##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062924_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110523
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26019018665798	32.0	32.0	32.0	32.0	32.0
2	31.40113822462293	32.0	32.0	32.0	32.0	32.0
3	31.441871827583398	32.0	32.0	32.0	32.0	32.0
4	31.519059381305247	32.0	32.0	32.0	32.0	32.0
5	31.46460917637053	32.0	32.0	32.0	32.0	32.0
6	34.9878124915176	36.0	36.0	36.0	36.0	36.0
7	35.00898455525094	36.0	36.0	36.0	36.0	36.0
8	34.96481275390643	36.0	36.0	36.0	36.0	36.0
9	35.06024085484469	36.0	36.0	36.0	36.0	36.0
10	34.95197379730916	36.0	36.0	36.0	32.0	36.0
11	35.06250282746578	36.0	36.0	36.0	36.0	36.0
12	35.00123051310587	36.0	36.0	36.0	36.0	36.0
13	35.002071966920916	36.0	36.0	36.0	36.0	36.0
14	34.97324538783783	36.0	36.0	36.0	36.0	36.0
15	34.94939514852112	36.0	36.0	36.0	36.0	36.0
16	34.98555051889652	36.0	36.0	36.0	36.0	36.0
17	34.94026582702243	36.0	36.0	36.0	36.0	36.0
18	34.922215285506184	36.0	36.0	36.0	36.0	36.0
19	34.95381051907748	36.0	36.0	36.0	36.0	36.0
20	34.91958234937524	36.0	36.0	36.0	36.0	36.0
21	34.93353419650209	36.0	36.0	36.0	36.0	36.0
22	34.92080381459063	36.0	36.0	36.0	32.0	36.0
23	34.83768084471106	36.0	36.0	36.0	32.0	36.0
24	34.839173746640974	36.0	36.0	36.0	32.0	36.0
25	34.849660251712315	36.0	36.0	36.0	32.0	36.0
26	34.76348814273952	36.0	36.0	36.0	32.0	36.0
27	34.77330510391502	36.0	36.0	36.0	32.0	36.0
28	34.73042715090977	36.0	36.0	36.0	32.0	36.0
29	34.697465685875336	36.0	36.0	36.0	32.0	36.0
30	34.678917510382455	36.0	36.0	36.0	32.0	36.0
31	34.68659916940366	36.0	36.0	36.0	32.0	36.0
32	34.65982646146051	36.0	36.0	36.0	32.0	36.0
33	34.63334328601287	36.0	36.0	36.0	32.0	36.0
34	34.604272413886704	36.0	36.0	36.0	32.0	36.0
35	34.597540783366355	36.0	36.0	36.0	32.0	36.0
36	34.574948200826974	36.0	36.0	36.0	32.0	36.0
37	34.540159061914714	36.0	36.0	36.0	32.0	36.0
38	34.19894501596953	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	9.0
23	17.0
24	82.0
25	175.0
26	387.0
27	643.0
28	1285.0
29	1951.0
30	2904.0
31	4052.0
32	5678.0
33	8840.0
34	21912.0
35	62585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76944864219204	19.378512157380847	12.884018496231075	25.968020704196036
2	15.541561484939786	20.91600843263393	37.518887471386044	26.023542611040234
3	17.137609366376232	24.720646381296202	29.361309410710895	28.780434841616675
4	12.079838585633759	16.79469431701999	35.816979271282904	35.30848782606335
5	13.621599124164202	37.0158247604571	33.92597016005718	15.436605955321516
6	33.12975579743583	36.24313491309501	17.136704577327798	13.490404712141366
7	29.820037458266608	31.540041439338417	20.16322394433738	18.476697158057597
8	27.49834875998661	33.5468635487636	19.38239099553939	19.572396695710395
9	26.98415571018794	15.128536914209187	18.941662971777077	38.945644403825796
10	15.376889878124917	27.686544882060748	32.41587723822191	24.52068800159243
11	35.63582402866501	22.601748131526087	23.427857905499557	18.334569934309343
12	24.702550600327534	24.07100784452105	28.146177718664894	23.080263836486523
13	28.14255856247116	21.641649249477485	25.41281000334772	24.802982184703637
14	23.364367597694596	19.94788415081024	26.08687784443057	30.600870407064594
15	24.501687431575327	28.024935986174825	22.28947820815577	25.18389837409408
16	24.27820453661229	26.831519231291225	24.66183509314803	24.228441138948455
17	23.55075414167187	26.855043746550493	25.192946264578413	24.401255847199224
18	22.81968459053772	26.944617862345392	26.414411479963444	23.821286067153444
19	24.765885833717867	26.023542611040234	26.113116726835138	23.097454828406757
20	25.320521520407517	24.984844783438742	25.40014295666965	24.29449073948409
21	25.66072220261846	25.535861313934653	25.362141816635454	23.44127466681143
22	25.678817983587127	24.849126426173736	25.696913764555795	23.775141825683342
23	24.902508980031307	25.005654931552705	25.766582521285166	24.325253567130822
24	23.967861892999647	25.8516326918379	26.071496430607205	24.10900898455525
25	23.76156998995684	25.139563710720847	26.240691982664245	24.858174316658072
26	24.013101345421315	25.667055725957493	25.861585371370666	24.458257557250526
27	24.22413637103925	26.261739744123343	25.96496625106314	23.54915763377427
28	24.080960524053815	25.640816843552926	25.751201107461796	24.52702152493146
29	23.897288347221846	26.09230657872117	25.599196547324993	24.411208526731993
30	25.05270396207124	25.436334518606984	24.7722193570569	24.738742162264867
