##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062922_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2931074
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210648042321687	32.0	32.0	32.0	32.0	32.0
2	30.878313546502067	32.0	32.0	32.0	32.0	32.0
3	30.92671730567021	32.0	32.0	32.0	32.0	32.0
4	30.982045489127877	32.0	32.0	32.0	32.0	32.0
5	30.93806195271767	32.0	32.0	32.0	32.0	32.0
6	34.576794717567694	36.0	36.0	36.0	32.0	36.0
7	34.48848749639211	36.0	36.0	36.0	32.0	36.0
8	34.47665804411625	36.0	36.0	36.0	32.0	36.0
9	34.58557648152179	36.0	36.0	36.0	32.0	36.0
10	34.3525226589298	36.0	36.0	36.0	32.0	36.0
11	34.59557895842957	36.0	36.0	36.0	32.0	36.0
12	34.442920581329574	36.0	36.0	36.0	32.0	36.0
13	34.508144454899465	36.0	36.0	36.0	32.0	36.0
14	34.42412644648344	36.0	36.0	36.0	32.0	36.0
15	34.388946509027065	36.0	36.0	36.0	32.0	36.0
16	34.394177356149996	36.0	36.0	36.0	32.0	36.0
17	34.33755203723959	36.0	36.0	36.0	32.0	36.0
18	34.36686245383092	36.0	36.0	36.0	32.0	36.0
19	34.33771989380002	36.0	36.0	36.0	32.0	36.0
20	34.30964520172469	36.0	36.0	36.0	32.0	36.0
21	34.28845672268936	36.0	36.0	36.0	32.0	36.0
22	34.271938204221385	36.0	36.0	36.0	32.0	36.0
23	34.23597766552465	36.0	36.0	36.0	32.0	36.0
24	34.22351875114719	36.0	36.0	36.0	32.0	36.0
25	34.207710211342324	36.0	36.0	36.0	32.0	36.0
26	34.17017687032126	36.0	36.0	36.0	32.0	36.0
27	34.1768324511766	36.0	36.0	36.0	32.0	36.0
28	34.14975500447959	36.0	36.0	36.0	32.0	36.0
29	34.114942509128056	36.0	36.0	36.0	32.0	36.0
30	34.07443721994054	36.0	36.0	36.0	32.0	36.0
31	34.092192486440126	36.0	36.0	36.0	32.0	36.0
32	34.05510232767921	36.0	36.0	36.0	32.0	36.0
33	34.01872590047198	36.0	36.0	36.0	32.0	36.0
34	34.010003159251525	36.0	36.0	36.0	32.0	36.0
35	33.97589313678194	36.0	36.0	36.0	32.0	36.0
36	33.926306534737776	36.0	36.0	36.0	32.0	36.0
37	33.923039472903106	36.0	36.0	36.0	32.0	36.0
38	33.33296634612432	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	8.0
8	7.0
9	8.0
10	29.0
11	40.0
12	15.0
13	11.0
14	560.0
15	965.0
16	1272.0
17	1486.0
18	1915.0
19	2302.0
20	3031.0
21	4181.0
22	5971.0
23	8720.0
24	12482.0
25	18769.0
26	27084.0
27	38852.0
28	54101.0
29	73250.0
30	98525.0
31	132406.0
32	179909.0
33	270898.0
34	611662.0
35	1382611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10608500436903	18.556848234592437	12.092397894942518	25.244668866096013
2	15.349945927936687	20.554894067912326	39.96038128997373	24.13477871417726
3	18.39705273185988	25.39466491783231	29.458179815567124	26.750102534740684
4	11.93266416011027	16.52904489283448	37.98541784659056	33.552873100464694
5	13.406376105910883	37.69349602261019	34.569242337605274	14.33088553387366
6	31.56716074191463	37.505718911044276	17.973842272348108	12.953278074692987
7	28.502506932617145	31.835417212542033	21.76708726363597	17.894988591204854
8	26.60067682068278	34.598781258774565	20.221647003111848	18.57889491743081
9	27.28673088457358	14.972798255376379	19.811112832140708	37.92935802790934
10	15.481758110387519	27.42731629828976	32.93973230125934	24.15119329006338
11	35.21494370522006	22.203411804844762	24.319208461276016	18.26243602865916
12	24.654151866455727	24.695776126985383	30.055854981076312	20.59421702548258
13	28.653176433334448	21.005802879533096	26.219420230709332	24.121600456423124
14	22.519965633792435	21.524908335170156	26.733408716978996	29.221717314058417
15	23.896839767482017	29.073347452219085	23.629909560414887	23.399903219884013
16	23.793538789103017	27.06604520390894	25.93086340557952	23.20955260140853
