##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2931074
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16956071392261	32.0	32.0	32.0	32.0	32.0
2	31.30777626221651	32.0	32.0	32.0	32.0	32.0
3	31.39295425499322	32.0	32.0	32.0	32.0	32.0
4	31.487559508903562	32.0	32.0	32.0	32.0	32.0
5	31.418701131394158	32.0	32.0	32.0	32.0	32.0
6	34.92568526076107	36.0	36.0	36.0	36.0	36.0
7	34.956808323501896	36.0	36.0	36.0	36.0	36.0
8	34.90282503955888	36.0	36.0	36.0	36.0	36.0
9	35.00503023806291	36.0	36.0	36.0	36.0	36.0
10	34.86581573853134	36.0	36.0	36.0	32.0	36.0
11	35.034570945667014	36.0	36.0	36.0	36.0	36.0
12	34.93929972426489	36.0	36.0	36.0	36.0	36.0
13	34.98607438775002	36.0	36.0	36.0	36.0	36.0
14	34.94169816251654	36.0	36.0	36.0	32.0	36.0
15	34.91331334521066	36.0	36.0	36.0	32.0	36.0
16	34.92405172984374	36.0	36.0	36.0	36.0	36.0
17	34.903207152054165	36.0	36.0	36.0	32.0	36.0
18	34.89661298213556	36.0	36.0	36.0	32.0	36.0
19	34.88308176456821	36.0	36.0	36.0	32.0	36.0
20	34.87130246455736	36.0	36.0	36.0	32.0	36.0
21	34.85895647806913	36.0	36.0	36.0	32.0	36.0
22	34.84102004930616	36.0	36.0	36.0	32.0	36.0
23	34.794440877302996	36.0	36.0	36.0	32.0	36.0
24	34.78493071140476	36.0	36.0	36.0	32.0	36.0
25	34.772653641634435	36.0	36.0	36.0	32.0	36.0
26	34.70790365579307	36.0	36.0	36.0	32.0	36.0
27	34.68337271593962	36.0	36.0	36.0	32.0	36.0
28	34.65116165610285	36.0	36.0	36.0	32.0	36.0
29	34.62241656130142	36.0	36.0	36.0	32.0	36.0
30	34.59725104176831	36.0	36.0	36.0	32.0	36.0
31	34.587347504703054	36.0	36.0	36.0	32.0	36.0
32	34.557337685776616	36.0	36.0	36.0	32.0	36.0
33	34.53295652037444	36.0	36.0	36.0	32.0	36.0
34	34.50729050170688	36.0	36.0	36.0	32.0	36.0
35	34.47746184504383	36.0	36.0	36.0	32.0	36.0
36	34.441031512681015	36.0	36.0	36.0	32.0	36.0
37	34.421024170662356	36.0	36.0	36.0	32.0	36.0
38	34.0171162515856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	19.0
21	69.0
22	221.0
23	644.0
24	1779.0
25	4523.0
26	9647.0
27	18754.0
28	32912.0
29	52375.0
30	79813.0
31	115495.0
32	166192.0
33	264241.0
34	645745.0
35	1538640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.627192274608696	19.79658817054575	12.717913827712064	25.858305727133484
2	14.532318187804197	21.592733585027197	39.06080160378073	24.814146623387877
3	17.44360599561799	26.033119600528682	29.85189046745323	26.671383936400105
4	11.50355826052424	17.41410210002023	37.934410915888314	33.14792872356722
5	12.97587127708609	38.265603255601796	34.16157438697323	14.596951080338894
6	31.629609664729948	37.87596338538486	17.565974200547924	12.928452749337271
7	28.154371930068773	32.125302493883595	21.789419563153288	17.93090601289435
8	26.44215776921791	34.95272928259735	19.984169171508604	18.620943776676143
9	27.007396893850476	14.834388732701914	19.645815705434398	38.51239866801321
10	15.25821227838056	27.711547922613082	32.917007558414454	24.113232240591902
11	35.37353041325506	22.110111275412617	24.173691181558322	18.342667129774004
12	24.437934100336328	24.935705897725807	29.919019113208794	20.707340888729068
13	28.646739927739702	20.963573029644465	26.259727676241102	24.129959366374738
14	22.45641017592869	21.49720546120637	26.70365879537671	29.34272556748823
15	23.812977768558554	29.13887537469201	23.61216400541235	23.435982851337087
16	23.777853290536704	27.09435319227914	25.847369153674833	23.280424363509322
17	22.192036093254554	27.298253131787188	26.921804089558982	23.587906685399275
