##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2651127
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168935701684603	32.0	32.0	32.0	32.0	32.0
2	31.27046271265013	32.0	32.0	32.0	32.0	32.0
3	31.36697525241152	32.0	32.0	32.0	32.0	32.0
4	31.468783275942645	32.0	32.0	32.0	32.0	32.0
5	31.38590946416373	32.0	32.0	32.0	32.0	32.0
6	34.912596416542854	36.0	36.0	36.0	36.0	36.0
7	34.94248144279772	36.0	36.0	36.0	36.0	36.0
8	34.888740147114795	36.0	36.0	36.0	32.0	36.0
9	34.99114301200961	36.0	36.0	36.0	36.0	36.0
10	34.83808621767271	36.0	36.0	36.0	32.0	36.0
11	35.02167644175477	36.0	36.0	36.0	36.0	36.0
12	34.92302632050445	36.0	36.0	36.0	32.0	36.0
13	34.972187299967146	36.0	36.0	36.0	36.0	36.0
14	34.92096455582852	36.0	36.0	36.0	32.0	36.0
15	34.890232719896105	36.0	36.0	36.0	32.0	36.0
16	34.90409625793106	36.0	36.0	36.0	32.0	36.0
17	34.87033703025166	36.0	36.0	36.0	32.0	36.0
18	34.873767646740426	36.0	36.0	36.0	32.0	36.0
19	34.86676043810802	36.0	36.0	36.0	32.0	36.0
20	34.85363281351667	36.0	36.0	36.0	32.0	36.0
21	34.84238438973312	36.0	36.0	36.0	32.0	36.0
22	34.83047511492283	36.0	36.0	36.0	32.0	36.0
23	34.774207723734094	36.0	36.0	36.0	32.0	36.0
24	34.75954377138477	36.0	36.0	36.0	32.0	36.0
25	34.744057527232755	36.0	36.0	36.0	32.0	36.0
26	34.67777364117222	36.0	36.0	36.0	32.0	36.0
27	34.673221992005665	36.0	36.0	36.0	32.0	36.0
28	34.63477947303166	36.0	36.0	36.0	32.0	36.0
29	34.60537763751039	36.0	36.0	36.0	32.0	36.0
30	34.58977559354946	36.0	36.0	36.0	32.0	36.0
31	34.5797689058276	36.0	36.0	36.0	32.0	36.0
32	34.55568858074321	36.0	36.0	36.0	32.0	36.0
33	34.515776498070444	36.0	36.0	36.0	32.0	36.0
34	34.50889225601037	36.0	36.0	36.0	32.0	36.0
35	34.48661380612849	36.0	36.0	36.0	32.0	36.0
36	34.463290517579885	36.0	36.0	36.0	32.0	36.0
37	34.456374968079615	36.0	36.0	36.0	32.0	36.0
38	34.04070759341216	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	15.0
21	47.0
22	189.0
23	614.0
24	1698.0
25	4284.0
26	8992.0
27	17265.0
28	30342.0
29	47472.0
30	71823.0
31	104171.0
32	151256.0
33	242798.0
34	600860.0
35	1369298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81362108574856	18.43867531281947	12.281950590179147	26.465753011252822
2	15.691213585769374	20.662721929202185	37.3127730206814	26.33329146434705
3	18.13821065531753	24.628431606633708	28.650683275452288	28.582674462596476
4	12.136286344961608	16.529064653331947	36.355862645428886	34.97878635627757
5	14.071196190296572	36.98814654156254	33.31936442076477	15.621292847376115
6	33.50737291885206	36.346964171005396	16.781618040066824	13.364044870075714
7	29.911154463890327	30.833482327078617	20.63552056151405	18.61984264751701
8	27.735488932812284	33.11348582464505	19.242271074430043	19.90875416811262
9	27.467924646447806	14.30074352429232	18.284941322651672	39.9463905066082
10	15.39174160734217	26.908823026845113	31.923728177429943	25.775707188382775
11	37.1293334102854	21.451368747126086	22.512515083924903	18.906782758663613
12	25.071517051686914	23.823099404175448	28.76720596156487	22.338177582572776
13	29.483973784714035	19.89329058418596	25.171691263143003	25.451044367957
14	23.473790580383362	19.89915986672838	25.53906319840581	31.08798635448245
15	24.9703993810934	27.40381731995487	22.558594891908236	25.067188407043496