17	22.246644021618582	27.21077571233281	27.007325174837106	23.535255091211496
18	22.769676733888385	26.07525065696589	28.943279500003072	22.211793109142658
19	24.485958476793197	25.8385386047632	27.0822422081292	22.593260710314407
20	24.502447204693446	25.999361969637263	26.939757309627264	22.55843351604203
21	25.302188119376034	25.22292845851731	26.063155926578872	23.411727495527785
22	24.266330837016277	26.255445943857264	26.564844135713482	22.913379083412973
23	22.968416950976547	26.123173311141297	27.267066355350334	23.641343382531822
24	23.516338928131372	26.52384002260258	27.204305955117636	22.75551509414841
25	23.536182212522128	25.995306010190554	27.47587829749713	22.992633479790182
26	22.58596738236909	26.8762235535835	28.097550555348725	22.440258508698683
27	23.24780597223213	26.861055201025525	26.85808672521502	23.033052101527332
28	22.51255845914539	26.70626324468345	27.29451399283427	23.4866643033369
29	23.018237392164497	26.316418020450143	27.04125276338341	23.62409182400195
30	22.842309147589074	26.50171325592321	27.533692782148155	23.122284814339558
31	23.598549520077846	26.060564497088258	26.706101760146737	23.634784222687156
32	22.661178415806653	26.955914410309305	26.796882970124543	23.5860242037595
33	22.57432080163559	26.61515186255804	27.483376597852878	23.32715073795349
34	23.153376665607755	26.54367388943275	27.804470419393134	22.498479025566358
35	23.648067175735058	26.77597828690683	27.14215128599029	22.433803251367817
36	22.6542493096192	26.859216912137292	27.209653627258856	23.276880150984653
37	23.45585159415226	26.767638855810787	26.34723796512457	23.42927158491238
38	22.80255568119085	27.287587831922266	26.861606847358576	23.048249639528308
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1517.0
1	1209.5
2	902.0
3	902.0
4	1945.0
5	2988.0
6	2988.0
7	3487.0
8	3986.0
9	3950.0
10	3914.0
11	3914.0
12	5231.5
13	6549.0
14	8586.0
15	10623.0
16	10623.0
17	15508.5
18	20394.0
19	20394.0
20	25061.0
21	29728.0
22	33402.5
23	37077.0
24	37077.0
25	44313.5
26	51550.0
27	51550.0
28	66928.5
29	82307.0
30	99031.0
31	115755.0
32	115755.0
33	138338.5
34	160922.0
35	160922.0
36	170564.0
37	180206.0
38	194021.0
39	207836.0
40	207836.0
41	209748.0
42	211660.0
43	221887.0
44	232114.0
45	232114.0
46	232091.5
47	232069.0
48	232069.0
49	237868.5
50	243668.0
51	237197.5
52	230727.0
53	230727.0
54	217680.5
55	204634.0
56	204634.0
57	194407.5
58	184181.0
59	164055.5
60	143930.0
61	143930.0
62	132777.0
63	121624.0
64	100163.5
65	78703.0
66	78703.0
67	64914.5
68	51126.0
69	51126.0
70	41012.0
71	30898.0
72	24300.5
73	17703.0
74	17703.0
75	13727.5
76	9752.0
77	9752.0
78	9053.0
79	8354.0
80	6345.0
81	4336.0
82	4336.0
83	4330.0
84	4324.0
85	4324.0
86	2900.5
87	1477.0
88	1266.0
89	1055.0
90	1055.0
91	781.5
92	508.0
93	459.5
94	411.0
95	411.0
96	349.0
97	287.0
98	287.0
99	783.0
100	1279.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12445949846370306
2	0.05629335868012885
3	0.01228218734839175
4	0.003207015585413401
5	2.0470312247319582E-4
6	3.070546837097937E-4
7	6.823437415773195E-5
8	5.799921803407215E-4
9	4.0940624494639164E-4
10	6.141093674195874E-4
11	0.002524671843836082
12	0.003104664024176804
13	0.015693906056278347
14	0.009825749878713399
15	0.0245643746967835
16	0.012077484225918554
17	0.021562062233843295
18	0.006004624925880411
19	0.00788107021521804
20	0.006106976487117008
21	0.00685755460285206
22	0.007608132718587112
23	0.00999633581410773
24	0.014840976379306698
25	0.017672702906852574
26	0.019549148196190202
27	0.009450460820845873
28	0.00617521086127474
29	0.011497492045577833
