FastQCFastQC Report
Thu 2 Feb 2017
SRR4062922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931074
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT122880.41923199482510504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT79630.2716751607090097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT43780.1493650450312752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.12125248287828967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG3150.018.283917
ATACGAC550.001366857417.4516333
GATATAC7400.016.67091
AGAACCG4650.016.167695
CGCGGTC3900.015.17980410
ATCGTTT5500.014.544764529
GTCCTAA11750.014.4533351
GTCCTAC19750.014.2772851
TTAGGAC14250.014.2572693
GAACCGC4550.014.0621476
ACGTTTT9350.014.03142129
TATACAC11450.013.9714673
CTAGCGG4600.013.91238329
TAGGACG20900.013.8527294
TAGGACA7100.013.7427784
GCATCGT5850.013.67339827
TCACGTT9950.013.66652827
CACGTTT9850.013.64309128
CATCGTT5750.013.63320528
CGTTATT3650.013.5965162
Produced by FastQC (version 0.11.5)