16	25.325814512706867	26.24810222075534	23.834862558347872	24.59122070818992
17	23.814287282351994	26.013427497060682	25.290376507802154	24.881908712785165
18	24.464916241281536	25.213805298652233	26.50208760274404	23.819190857322187
19	25.312140836708313	24.905068674567456	25.9275394954674	23.85525099325683
20	25.744447550041926	24.36212976594482	25.510848782423473	24.382573901589776
21	26.667076555859538	24.285746078171403	25.00419633491166	24.042981031057405
22	25.6350794474044	24.497260596916405	25.53184025649489	24.33581969918431
23	24.3448153857818	24.421537589919442	25.890650867575744	25.342996156723018
24	24.51651693789094	25.158394901489068	25.766626796830177	24.558461363789817
25	24.624753642321657	24.710151350841624	25.864868687821208	24.80022631901551
26	24.380640060593166	25.62026521581035	25.990975903050934	24.008118820545548
27	24.89230027599646	25.01066529969775	25.528974347078087	24.568060077227706
28	24.189599366608995	25.11248989580658	26.006449332680027	24.691461404904405
29	24.230299038861585	25.070658629330094	25.976537525361852	24.722504806446466
30	24.184884390676118	25.272949956754243	26.07879592339409	24.463369729175554
31	24.86470848058203	25.029129121313314	25.2368898208196	24.869272577285056
32	24.472233883929363	25.18159258307882	25.201282322574514	25.144891210417303
33	24.177755347065606	24.924909293293005	25.710084805443117	25.187250554198272
34	24.770031763849865	25.082200890413773	25.86186931067429	24.28589803506207
35	25.19826473797747	24.83589054768029	25.829656595100875	24.136188119241364
36	24.234938575179537	25.495081902903934	25.56905044533891	24.700929076577623
37	25.023772908653562	25.225837917232933	25.336281513484643	24.414107660628854
38	24.30760526191711	25.19179593568768	25.696261021264554	24.804337781130652
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	115.5
2	173.0
3	173.0
4	551.5
5	930.0
6	930.0
7	1271.0
8	1612.0
9	1748.0
10	1884.0
11	1884.0
12	2661.0
13	3438.0
14	4869.5
15	6301.0
16	6301.0
17	9687.5
18	13074.0
19	13074.0
20	16226.0
21	19378.0
22	20280.0
23	21182.0
24	21182.0
25	25446.0
26	29710.0
27	29710.0
28	38456.0
29	47202.0
30	58180.5
31	69159.0
32	69159.0
33	84761.5
34	100364.0
35	100364.0
36	110483.0
37	120602.0
38	137102.5
39	153603.0
40	153603.0
41	164014.0
42	174425.0
43	193601.0
44	212777.0
45	212777.0
46	215462.0
47	218147.0
48	218147.0
49	229342.0
50	240537.0
51	241343.0
52	242149.0
53	242149.0
54	234073.5
55	225998.0
56	225998.0
57	215987.0
58	205976.0
59	184169.0
60	162362.0
61	162362.0
62	150247.5
63	138133.0
64	115507.0
65	92881.0
66	92881.0
67	77044.5
68	61208.0
69	61208.0
70	49101.0
71	36994.0
72	28772.5
73	20551.0
74	20551.0
75	15657.0
76	10763.0
77	10763.0
78	9822.0
79	8881.0
80	6730.5
81	4580.0
82	4580.0
83	4278.5
84	3977.0
85	3977.0
86	2556.5
87	1136.0
88	940.0
89	744.0
90	744.0
91	432.5
92	121.0
93	90.5
94	60.0
95	60.0
96	45.0
97	30.0
98	30.0
99	28.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008939594368734505
2	0.0
3	0.0
4	1.1315942238904436E-4
5	7.543961492602956E-5
6	2.2631884477808872E-4
7	3.394782671671331E-4
8	0.001772830950761695
9	0.003922859976153537
10	0.0011315942238904436
11	0.0047526957403398636
12	4.1491788209316267E-4
13	7.543961492602956E-5
14	0.0
15	0.0