30	0.003582304643280927
31	0.006209328048353608
32	0.007062257725325256
33	0.009075171762978349
34	0.013203351399521132
35	0.01678565604280206
36	0.015182148250095359
37	0.010235156123659791
38	0.006550499919142266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2931074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.456763726841814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44797599916774	34.85603243207252
2	14.811999251073635	14.058590975607189
3	5.223918062048641	7.437307355970711
4	2.329009196451465	4.421089566145555
5	1.2040399884883348	2.856992062568013
6	0.680440308905698	1.937489698197418
7	0.458158251918009	1.5219895537543078
8	0.309336252503614	1.1744077957768553
9	0.21941025186865007	0.9371250433959641
>10	1.1061335359685673	9.473365764829792
>50	0.0980764497674218	3.2572212476984386
>100	0.0930409355329362	9.410676680091804
>500	0.013124984248362328	4.2276396545443
>1k	0.005192301460853342	3.73701031681181
>5k	7.211529806740753E-5	0.2725224202133125
>10k+	7.211529806740753E-5	0.42053943232213786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12288	0.41923199482510504	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7963	0.2716751607090097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4378	0.1493650450312752	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3554	0.12125248287828967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.4117187078865974E-5	1.0235156123659791E-4
2	0.0	0.0	0.0	3.4117187078865974E-5	1.0235156123659791E-4
3	0.0	0.0	0.0	3.4117187078865974E-5	1.0235156123659791E-4
4	0.0	0.0	0.0	1.0235156123659791E-4	1.0235156123659791E-4
5	0.0	0.0	0.0	1.7058593539432987E-4	1.0235156123659791E-4
6	0.0	0.0	0.0	1.7058593539432987E-4	1.7058593539432987E-4
7	0.0	0.0	0.0	2.0470312247319582E-4	1.7058593539432987E-4
8	0.0	0.0	0.0	2.0470312247319582E-4	1.7058593539432987E-4
9	0.0	0.0	0.0	3.4117187078865974E-4	1.7058593539432987E-4
10	0.0	0.0	0.0	3.752890578675257E-4	1.7058593539432987E-4
11	0.0	0.0	0.0	4.0940624494639164E-4	1.7058593539432987E-4
12	0.0	0.0	0.0	4.435234320252576E-4	3.4117187078865974E-4
13	0.0	0.0	0.0	4.435234320252576E-4	4.0940624494639164E-4
14	0.0	0.0	0.0	4.776406191041236E-4	4.0940624494639164E-4
15	0.0	0.0	0.0	5.117578061829896E-4	5.458749932618556E-4
16	0.0	0.0	0.0	5.458749932618556E-4	5.458749932618556E-4
17	0.0	0.0	0.0	6.823437415773195E-4	5.799921803407215E-4
18	0.0	0.0	0.0	7.164609286561854E-4	5.799921803407215E-4
19	0.0	0.0	0.0	8.529296769716493E-4	5.799921803407215E-4
20	0.0	0.0	0.0	8.870468640505152E-4	6.482265544984535E-4
21	0.0	0.0	0.0	0.001228218734839175	6.823437415773195E-4
22	0.0	0.0	0.0	0.0019105624764164943	7.505781157350514E-4
23	0.0	0.0	0.0	0.0032070155854134015	7.846953028139174E-4
24	0.0	0.0	0.0	0.0050493436876721635	7.846953028139174E-4
25	0.0	0.0	0.0	0.006106976487117009	7.846953028139174E-4
26	0.0	0.0	0.0	0.007301078034877318	7.846953028139174E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	315	0.0	18.28391	7
ATACGAC	55	0.0013668574	17.451633	3
GATATAC	740	0.0	16.6709	1
AGAACCG	465	0.0	16.16769	5
CGCGGTC	390	0.0	15.179804	10
ATCGTTT	550	0.0	14.5447645	29
GTCCTAA	1175	0.0	14.453335	1
GTCCTAC	1975	0.0	14.277285	1
TTAGGAC	1425	0.0	14.257269	3
GAACCGC	455	0.0	14.062147	6
ACGTTTT	935	0.0	14.031421	29
TATACAC	1145	0.0	13.971467	3
CTAGCGG	460	0.0	13.912383	29
TAGGACG	2090	0.0	13.852729	4
TAGGACA	710	0.0	13.742778	4
GCATCGT	585	0.0	13.673398	27
TCACGTT	995	0.0	13.666528	27
CACGTTT	985	0.0	13.643091	28
CATCGTT	575	0.0	13.633205	28
CGTTATT	365	0.0	13.596516	2
>>END_MODULE