003619156193733431
29	0.013571835726500366
30	0.0027143671453000733
31	0.003619156193733431
32	0.003619156193733431
33	0.0054287342906001465
34	0.010857468581200293
35	0.01628620287180044
36	0.012667046678067008
37	0.007238312387466862
38	0.004523945242166789
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	110523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.619246672638276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.53847188954975	19.00780832948798
2	14.091557445434239	9.474950915194121
3	8.011949296229513	8.080670991558318
4	4.989638560701887	6.709915583181782
5	3.5282719272277094	5.930892212480661
6	2.4571413192668943	4.956434407317934
7	1.945797561697661	4.579137374121223
8	1.4667491993433268	3.94488025116944
9	1.1814732082783863	3.5748215303601962
>10	5.700137255429663	31.084027758928006
>50	0.07535592216809753	1.659383114826778
>100	0.01345641467287456	0.9970775313735603
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	367	0.3320575807750423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	282	0.2551505116582069	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	195	0.17643386444450476	No Hit
GTCTTAGACCTGGCGCCACTTCACACGACCAGCCAGGC	134	0.12124173249006993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	124	0.11219384200573637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.047890484333577E-4	0.0
6	0.0	0.0	0.0	9.047890484333577E-4	0.0
7	0.0	0.0	0.0	9.047890484333577E-4	0.0
8	0.0	0.0	0.0	9.047890484333577E-4	0.0
9	0.0	0.0	0.0	9.047890484333577E-4	0.0
10	0.0	0.0	0.0	9.047890484333577E-4	0.0
11	0.0	0.0	0.0	0.0018095780968667154	0.0
12	0.0	0.0	0.0	0.0018095780968667154	0.0
13	0.0	0.0	0.0	0.0018095780968667154	0.0
14	0.0	0.0	0.0	0.0018095780968667154	0.0
15	0.0	0.0	0.0	0.0018095780968667154	0.0
16	0.0	0.0	0.0	0.0018095780968667154	0.0
17	0.0	0.0	0.0	0.0018095780968667154	0.0
18	0.0	0.0	0.0	0.0018095780968667154	0.0
19	0.0	0.0	0.0	0.0018095780968667154	0.0
20	0.0	0.0	0.0	0.0018095780968667154	0.0
21	0.0	0.0	0.0	0.003619156193733431	0.0
22	0.0	0.0	0.0	0.004523945242166789	0.0
23	0.0	0.0	0.0	0.004523945242166789	0.0
24	0.0	0.0	0.0	0.004523945242166789	0.0
25	0.0	0.0	0.0	0.0054287342906001465	0.0
26	0.0	0.0	0.0	0.0054287342906001465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	75	3.6379788E-12	27.76946	1
GAGGAAT	35	0.002055492	22.85585	32
GACTTCA	35	0.002055492	22.85585	7
TGCGCGA	35	0.002055492	22.85585	27
CGAGGAA	35	0.002055492	22.85585	31
GTGCGCG	35	0.002055492	22.85585	26
CTAGGAT	35	0.002055492	22.85585	3
GCGAGGA	35	0.002055492	22.85585	30
GTATCAA	220	0.0	22.574823	1
CTCATTT	45	3.510249E-4	21.332127	23
CCTTTTG	40	0.004424823	20.026054	1
CTTAAAG	40	0.004459784	19.99887	15
GCGCGAG	40	0.004459784	19.99887	28
CGCGAGG	40	0.004459784	19.99887	29
CAGGGCA	40	0.004459784	19.99887	9
CACTGGA	50	7.1425026E-4	19.198914	14
ACACCTT	45	0.008804036	17.776772	6
CTGTGCG	45	0.008804036	17.776772	24
GGTTTAT	55	0.0013423192	17.477283	1
GCTAGGA	55	0.001350503	17.46146	2
>>END_MODULE