16	7.543961492602956E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5087922985205913E-4
22	7.543961492602956E-5
23	1.5087922985205913E-4
24	0.0
25	7.543961492602956E-5
26	1.1315942238904436E-4
27	2.2631884477808872E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.543961492602956E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2651127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.87831732258235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.9212656887904	32.30912963233123
2	16.604096589813285	15.567442175841489
3	6.328661622838188	8.900310233479725
4	2.9754548528651013	5.579372670865112
5	1.576092588336918	3.6942284242914085
6	0.9147760084874935	2.5729896002977206
7	0.6006946041314738	1.971168658650675
8	0.3985813000293841	1.4947856529299894
9	0.27396910569391986	1.155888960597331
>10	1.2481350422885693	10.225794817667493
>50	0.0763447984399736	2.4813570468847876
>100	0.0668037880022138	6.720651398807963
>500	0.010028755481817907	3.2186166466810793
>1k	0.004933500680563961	3.520183051535953
>5k	1.6175412067422822E-4	0.5880810291379815
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9496	0.3581872916687884	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6015	0.22688464189003393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3807	0.1435993070116973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.640386522411035E-4
2	0.0	0.0	0.0	0.0	2.640386522411035E-4
3	0.0	0.0	0.0	0.0	2.640386522411035E-4
4	0.0	0.0	0.0	0.0	3.017584597041183E-4
5	0.0	0.0	0.0	0.0	3.017584597041183E-4
6	0.0	0.0	0.0	0.0	4.149178820931626E-4
7	0.0	0.0	0.0	0.0	4.149178820931626E-4
8	0.0	0.0	0.0	0.0	4.903574970191923E-4
9	0.0	0.0	0.0	0.0	4.903574970191923E-4
10	0.0	0.0	0.0	0.0	5.28077304482207E-4
11	0.0	0.0	0.0	0.0	5.28077304482207E-4
12	0.0	0.0	0.0	0.0	0.001056154608964414
13	0.0	0.0	0.0	0.0	0.0011693140313534583
14	0.0	0.0	0.0	0.0	0.0011693140313534583
15	0.0	0.0	0.0	3.771980746301479E-5	0.001433352683594562
16	0.0	0.0	0.0	3.771980746301479E-5	0.0015465121059836061
17	0.0	0.0	0.0	7.543961492602958E-5	0.0015465121059836061
18	0.0	0.0	0.0	1.8859903731507392E-4	0.0015465121059836061
19	0.0	0.0	0.0	2.2631884477808872E-4	0.001584231913446621
20	0.0	0.0	0.0	2.640386522411035E-4	0.0018105507582247098
21	0.0	0.0	0.0	3.394782671671331E-4	0.002074589410465813
22	0.0	0.0	0.0	5.28077304482207E-4	0.002112309217928828
23	3.771980746301479E-5	0.0	0.0	9.052753791123549E-4	0.0021500290253918426
24	3.771980746301479E-5	0.0	0.0	0.0016219517209096358	0.0021500290253918426
25	3.771980746301479E-5	0.0	0.0	0.001961429988076769	0.0021500290253918426
26	3.771980746301479E-5	0.0	0.0	0.0024140676776329465	0.0023763478701699313
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	485	0.0	19.133333	29
TAGCGGC	515	0.0	18.329437	30
TCTAGCG	510	0.0	18.195423	28
TACGTTC	55	0.0013644047	17.456223	7
ACGTTTT	350	0.0	16.45655	29
TACGCTA	545	0.0	15.560836	9
GTATCAA	6030	0.0	15.5232935	1
CACGTTT	355	0.0	15.323392	28
GTCCTAC	630	0.0	15.238985	1
TACCGTC	535	0.0	14.954707	7
ATACGCT	580	0.0	14.897984	8
ACGCTAT	565	0.0	14.725968	10
ATACCGT	555	0.0	14.704116	6
ACCGTCG	545	0.0	14.68031	8
AGCGGCG	635	0.0	14.613647	31
CGTCGTA	515	0.0	14.602242	10
TAGCGTA	365	0.0	14.467144	7
ATAGCGT	385	0.0	14.131227	6
GTATTAG	490	0.0	14.0416355	1
AATAGCG	390	0.0	13.950059	5
>>END_MODULE