FastQCFastQC Report
Thu 2 Feb 2017
SRR4062924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110523
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3330.30129475312830817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT2120.19181527826787184No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT1430.12938483392597014No Hit
CCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAG1360.12305131058693666No Hit
GTCTTAGACCTGGCGCCACTTCACACGACCAGCCAGGC1260.11400342010260307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCT200.003738970532.05
GGTATCA601.8189894E-1029.3333341
CACTCAG350.002054816822.85714323
GTATCAA1900.022.7368411
GTACATT400.00445832520.09
GTCTCCA400.00445832520.01
CCACTCA400.00445832520.022
GTTCATA400.00445832520.01
AAAACAA450.008801172517.77777725
CAGGGTG450.008801172517.7777775
ATAATCA550.001354097917.4545464
ATACTGA550.001354097917.4545466
TATCAAC3050.015.2131152
TCAACGC2900.014.8965524
AACGCAG3000.014.46
CAACGCA3050.014.1639355
TAATCAA700.00675269113.7142865
ATCAACG3150.013.7142863
TCCAGCT700.00675269113.7142864
GAGTACT952.3959907E-413.47368412
Produced by FastQC (version 0.11.5)