FastQCFastQC Report
Thu 2 Feb 2017
SRR4062923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651127
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT94960.3581872916687884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT60150.22688464189003393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT38070.1435993070116973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4850.019.13333329
TAGCGGC5150.018.32943730
TCTAGCG5100.018.19542328
TACGTTC550.001364404717.4562237
ACGTTTT3500.016.4565529
TACGCTA5450.015.5608369
GTATCAA60300.015.52329351
CACGTTT3550.015.32339228
GTCCTAC6300.015.2389851
TACCGTC5350.014.9547077
ATACGCT5800.014.8979848
ACGCTAT5650.014.72596810
ATACCGT5550.014.7041166
ACCGTCG5450.014.680318
AGCGGCG6350.014.61364731
CGTCGTA5150.014.60224210
TAGCGTA3650.014.4671447
ATAGCGT3850.014.1312276
GTATTAG4900.014.04163551
AATAGCG3900.013.9500595
Produced by FastQC (version 0.11.5)