18	22.777725843837445	25.999002413449812	28.973850540791535	22.24942120192121
19	24.460487862128353	25.797642775310347	27.154005664817742	22.587863697743558
20	24.453903245022133	25.99801301502453	26.996316026139223	22.551767713814115
21	25.199355457442007	25.23435972721234	26.198512420886427	23.367772394459227
22	24.168922381352363	26.238812121427163	26.704682310989075	22.887583186231396
23	22.866598204751163	26.040592684582762	27.345645105712247	23.74716400495382
24	23.559077662317634	26.392510049217456	27.20190619547647	22.84650609298844
25	23.463214274382484	25.954762579795972	27.556464893866366	23.025558251955175
26	22.608955832898054	26.747508506270574	28.10496360888999	22.53857205194139
27	23.296118280274246	26.73803304729464	26.88112062753832	23.084728044892792
28	22.54057727645225	26.575412289147256	27.470817864032092	23.413192570368405
29	23.050253934223427	26.10490216214261	27.124494297994524	23.720349605639434
30	22.869944600511623	26.281424488088668	27.643075541593287	23.205555369806426
31	23.547648404646214	25.841039837274664	26.90460902727123	23.706702730807887
32	22.734874656866392	26.729963146614516	26.952100151685016	23.583062044834076
33	22.626416119142675	26.45166925161221	27.55658847234836	23.365326156896753
34	23.142029167465576	26.320011026674866	27.903833202437063	22.6341266034225
35	23.69759344185783	26.550063219147656	27.187781680025818	22.564561658968692
36	22.696799876086377	26.57261467980679	27.40517639609235	23.325409048014482
37	23.59595834154989	26.512124907115957	26.459516204640348	23.432400546693806
38	22.96318409318099	26.883697762250648	27.01907494532713	23.134043199241233
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	915.0
1	720.0
2	525.0
3	525.0
4	1610.5
5	2696.0
6	2696.0
7	3260.5
8	3825.0
9	4002.5
10	4180.0
11	4180.0
12	5343.0
13	6506.0
14	8653.5
15	10801.0
16	10801.0
17	16192.5
18	21584.0
19	21584.0
20	26248.0
21	30912.0
22	34245.0
23	37578.0
24	37578.0
25	44320.5
26	51063.0
27	51063.0
28	66465.0
29	81867.0
30	98347.0
31	114827.0
32	114827.0
33	137833.0
34	160839.0
35	160839.0
36	171046.0
37	181253.0
38	194903.5
39	208554.0
40	208554.0
41	210348.5
42	212143.0
43	222694.0
44	233245.0
45	233245.0
46	233236.5
47	233228.0
48	233228.0
49	238752.5
50	244277.0
51	237235.0
52	230193.0
53	230193.0
54	217628.5
55	205064.0
56	205064.0
57	194509.0
58	183954.0
59	164121.5
60	144289.0
61	144289.0
62	132852.0
63	121415.0
64	99899.0
65	78383.0
66	78383.0
67	64676.0
68	50969.0
69	50969.0
70	40895.0
71	30821.0
72	24244.5
73	17668.0
74	17668.0
75	13553.0
76	9438.0
77	9438.0
78	8821.5
79	8205.0
80	6233.5
81	4262.0
82	4262.0
83	3959.5
84	3657.0
85	3657.0
86	2344.0
87	1031.0
88	838.0
89	645.0
90	645.0
91	394.5
92	144.0
93	106.5
94	69.0
95	69.0
96	47.0
97	25.0
98	25.0
99	24.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00924575769837268
2	0.0
3	0.0
4	2.388203095520618E-4
5	1.0235156123659791E-4
6	1.364687483154639E-4
7	7.846953028139175E-4
8	0.0028658437146247416
9	0.0038893593269907207
10	0.0012282187348391749
11	0.005049343687672164
12	4.0940624494639164E-4
13	1.364687483154639E-4
14	0.0
15	0.0
16	6.823437415773195E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0235156123659791E-4
22	0.0
23	1.364687483154639E-4
24	0.0
25	1.7058593539432984E-4
26	3.4117187078865974E-5
27	6.823437415773195E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.364687483154639E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2931074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.20457892774172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20890288948286	32.18968471025523
