FastQCFastQC Report
Thu 2 Feb 2017
SRR4062922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931074
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT141130.4814958612440355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT92080.3141510586221979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT55790.19033978671299326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.14202984980931904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA22300.015.7839722
TTCGTAC2350.015.65879824
TAGCACG650.004162636614.7697584
CTAGCGG4550.014.76849729
GTAGGAC22550.014.7580873
TCTAGCG4650.014.45089728
GTCCTAC19350.014.2237011
TACCGTC5100.014.1198387
CTGTAGG24050.014.1054161
ACCGTCG5000.014.0821848
GTATTAG7750.014.040171
CGAGCCG7350.013.93128115
TAGGACG21400.013.832274
GATATAC8400.013.7157131
ATCGTTT4850.013.52510229
TAGCGGC5000.013.43933430
CGTCGTA5550.013.26286710
GCTCGTT3500.013.25693812
GTATCAA90550.013.2183811
GGACGTG21750.013.1698596
Produced by FastQC (version 0.11.5)