31	23.62675642174027	25.766582521285166	25.905015245695466	24.7016458112791
32	24.374112175746223	25.883300308533062	25.384761542846284	24.357825972874426
33	24.416637261022593	25.750296318413362	25.419143526686756	24.41392289387729
34	24.022149235905648	24.84188811378627	26.465984455724147	24.669978194583933
35	24.24020339657809	25.64172163260136	26.05159107154167	24.066483899278886
36	24.007672611130715	25.66615093690906	26.172832804031742	24.153343647928484
37	23.987767252065183	25.278901224179585	26.190023795951973	24.543307727803263
38	24.021244446857214	26.07240121965564	25.59829175827656	24.30806257521059
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	3.0
4	18.0
5	33.0
6	33.0
7	39.0
8	45.0
9	54.0
10	63.0
11	63.0
12	89.0
13	115.0
14	179.5
15	244.0
16	244.0
17	379.5
18	515.0
19	515.0
20	645.0
21	775.0
22	900.0
23	1025.0
24	1025.0
25	1306.0
26	1587.0
27	1587.0
28	1973.5
29	2360.0
30	3075.5
31	3791.0
32	3791.0
33	4177.5
34	4564.0
35	4564.0
36	5184.5
37	5805.0
38	5896.0
39	5987.0
40	5987.0
41	6464.5
42	6942.0
43	7456.5
44	7971.0
45	7971.0
46	8855.5
47	9740.0
48	9740.0
49	9924.5
50	10109.0
51	10210.0
52	10311.0
53	10311.0
54	9947.5
55	9584.0
56	9584.0
57	8892.5
58	8201.0
59	7827.0
60	7453.0
61	7453.0
62	6571.0
63	5689.0
64	4356.0
65	3023.0
66	3023.0
67	2519.5
68	2016.0
69	2016.0
70	1610.0
71	1204.0
72	967.0
73	730.0
74	730.0
75	573.5
76	417.0
77	417.0
78	296.5
79	176.0
80	100.0
81	24.0
82	24.0
83	16.5
84	9.0
85	9.0
86	6.5
87	4.0
88	3.5
89	3.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	2.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012667046678067008
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.009047890484333578
10	0.0
11	0.004523945242166789
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	9.047890484333577E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	110523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.05305682980013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.8982668096878	17.276042090786532
2	14.867611358888952	9.53104783619699
3	8.335685654603964	8.015526180071117
4	5.41410263648168	6.94154157958072
5	3.723254107152939	5.967083774417994
6	2.839722237904364	5.4613066963437475
7	1.9928865804776152	4.4714674773576535
8	1.4650256873482754	3.7566841290953015
9	1.1827471348726923	3.411959501642192
>10	6.1706091571162425	32.3425893252988
>50	0.09597470784169819	1.965201813197253
>100	0.014113927623779146	0.8595495960116899
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	333	0.30129475312830817	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	212	0.19181527826787184	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT	143	0.12938483392597014	No Hit
CCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAG	136	0.12305131058693666	No Hit
GTCTTAGACCTGGCGCCACTTCACACGACCAGCCAGGC	126	0.11400342010260307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.047890484333577E-4	0.0
6	0.0	0.0	0.0	9.047890484333577E-4	0.0
7	0.0	0.0	0.0	9.047890484333577E-4	0.0
8	0.0	0.0	0.0	9.047890484333577E-4	0.0
9	0.0	0.0	0.0	9.047890484333577E-4	0.0
10	0.0	0.0	0.0	9.047890484333577E-4	0.0
11	0.0	0.0	0.0	0.0018095780968667154	0.0
12	0.0	0.0	0.0	0.0018095780968667154	0.0
13	0.0	0.0	0.0	0.0018095780968667154	0.0
14	0.0	0.0	0.0	0.0018095780968667154	0.0
15	0.0	0.0	0.0	0.0018095780968667154	0.0
16	0.0	0.0	0.0	0.0018095780968667154	0.0
17	0.0	0.0	0.0	0.0018095780968667154	0.0
18	0.0	0.0	0.0	0.0018095780968667154	0.0
19	0.0	0.0	0.0	0.0018095780968667154	0.0
20	0.0	0.0	0.0	0.0018095780968667154	0.0
21	0.0	0.0	0.0	0.003619156193733431	0.0
22	0.0	0.0	0.0	0.004523945242166789	0.0
23	0.0	0.0	0.0	0.004523945242166789	0.0
24	0.0	0.0	0.0	0.004523945242166789	0.0
25	0.0	0.0	0.0	0.0054287342906001465	0.0
26	0.0	0.0	0.0	0.0054287342906001465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCT	20	0.0037389705	32.0	5
GGTATCA	60	1.8189894E-10	29.333334	1
CACTCAG	35	0.0020548168	22.857143	23
GTATCAA	190	0.0	22.736841	1
GTACATT	40	0.004458325	20.0	9
GTCTCCA	40	0.004458325	20.0	1
CCACTCA	40	0.004458325	20.0	22
GTTCATA	40	0.004458325	20.0	1
AAAACAA	45	0.0088011725	17.777777	25
CAGGGTG	45	0.0088011725	17.777777	5
ATAATCA	55	0.0013540979	17.454546	4
ATACTGA	55	0.0013540979	17.454546	6
TATCAAC	305	0.0	15.213115	2
TCAACGC	290	0.0	14.896552	4
AACGCAG	300	0.0	14.4	6
CAACGCA	305	0.0	14.163935	5
TAATCAA	70	0.006752691	13.714286	5
ATCAACG	315	0.0	13.714286	3
TCCAGCT	70	0.006752691	13.714286	4
GAGTACT	95	2.3959907E-4	13.473684	12
>>END_MODULE