2	15.834359064796482	14.315710682295906
3	5.780019547172851	7.838500494721953
4	2.566333205394433	4.640400477525483
5	1.3424229390181739	3.0341831850629024
6	0.7843403161265106	2.127346423593045
7	0.4932804081866226	1.5608973201766585
8	0.3417732138399915	1.2359771376334276
9	0.23215778758064054	0.9445135529141064
>10	1.1934848828102684	9.764472164908321
>50	0.10192388630591345	3.2428357986948804
>100	0.10068274645064212	9.75418732476398
>500	0.014253706018468041	4.45048875789416
>1k	0.005837954060726964	3.9103116873762556
>5k	1.5163517040849255E-4	0.5067951488805859
>10k+	7.581758520424628E-5	0.4836951333030168
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	14113	0.4814958612440355	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9208	0.3141510586221979	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5579	0.19033978671299326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4163	0.14202984980931904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0235156123659791E-4
2	0.0	0.0	0.0	0.0	1.0235156123659791E-4
3	0.0	0.0	0.0	0.0	1.0235156123659791E-4
4	0.0	0.0	0.0	0.0	1.0235156123659791E-4
5	3.4117187078865974E-5	0.0	0.0	0.0	1.0235156123659791E-4
6	3.4117187078865974E-5	0.0	0.0	0.0	1.364687483154639E-4
7	3.4117187078865974E-5	0.0	0.0	0.0	1.364687483154639E-4
8	3.4117187078865974E-5	0.0	0.0	0.0	1.364687483154639E-4
9	3.4117187078865974E-5	0.0	0.0	3.4117187078865974E-5	1.7058593539432987E-4
10	3.4117187078865974E-5	0.0	0.0	3.4117187078865974E-5	1.7058593539432987E-4
11	3.4117187078865974E-5	0.0	0.0	6.823437415773195E-5	1.7058593539432987E-4
12	3.4117187078865974E-5	0.0	0.0	6.823437415773195E-5	4.0940624494639164E-4
13	3.4117187078865974E-5	0.0	0.0	6.823437415773195E-5	4.776406191041236E-4
14	3.4117187078865974E-5	0.0	0.0	1.0235156123659791E-4	4.776406191041236E-4
15	3.4117187078865974E-5	0.0	0.0	1.364687483154639E-4	5.458749932618556E-4
16	3.4117187078865974E-5	0.0	0.0	1.7058593539432987E-4	5.458749932618556E-4
17	3.4117187078865974E-5	0.0	0.0	3.0705468370979377E-4	5.458749932618556E-4
18	3.4117187078865974E-5	0.0	0.0	3.4117187078865974E-4	5.458749932618556E-4
19	3.4117187078865974E-5	0.0	0.0	3.4117187078865974E-4	5.458749932618556E-4
20	3.4117187078865974E-5	0.0	0.0	4.0940624494639164E-4	6.141093674195875E-4
21	3.4117187078865974E-5	0.0	0.0	6.823437415773195E-4	6.141093674195875E-4
22	3.4117187078865974E-5	0.0	0.0	0.0012623359219180409	6.482265544984535E-4
23	3.4117187078865974E-5	0.0	0.0	0.00245643746967835	6.823437415773195E-4
24	3.4117187078865974E-5	0.0	0.0	0.004298765571937113	7.164609286561854E-4
25	3.4117187078865974E-5	0.0	0.0	0.00525404681014536	7.164609286561854E-4
26	3.4117187078865974E-5	0.0	0.0	0.0063799139837479365	7.164609286561854E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	2230	0.0	15.783972	2
TTCGTAC	235	0.0	15.658798	24
TAGCACG	65	0.0041626366	14.769758	4
CTAGCGG	455	0.0	14.768497	29
GTAGGAC	2255	0.0	14.758087	3
TCTAGCG	465	0.0	14.450897	28
GTCCTAC	1935	0.0	14.223701	1
TACCGTC	510	0.0	14.119838	7
CTGTAGG	2405	0.0	14.105416	1
ACCGTCG	500	0.0	14.082184	8
GTATTAG	775	0.0	14.04017	1
CGAGCCG	735	0.0	13.931281	15
TAGGACG	2140	0.0	13.83227	4
GATATAC	840	0.0	13.715713	1
ATCGTTT	485	0.0	13.525102	29
TAGCGGC	500	0.0	13.439334	30
CGTCGTA	555	0.0	13.262867	10
GCTCGTT	350	0.0	13.256938	12
GTATCAA	9055	0.0	13.218381	1
GGACGTG	2175	0.0	13.169859	6
>>END_MODULE