FastQCFastQC Report
Thu 2 Feb 2017
SRR4062923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651127
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT90510.3414019773477468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT53420.201499211467425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT30370.1145550552651759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3450.018.0810414
ACCGTCG4400.017.089838
GTATCAA64000.016.4879591
CGTCGTA4900.016.000510
TCTAGCG5700.015.71919628
CTAGCGG5800.015.4490529
TACCGTC5000.015.3590317
CCGTCGT5050.015.2083969
TAGCGGC6150.014.82973230
AGGACGT4650.014.7916965
GTCCTAT6200.014.4629791
AGGCCCG6650.014.43654110
ATACCGT5550.014.4105156
TCGTTTA5000.014.080440530
GTCCTAC7200.014.0110121
AACGCAG74650.013.9279126
CGTTTAT5200.013.84658631
ATCGTTT5250.013.71497329
TAATACG700.006821635613.70984
CGGTCCA6850.013.5478710
Produced by FastQC (version 0.11.5)